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Literature summary extracted from

  • Kushwaha, N.; Srivastava, S.
    Gamma-glutamyl transpeptidase from two plant growth promoting rhizosphere fluorescent pseudomonads (2014), Antonie van Leeuwenhoek, 105, 45-56.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.2.2 Na+ 0.2 M, 80% residual activity Pseudomonas fluorescens
3.4.19.13 Na+ 0.2 M, 80% residual activity Pseudomonas fluorescens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.2.2 periplasm
-
 Pseudomonas fluorescens
-
-
3.4.19.13 periplasm
-
 Pseudomonas fluorescens
-
-
3.4.19.13 periplasm
-
 Pseudomonas protegens
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.2.2 Ca2+ 50 mM, 320% of initial activity Pseudomonas fluorescens
2.3.2.2 Co2+ 1 mM, 217% of initial activity Pseudomonas fluorescens
2.3.2.2 Mg2+ 50 mM, 313% of initial activity Pseudomonas fluorescens
2.3.2.2 Mn2+ 50 mM, 301% of initial activity Pseudomonas fluorescens
3.4.19.13 Ca2+ 50 mM, 320% of initial activity Pseudomonas fluorescens
3.4.19.13 Co2+ 1 mM, 217% of initial activity Pseudomonas fluorescens
3.4.19.13 Mg2+ 50 mM, 313% of initial activity Pseudomonas fluorescens
3.4.19.13 Mn2+ 50 mM, 301% of initial activity Pseudomonas fluorescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.2.2 23000
-
 and 40000, SDS-PAGE, small and large subunit Pseudomonas fluorescens
2.3.2.2 40000
-
 and 23000, SDS-PAGE, large and small subunit Pseudomonas fluorescens
2.3.2.2 57330
-
 unprocessed protein, calculated Pseudomonas fluorescens
3.4.19.13 23000
-
 and 40000, SDS-PAGE, small and large subunit Pseudomonas fluorescens
3.4.19.13 40000
-
 and 23000, SDS-PAGE, large and small subunit Pseudomonas fluorescens
3.4.19.13 57330
-
 unprocessed protein, calculated Pseudomonas fluorescens

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.2 Pseudomonas fluorescens Q4KIM5 enzyme displays both glutathione hydrolase, EC 3.4.19.13, and gamma-glutamyl transpeptidase activity
-
2.3.2.2 Pseudomonas fluorescens Pf-5 Q4KIM5 enzyme displays both glutathione hydrolase, EC 3.4.19.13, and gamma-glutamyl transpeptidase activity
-
3.4.19.13 Pseudomonas fluorescens Q4KIM5 enzyme displays both glutathione hydrolase and gamma-glutamyl transpeptidase activity, EC 2.3.2.2
-
3.4.19.13 Pseudomonas fluorescens Pf-5 Q4KIM5 enzyme displays both glutathione hydrolase and gamma-glutamyl transpeptidase activity, EC 2.3.2.2
-
3.4.19.13 Pseudomonas protegens L7Z147 enzyme displays both glutathione hydrolase and gamma-glutamyl transpeptidase activity, EC 2.3.2.2
-
3.4.19.13 Pseudomonas protegens PfT-1 L7Z147 enzyme displays both glutathione hydrolase and gamma-glutamyl transpeptidase activity, EC 2.3.2.2
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.3.2.2 proteolytic modification protein is processed to subunits of 23000 and 40000 Da Pseudomonas fluorescens
3.4.19.13 proteolytic modification protein is processed to subunits of 23000 and 40000 Da Pseudomonas fluorescens
3.4.19.13 proteolytic modification protein is processed to subunits of 23000 and 40000 Da Pseudomonas protegens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.19.13 31.06
-
 pH 8.0, 50°C Pseudomonas fluorescens
3.4.19.13 31.06
-
 pH 8.0, 50°C Pseudomonas protegens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.19.13 L-glutamic acid 4-nitroanilide + H2O
-
 Pseudomonas fluorescens 4-nitroaniline + L-glutamate
-
 ?
3.4.19.13 L-glutamic acid 4-nitroanilide + H2O
-
 Pseudomonas fluorescens Pf-5 4-nitroaniline + L-glutamate
-
 ?
3.4.19.13 L-glutamic acid-(4-nitroanilide) + H2O
-
 Pseudomonas protegens 4-nitroaniline + L-glutamate
-
 ?
3.4.19.13 L-glutamic acid-(4-nitroanilide) + H2O
-
 Pseudomonas protegens PfT-1 4-nitroaniline + L-glutamate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.19.13 dimer 1 * 23000 + 1 * 40000, SDS-PAGE Pseudomonas fluorescens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.2.2 50
-
-
 Pseudomonas fluorescens
3.4.19.13 50
-
-
 Pseudomonas fluorescens
3.4.19.13 50
-
-
 Pseudomonas protegens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.3.2.2 30 60
-
 Pseudomonas fluorescens
3.4.19.13 30 60
-
 Pseudomonas fluorescens
3.4.19.13 30 60
-
 Pseudomonas protegens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.2.2 50
-
 24 h, 50% residual activity Pseudomonas fluorescens
3.4.19.13 50
-
 24 h, 50% residual activity Pseudomonas fluorescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.2.2 8
-
-
 Pseudomonas fluorescens
3.4.19.13 8
-
-
 Pseudomonas fluorescens
3.4.19.13 8
-
-
 Pseudomonas protegens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.2.2 4 12
-
 Pseudomonas fluorescens
3.4.19.13 4 12
-
 Pseudomonas fluorescens
3.4.19.13 4 12
-
 Pseudomonas protegens