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Literature summary extracted from

  • Wiater, A.; Pleszczynska, M.; Rogalski, J.; Szajnecka, L.; Szczodrak, J.
    Purification and properties of an alpha-(1->3)-glucanase (EC 3.2.1.84) from Trichoderma harzianum and its use for reduction of artificial dental plaque accumulation (2013), Acta Biochim. Pol., 60, 123-128.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.84 Ca2+ moderate inhibition at 1 mM Trichoderma harzianum
3.2.1.84 Co2+ moderate inhibition at 1 mM Trichoderma harzianum
3.2.1.84 Cu2+ moderate inhibition at 1 mM Trichoderma harzianum
3.2.1.84 Fe3+ moderate inhibition at 1 mM Trichoderma harzianum
3.2.1.84 Hg2+ complete inhibition at 1 mM Trichoderma harzianum
3.2.1.84 Mn2+ moderate inhibition at 1 mM Trichoderma harzianum
3.2.1.84 Sn2+ moderate inhibition at 1 mM Trichoderma harzianum
3.2.1.84 Zn2+ moderate inhibition at 1 mM Trichoderma harzianum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.84 additional information
-
 additional information Km is 0.73 mg/ml with lyophilized Laetiporus sulphureus cell walls, pH 5.5, 45°C Trichoderma harzianum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.84 extracellular
-
 Trichoderma harzianum
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.84 Mg2+ activates slightly Trichoderma harzianum
3.2.1.84 additional information no or poor effects by Ba2+, K+, and Fe2+ Trichoderma harzianum
3.2.1.84 Na+ activates slightly Trichoderma harzianum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.84 67000
-
 x * 67000, SDS-PAGE Trichoderma harzianum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.84 additional information Trichoderma harzianum the enzyme preferentially catalyzes the hydrolysis of various streptococcal mutans and fungal alpha-(1->3)-glucans ?
-
 ?
3.2.1.84 additional information Trichoderma harzianum CCM F-340 the enzyme preferentially catalyzes the hydrolysis of various streptococcal mutans and fungal alpha-(1->3)-glucans ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.84 Trichoderma harzianum
-
-
-
3.2.1.84 Trichoderma harzianum CCM F-340
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.84 native extracellular enzyme 18.4fold by ultrafiltration, anion exchange and hydrophobic interaction chromatography, and chromatofocusing to homogeneity Trichoderma harzianum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.84 mycelium
-
 Trichoderma harzianum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.84 115.6
-
 purified enzyme with lyophilized Laetiporus sulphureus cell walls, pH 5.5, 45°C Trichoderma harzianum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.84 additional information the enzyme preferentially catalyzes the hydrolysis of various streptococcal mutans and fungal alpha-(1->3)-glucans Trichoderma harzianum ?
-
 ?
3.2.1.84 additional information alpha-(1,3)-endo-glucanase activity with lyophilized Laetiporus sulphureus cell walls prepared from fruiting bodies, also glucans are iso­lated from fruiting bodies of Pleurotus precoce, Pleurotus djamor, Pleurotus citrinopileatus, Pleurotus eryngii, Piptoporus betulinus, Laetiporus sul­phureus and Hericium erinaceus, and from mycelia of Aspergillus fumigatus CCM F-614, Aspergillus wentii CIM 449, and Aspergillus nidulans DIM AN1, and with dextranase-pretreated mutan synthesized from su­crose using cell-free glucosyltransferases of Streptococci, substrate specificity, overview Trichoderma harzianum ?
-
 ?
3.2.1.84 additional information the enzyme preferentially catalyzes the hydrolysis of various streptococcal mutans and fungal alpha-(1->3)-glucans Trichoderma harzianum CCM F-340 ?
-
 ?
3.2.1.84 additional information alpha-(1,3)-endo-glucanase activity with lyophilized Laetiporus sulphureus cell walls prepared from fruiting bodies, also glucans are iso­lated from fruiting bodies of Pleurotus precoce, Pleurotus djamor, Pleurotus citrinopileatus, Pleurotus eryngii, Piptoporus betulinus, Laetiporus sul­phureus and Hericium erinaceus, and from mycelia of Aspergillus fumigatus CCM F-614, Aspergillus wentii CIM 449, and Aspergillus nidulans DIM AN1, and with dextranase-pretreated mutan synthesized from su­crose using cell-free glucosyltransferases of Streptococci, substrate specificity, overview Trichoderma harzianum CCM F-340 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.84 ? x * 67000, SDS-PAGE Trichoderma harzianum

Synonyms

EC Number Synonyms Comment Organism
3.2.1.84 alpha-(1->3)-glucanase
-
 Trichoderma harzianum
3.2.1.84 mutanase
-
 Trichoderma harzianum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.84 45
-
-
 Trichoderma harzianum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.84 45
-
 purified enzyme, pH 5.5, 1 h, stable Trichoderma harzianum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.84 5.5
-
-
 Trichoderma harzianum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.84 4.5 6 purified enzyme, 45°C, 1 h, stable at Trichoderma harzianum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.84 Trichoderma harzianum isoelectric focusing
-
 7.1