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Literature summary extracted from

  • Rosnes, I.; Rowe, A.D.; Vik, E.S.; Forstrom, R.J.; Alseth, I.; Bjoras, M.; Dalhus, B.
    Structural basis of DNA loop recognition by endonuclease V (2013), Structure, 21, 257-265.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.21.7 in complex with DNA containing one nucleotide A:TT loop, vapor diffusion method, using 15% (w/v) polyethylene glycol 400 and 100 mM MES, pH 6.3 Thermotoga maritima

Protein Variants

EC Number Protein Variants Comment Organism
3.1.21.7 D43A active site mutant Thermotoga maritima

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.21.7 DNA + H2O Thermotoga maritima the enzyme binds A:TT loops with higher affinity than undamaged DNA ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.21.7 Thermotoga maritima Q9X2H9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.21.7 HiTrap SP column chromatography, and Superdex 75 gel filtration Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.21.7 DNA + H2O the enzyme binds A:TT loops with higher affinity than undamaged DNA Thermotoga maritima ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.21.7 endonuclease V
-
 Thermotoga maritima
3.1.21.7 EndoV
-
 Thermotoga maritima

General Information

EC Number General Information Comment Organism
3.1.21.7 physiological function the DNA repair enzyme recognizes and cleaves DNA at deaminated adenine lesions (hypoxanthine) In addition, the enzyme cleaves DNA containing various helical distortions such as loops, hairpins, and flaps Thermotoga maritima