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Literature summary extracted from

  • Romanova, J.; Filimonova, M.
    The effects of addition of mononucleotides on Sma nuc endonuclease activity (2012), ScientificWorldJournal, 2012, 454176.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.30.2 AMP competitive inhibitor of partial type, AMP forming a complex with Sma nuc does not completely prevent the RNA binding and reduces the enzyme affinity to RNA substrate in competition with RNA for the binding site Serratia marcescens
3.1.30.2 CMP uncompetitive inhibitor Serratia marcescens
3.1.30.2 dAMP competitive inhibitor of partial type, dAMP forming a complex with Sma nuc does not completely prevent the RNA binding and reduces the enzyme affinity to RNA substrate in competition with RNA for the binding site Serratia marcescens
3.1.30.2 GMP uncompetitive inhibitor Serratia marcescens
3.1.30.2 UMP uncompetitive inhibitor Serratia marcescens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.30.2 Mg2+ required Serratia marcescens

Organism

EC Number Organism UniProt Comment Textmining
3.1.30.2 Serratia marcescens P13717 isoform Sm2 of Sma nuc endonuclease
-

Synonyms

EC Number Synonyms Comment Organism
3.1.30.2 Sma nuc endonuclease
-
 Serratia marcescens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.30.2 37
-
 assay at Serratia marcescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.30.2 8.5
-
 assay at Serratia marcescens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.30.2 additional information
-
 additional information inhibition kinetics of mononucleotides, hyperbolic inhibition of the enzyme, overview Serratia marcescens
3.1.30.2 0.06
-
 dAMP pH 8.5, 37°C Serratia marcescens
3.1.30.2 0.0765
-
 AMP pH 8.5, 37°C Serratia marcescens
3.1.30.2 0.0862
-
 CMP pH 8.5, 37°C Serratia marcescens
3.1.30.2 0.229
-
 GMP pH 8.5, 37°C Serratia marcescens
3.1.30.2 0.3107
-
 UMP pH 8.5, 37°C Serratia marcescens

General Information

EC Number General Information Comment Organism
3.1.30.2 additional information specific site(s) for the nucleotide(s) binding in Sma nuc endonuclease Serratia marcescens
3.1.30.2 physiological function any mononucleotide produced by Sma nuc during hydrolysis of DNA or RNA may regulate the enzyme activity affecting the RNase activity without pronounced influence on the activity towards DNA. The type of carbohydrate residue in mononucleotides does not affect the regulation. In contrast, the effects depend on the type of bases in nucleotides Serratia marcescens