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Literature summary extracted from

  • Suzuki, K.; Hori, A.; Kawamoto, K.; Thangudu, R.R.; Ishida, T.; Igarashi, K.; Samejima, M.; Yamada, C.; Arakawa, T.; Wakagi, T.; Koseki, T.; Fushinobu, S.
    Crystal structure of a feruloyl esterase belonging to the tannase family: A disulfide bond near a catalytic triad (2014), Proteins, 82, 2857-2867.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.73 recombinant expression in Pichia pastoris strain KM71H, the enzyme is secreted Aspergillus oryzae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.1.73 purified recombinant deglycosylated enzyme, untreated or as iodine derivative, mixing of 0.001 ml of 30 mg/ml protein in 5 mM HEPES-NaOH, pH 7.0, with 0.001 ml of reservoir solution containing 20% PEG 1000 and 0.1M Tris-HCl, pH 7.0, hanging drop vapor diffusion method, 20°C, X-ray diffraction structure determination and analysis at 1.5-2.4 A resolution, modeling Aspergillus oryzae

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.73 C202A site-directed mutagenesis, almost inactive mutant Aspergillus oryzae
3.1.1.73 C202A/C458A site-directed mutagenesis, almost inactive mutant Aspergillus oryzae
3.1.1.73 C458A site-directed mutagenesis, almost inactive mutant Aspergillus oryzae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.73 55000
-
 2 * 55000, recombinant deglycosylated enzyme, SDS-PAGE, 2 * 67000, recombinant glycosylated enzyme, SDS-PAGE Aspergillus oryzae
3.1.1.73 67000
-
 2 * 55000, recombinant deglycosylated enzyme, SDS-PAGE, 2 * 67000, recombinant glycosylated enzyme, SDS-PAGE Aspergillus oryzae
3.1.1.73 101000
-
 recombinant deglycosylated enzyme, gel filtration Aspergillus oryzae

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.73 Aspergillus oryzae
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.73 glycoprotein the recombinant extracellular enzyme is deglycosylated by endoglycosidase H Aspergillus oryzae

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.73 recombinant extracellular enzyme from Pichia pastoris strain KM71H culture supernatant by ammonium sulfate fractionation, anion exchange chromatography, and deglycosylation with endoglycosidase H, followed by hydrophobic interaction chromatography Aspergillus oryzae

Subunits

EC Number Subunits Comment Organism
3.1.1.73 dimer 2 * 55000, recombinant deglycosylated enzyme, SDS-PAGE, 2 * 67000, recombinant glycosylated enzyme, SDS-PAGE Aspergillus oryzae

Synonyms

EC Number Synonyms Comment Organism
3.1.1.73 AoFaeB
-
 Aspergillus oryzae
3.1.1.73 FAE B
-
 Aspergillus oryzae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.73 37
-
 assay at Aspergillus oryzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.73 7
-
 assay at Aspergillus oryzae

General Information

EC Number General Information Comment Organism
3.1.1.73 evolution the enzyme belongs to the fungal tannase family Aspergillus oryzae
3.1.1.73 additional information the enzyme structure consists of a catalytic alpha/beta-hydrolase fold domain and a large lid domain with a unique fold, binding models of substrates and docking analysis, overview. The catalytic triad of the enzyme comprises residues Ser203, Asp417, and His457, and the serine and histidine residues are directly connected by a disulfide bond of the neighboring cysteine residues, Cys202 and Cys458. The CS-D-HC structural motif plays an essential role in the function of the active site Aspergillus oryzae