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Literature summary extracted from

  • Chaudet, M.M.; Naumann, T.A.; Price, N.P.; Rose, D.R.
    Crystallographic structure of ChitA, a glycoside hydrolase family 19, plant class IV chitinase from Zea mays (2014), Protein Sci., 23, 586-593.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.14 gene chiA, cloning of wild-type and mutant enzymes in Escherichia coli strain DH5alpha, heterologous expression in Pichia pastoris strain X-33 Zea mays

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.14 purified recombinant wild-type and mutant E64Q enzymes, sitting drop vapour diffusion method, mixing of 26 mg/ml protein in sodium acetate buffer, pH 5.2, with reservoir solution, X-ray diffraction structure determination and analysis Zea mays

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.14 E62Q site-directed mutagenesis of wild-type enzyme and mutant ChitA DELTAN, the mutation abolishes the chitinase activity of both full-length and truncated proteins without disrupting substrate binding Zea mays
3.2.1.14 additional information construction of a truncated enzyme mutant, ChitA DELTAN, which lacks the hevein-like domain. The mutant enzyme activity is comparable to the wild-type full-length enzyme Zea mays

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.14 28000
-
 x * 28000 Zea mays

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.14 chitin + H2O Zea mays
-
 ?
-
 ?
3.2.1.14 chitin + H2O Zea mays LH82
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Zea mays P29022 gene CHIA
-
3.2.1.14 Zea mays LH82 P29022 gene CHIA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.14 recombinant wild-type and mutant enzymes from Pichia pastoris strain X-33 by ammonium sulfate fractionation, cation exchange chromatography, and dialysis Zea mays

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.14 seed
-
 Zea mays
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 chitin + H2O
-
 Zea mays ?
-
 ?
3.2.1.14 chitin + H2O
-
 Zea mays LH82 ?
-
 ?
3.2.1.14 additional information enzyme activity on the modified chitopentaose substrate GlcN-GlcNAc4, which contains a non-acetylated glucosamine residue at the nonreducing end, and on the modified chitotetraose substrate TMG-chitotriomycin-pMP, which contains a N-trimethylamino glucosamine (TMG) residue at the non-reducing end, and a p-methyoxyphenyl protecting group at the anomeric position, cleavage product is TMG-GlcNAc2, MALDI-TOF mass spectrometry, overview Zea mays ?
-
 ?
3.2.1.14 additional information enzyme activity on the modified chitopentaose substrate GlcN-GlcNAc4, which contains a non-acetylated glucosamine residue at the nonreducing end, and on the modified chitotetraose substrate TMG-chitotriomycin-pMP, which contains a N-trimethylamino glucosamine (TMG) residue at the non-reducing end, and a p-methyoxyphenyl protecting group at the anomeric position, cleavage product is TMG-GlcNAc2, MALDI-TOF mass spectrometry, overview Zea mays LH82 ?
-
 ?
3.2.1.14 p-methoxyphenyl TMG-chitotriomycin + H2O a synthetic compound and an enzyme inhibitor specific for insect and fungal b-N-acetylglucosaminidases Zea mays ?
-
 ?
3.2.1.14 p-methoxyphenyl TMG-chitotriomycin + H2O a synthetic compound and an enzyme inhibitor specific for insect and fungal b-N-acetylglucosaminidases Zea mays LH82 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.14 ? x * 28000 Zea mays

Synonyms

EC Number Synonyms Comment Organism
3.2.1.14 ChitA
-
 Zea mays
3.2.1.14 class IV chitinase
-
 Zea mays

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.14 37
-
 assay at Zea mays

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 5
-
 assay at Zea mays

General Information

EC Number General Information Comment Organism
3.2.1.14 evolution the enzyme is a glycoside hydrolase family 19 plant class IV chitinase. Family GH19 chitinases operate by an inverting glycoside mechanism. Comparison of the crystal structure of this plant class IV chitinase with structures from larger class I and II enzymes suggest that class IV chitinases have evolved to accommodate shorter substrates Zea mays
3.2.1.14 additional information key roles for Glu62, Arg177, and Glu165 in hydrolysis, and for Ser103 and Tyr106 in substrate binding. Glu62 is directly involved in catalysis, while the hevein-like domain is not needed for enzyme activity. Proton donor and general base are Glu62 and Glu71, respectively. The catalytic triad highly conserved in GH19 chitinases, is composed of Glu62, Arg177, and Glu165 Zea mays
3.2.1.14 physiological function the enzyme has in vitro antifungal activity in healthy maize seeds Zea mays