Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Light, S.H.; Anderson, W.F.; Lavie, A.
    Reassessing the type I dehydroquinate dehydratase catalytic triad: kinetic and structural studies of Glu86 mutants (2013), Protein Sci., 22, 418-424.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.1.10 mutant enzymes E86A and E86Q, sitting drop vapor diffusion method, using 0.2 M sodium chloride and 20% (w/v) PEG 3350 (unliganded structure), 0.19 M sodium chloride and 17.9% (w/v) PEG 3350 (cocrystallized E86A-3-dehydroshikimate complex), 0.1 M MIB buffer (pH 9.0) and 25% (w/v) PEG 1500 (soaked E86A-3-dehydroshikimate complex) Salmonella enterica

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.10 E86A the mutant retains catalytic proficiency but is optimally active at more acidic conditions than the wild type enzyme (pH 5.7).The mutation reduces kcat 17fold Salmonella enterica
4.2.1.10 E86Q the mutant retains catalytic proficiency and is optimally active at wild type conditions. The E86Q mutation rather modestly affects kcat, decreasing it by a factor of 4 Salmonella enterica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.10 0.03
-
 3-dehydroquinate mutant enzyme E86Q, at pH 7.5 and 37°C Salmonella enterica
4.2.1.10 0.046
-
 3-dehydroquinate mutant enzyme E86A, at pH 7.5 and 37°C Salmonella enterica
4.2.1.10 0.053
-
 3-dehydroquinate wild type enzyme, at pH 7.5 and 37°C Salmonella enterica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.10 3-dehydroquinate Salmonella enterica
-
 3-dehydroshikimate + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.10 Salmonella enterica P58687
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.10
-
 Salmonella enterica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.10 3-dehydroquinate
-
 Salmonella enterica 3-dehydroshikimate + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.10 DHQD
-
 Salmonella enterica
4.2.1.10 type I dehydroquinate dehydratase
-
 Salmonella enterica

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.10 18
-
 3-dehydroquinate mutant enzyme E86A, at pH 7.5 and 37°C Salmonella enterica
4.2.1.10 78
-
 3-dehydroquinate mutant enzyme E86Q, at pH 7.5 and 37°C Salmonella enterica
4.2.1.10 310
-
 3-dehydroquinate wild type enzyme, at pH 7.5 and 37°C Salmonella enterica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.10 7.5
-
-
 Salmonella enterica

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.1.10 400
-
 3-dehydroquinate mutant enzyme E86A, at pH 7.5 and 37°C Salmonella enterica
4.2.1.10 2600
-
 3-dehydroquinate mutant enzyme E86Q, at pH 7.5 and 37°C Salmonella enterica
4.2.1.10 5800
-
 3-dehydroquinate wild type enzyme, at pH 7.5 and 37°C Salmonella enterica