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Literature summary extracted from

  • Kant, S.; Vohra, A.; Gupta, R.
    Purification and physicochemical properties of polygalacturonase from Aspergillus niger MTCC 3323 (2013), Protein Expr. Purif., 87, 11-16.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.15 food industry the enzyme is used for guava juice extraction and clarification. The recovery of juice of enzymatically treated pulp increases from 6% to 23%. Addition of purified enzyme increases the%T650 from 2.5 to 20.4 and °Brix from 1.9 to 4.8. The pH of the enzyme treated juice decreases from 4.5 to 3.02 Aspergillus niger
3.2.1.67 food industry the enzyme is used for guava juice extraction and clarification. The recovery of juice of enzymatically treated pulp increases from 6% to 23%. Addition of purified enzyme increases the%T650 from 2.5 to 20.4 and °Brix from 1.9 to 4.8. The pH of the enzyme treated juice decreases from 4.5 to 3.02 Aspergillus niger

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.15 Al3+
-
 Aspergillus niger
3.2.1.15 ascorbic acid
-
 Aspergillus niger
3.2.1.15 Ca2+
-
 Aspergillus niger
3.2.1.15 chlorogenic acid
-
 Aspergillus niger
3.2.1.15 Cinnamic acid
-
 Aspergillus niger
3.2.1.15 Fe2+
-
 Aspergillus niger
3.2.1.15 ferulic acid
-
 Aspergillus niger
3.2.1.15 K+
-
 Aspergillus niger
3.2.1.15 L-cystine
-
 Aspergillus niger
3.2.1.15 Mercuric chloride
-
 Aspergillus niger
3.2.1.15 Mn2+
-
 Aspergillus niger
3.2.1.15 additional information inhibitory potency in descending order cinnamic acid, ferulic acid, p-coumaric acid, salicylic acid, and chlorgenic acid Aspergillus niger
3.2.1.15 p-coumaric acid
-
 Aspergillus niger
3.2.1.15 salicylic acid
-
 Aspergillus niger
3.2.1.15 Zn2+
-
 Aspergillus niger
3.2.1.67 Al3+
-
 Aspergillus niger
3.2.1.67 Ca2+
-
 Aspergillus niger
3.2.1.67 Fe2+
-
 Aspergillus niger
3.2.1.67 K+
-
 Aspergillus niger
3.2.1.67 Mn2+
-
 Aspergillus niger
3.2.1.67 Zn2+
-
 Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.15 additional information
-
 additional information 0.083 mg/ml with polygalacturonic acid at pH 4.8, 45°C Aspergillus niger
3.2.1.67 additional information
-
 additional information 0.083 mg/ml with polygalacturonic acid at pH 4.8, 45°C Aspergillus niger

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.15 Cu2+ activates Aspergillus niger
3.2.1.15 Mg2+ activates Aspergillus niger
3.2.1.67 Cu2+ activates Aspergillus niger
3.2.1.67 Mg2+ activates Aspergillus niger

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.15 34000
-
 1 * 34000 + 1 * 69000, SDS-PAGE Aspergillus niger
3.2.1.15 69000
-
 1 * 34000 + 1 * 69000, SDS-PAGE Aspergillus niger
3.2.1.15 106000
-
 native PAGE Aspergillus niger
3.2.1.67 34000
-
 1 * 34000 + 1 * 69000, SDS-PAGE Aspergillus niger
3.2.1.67 69000
-
 1 * 34000 + 1 * 69000, SDS-PAGE Aspergillus niger
3.2.1.67 106000
-
 native PAGE Aspergillus niger

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.15 additional information Aspergillus niger the enzyme catalyzes the hydrolytic cleavage of the polygalacturonic acid chain ?
-
 ?
3.2.1.15 additional information Aspergillus niger MTCC 3323 the enzyme catalyzes the hydrolytic cleavage of the polygalacturonic acid chain ?
-
 ?
3.2.1.67 additional information Aspergillus niger the enzyme catalyzes the hydrolytic cleavage of the polygalacturonic acid chain ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.15 Aspergillus niger
-
-
-
3.2.1.15 Aspergillus niger MTCC 3323
-
-
-
3.2.1.67 Aspergillus niger
-
-
-
3.2.1.67 Aspergillus niger MTCC 3323
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.15 native enzyme 6.5fold by ethanol precipitation and gel filtration to homogeneity Aspergillus niger
3.2.1.67 native enzyme 6.5fold by ethanol precipitation and gel filtration to homogeneity Aspergillus niger

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.15 54.3
-
 determined as micromol/ml/mg protein, purified enzyme, pH 4.8, 45°C, substrate polygalacturonic acid Aspergillus niger
3.2.1.67 54.3
-
 purified enzyme, pH 4.8, 45°C, substrate polygalacturonic acid Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.15 amylopectin + H2O
-
 Aspergillus niger ?
-
 ?
3.2.1.15 amylopectin + H2O
-
 Aspergillus niger MTCC 3323 ?
-
 ?
3.2.1.15 dextrose agar + H2O potato dextrose agar, low activity Aspergillus niger ?
-
 ?
3.2.1.15 dextrose agar + H2O potato dextrose agar, low activity Aspergillus niger MTCC 3323 ?
-
 ?
3.2.1.15 additional information the enzyme catalyzes the hydrolytic cleavage of the polygalacturonic acid chain Aspergillus niger ?
-
 ?
3.2.1.15 additional information the enzyme catalyzes the hydrolytic cleavage of the polygalacturonic acid chain Aspergillus niger MTCC 3323 ?
-
 ?
3.2.1.15 pectin + H2O citrus or apple pectin Aspergillus niger ?
-
 ?
3.2.1.15 pectin + H2O citrus or apple pectin Aspergillus niger MTCC 3323 ?
-
 ?
3.2.1.15 polygalacturonic acid + H2O
-
 Aspergillus niger galacturonic acid
-
 ?
3.2.1.15 polygalacturonic acid + H2O
-
 Aspergillus niger MTCC 3323 galacturonic acid
-
 ?
3.2.1.67 amylopectin + H2O
-
 Aspergillus niger ?
-
 ?
3.2.1.67 dextrose agar + H2O potato dextrose agar, low activity Aspergillus niger ?
-
 ?
3.2.1.67 additional information the enzyme catalyzes the hydrolytic cleavage of the polygalacturonic acid chain Aspergillus niger ?
-
 ?
3.2.1.67 pectin + H2O citrus or apple pectin Aspergillus niger ?
-
 ?
3.2.1.67 polygalacturonic acid + H2O
-
 Aspergillus niger galacturonic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.15 heterodimer 1 * 34000 + 1 * 69000, SDS-PAGE Aspergillus niger
3.2.1.67 heterodimer 1 * 34000 + 1 * 69000, SDS-PAGE Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.15 45
-
-
 Aspergillus niger
3.2.1.67 45
-
-
 Aspergillus niger

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.15 4
-
 purified enzyme, half-life is 104 h Aspergillus niger
3.2.1.15 25
-
 purified enzyme, half-life is 3 h Aspergillus niger
3.2.1.67 4
-
 purified enzyme, half-life is 104 h Aspergillus niger
3.2.1.67 25
-
 purified enzyme, half-life is 3 h Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.15 4.8
-
-
 Aspergillus niger
3.2.1.67 4.8
-
-
 Aspergillus niger

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.15 3.5 6.5 over 50% of maximal acitivity at pH 3.5-6.0, 32% at pH 6.5 Aspergillus niger
3.2.1.67 3.5 6.5 over 50% of maximal acitivity at pH 3.5-6.0, 32% at pH 6.5 Aspergillus niger

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.15 4 5.5 purified enzyme, 1 h, stable at Aspergillus niger
3.2.1.67 4 5.5 purified enzyme, 1 h, stable at Aspergillus niger