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Literature summary extracted from

  • Rinagelová, A.; Kaplan, O.; Vesela, A.; Chmatal, M.; Krenkova, A.; Plihal, O.; Pasquarelli, F.; Cantarella, M.; Martinková, L.
    Cyanide hydratase from Aspergillus niger K10: Overproduction in Escherichia coli, purification, characterization and use in continuous cyanide degradation (2014), Process Biochem., 49, 445-450.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.66 expressed in Escherichia coli BL21-Gold(DE3) cells Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.66 3.7
-
 2-Cyanopyridine at pH 8.0 and 30°C Aspergillus niger
4.2.1.66 14.7
-
 Fumaronitrile at pH 8.0 and 30°C Aspergillus niger
4.2.1.66 109
-
 cyanide at pH 8.0 and 30°C Aspergillus niger

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.66 40000
-
 x * 40000, SDS-PAGE Aspergillus niger

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.66 cyanide + H2O Aspergillus niger 100% activity formamide
-
 ?
4.2.1.66 cyanide + H2O Aspergillus niger K-10 100% activity formamide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.66 Aspergillus niger
-
-
-
4.2.1.66 Aspergillus niger K-10
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.66 Hi-Prep 16/10 Q FF column chromatography and Sephacryl S-200 gel filtration Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.66 2-cyanopyridine + 2 H2O 0.76% activity compared to cyanide Aspergillus niger pyridine 2-carboxylic acid + NH3
-
 ?
4.2.1.66 2-cyanopyridine + 2 H2O 0.76% activity compared to cyanide Aspergillus niger K-10 pyridine 2-carboxylic acid + NH3
-
 ?
4.2.1.66 cyanide + H2O 100% activity Aspergillus niger formamide
-
 ?
4.2.1.66 cyanide + H2O 100% activity Aspergillus niger K-10 formamide
-
 ?
4.2.1.66 fumaronitrile + H2O 0.95% activity compared to cyanide Aspergillus niger ?
-
 ?
4.2.1.66 fumaronitrile + H2O 0.95% activity compared to cyanide Aspergillus niger K-10 ?
-
 ?
4.2.1.66 additional information almost no activity with benzonitrile, 3-cyanopyridine, 4-cyanopyridine, and phenylacetonitrile Aspergillus niger ?
-
 ?
4.2.1.66 additional information almost no activity with benzonitrile, 3-cyanopyridine, 4-cyanopyridine, and phenylacetonitrile Aspergillus niger K-10 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.66 ? x * 40000, SDS-PAGE Aspergillus niger

Synonyms

EC Number Synonyms Comment Organism
4.2.1.66 CHT
-
 Aspergillus niger
4.2.1.66 NitAn1
-
 Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.66 45
-
-
 Aspergillus niger

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.66 28 55 the enzyme shows a half-life of 5.6 hat 28°C and is unstable at temperatures above 35°C. The enzyme is still active at 50°C with ca. 36% of its maximum activity, but nearly inactive at 55°C Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.66 8 9
-
 Aspergillus niger

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.1.66 5.5 10.5
-
 Aspergillus niger

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.2.1.66 5.5 10 unstable below pH 5.5 Aspergillus niger