EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.5.1.1 | 0.037 | - |
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane | pH 7.0, 37°C | Pseudomonas putida |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.5.1.1 | Ca2+ | a metalloprotein containing Zn2+, Mg2+, and Ca2+ ions | Pseudomonas putida | |
4.5.1.1 | Mg2+ | a metalloprotein containing Zn2+, Mg2+, and Ca2+ ions | Pseudomonas putida | |
4.5.1.1 | Zn2+ | a metalloprotein containing Zn2+, Mg2+, and Ca2+ ions | Pseudomonas putida |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.5.1.1 | 37000 | - |
about, native PAGE | Pseudomonas putida |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.5.1.1 | 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane | Pseudomonas putida | - |
1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene + chloride | - |
? | |
4.5.1.1 | 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane | Pseudomonas putida T5 | - |
1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene + chloride | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.5.1.1 | Pseudomonas putida | - |
- |
- |
4.5.1.1 | Pseudomonas putida T5 | - |
- |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
4.5.1.1 | glycoprotein | a glycoprotein with mannose forming the backbone | Pseudomonas putida |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.5.1.1 | native enzyme from strain T5 to homogeneity | Pseudomonas putida |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.5.1.1 | 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane | - |
Pseudomonas putida | 1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene + chloride | - |
? | |
4.5.1.1 | 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane | - |
Pseudomonas putida T5 | 1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene + chloride | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.5.1.1 | monomer | 1 * 37000, SDS-PAGE | Pseudomonas putida |
4.5.1.1 | More | mass spectrometric sequence canalysis | Pseudomonas putida |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.5.1.1 | DDT-dehydrohalogenase | - |
Pseudomonas putida |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.5.1.1 | 37 | - |
- |
Pseudomonas putida |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.5.1.1 | 37 | - |
purified native enzyme, pH 7.0, stable for 4-5 h | Pseudomonas putida |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.5.1.1 | 0.113 | - |
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane | pH 7.0, 37°C | Pseudomonas putida |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.5.1.1 | 7 | - |
- |
Pseudomonas putida |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
4.5.1.1 | 7 | - |
purified native enzyme, 37°C, stable for 4-5 h | Pseudomonas putida |
EC Number | General Information | Comment | Organism |
---|---|---|---|
4.5.1.1 | additional information | serine and tryptophan appear to be involved at the active site | Pseudomonas putida |
4.5.1.1 | physiological function | the enzyme is involved in the catalytic degradation of 4,4'-DDT by eliminating either HCl or Cl- to form DDD/DDE | Pseudomonas putida |