Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Gruninger, R.J.; Thibault, J.; Capeness, M.J.; Till, R.; Mosimann, S.C.; Sockett, R.E.; Selinger, B.L.; Lovering, A.L.
    Structural and biochemical analysis of a unique phosphatase from Bdellovibrio bacteriovorus reveals its structural and functional relationship with the protein tyrosine phosphatase class of phytase (2014), PLoS ONE, 9, e94403.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.48 expressed in Escherichia coli BL21(DE3) cells Bdellovibrio bacteriovorus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.3.48 hanging drop vapor diffusion method, using 200 mM MgCl2, 100 mM Tris-HCl (pH 7.0), 8-10% (w/v) PEG 8000 Bdellovibrio bacteriovorus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.48 0.574
-
 Inositol hexakisphosphate in 2 mM phytate in 50 mM sodium acetate pH 4.0, at 50°C Bdellovibrio bacteriovorus

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.48 Bdellovibrio bacteriovorus Q6MNP0
-
-
3.1.3.48 Bdellovibrio bacteriovorus HD100 Q6MNP0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.48 HiTrap Ni-IMAC column chromatography and Superdex 200 gel filtration Bdellovibrio bacteriovorus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.48 0.041
-
 using O-phospho-L-tyrosine as substrate, in 2 mM phytate in 50 mM sodium acetate pH 4.0, at 50°C Bdellovibrio bacteriovorus
3.1.3.48 0.073
-
 using ADP as substrate, in 2 mM phytate in 50 mM sodium acetate pH 4.0, at 50°C Bdellovibrio bacteriovorus
3.1.3.48 0.093
-
 using phenolphthalein diphosphate as substrate, in 2 mM phytate in 50 mM sodium acetate pH 4.0, at 50°C Bdellovibrio bacteriovorus
3.1.3.48 0.14
-
 using 4-nitrophenyl phosphate as substrate, in 2 mM phytate in 50 mM sodium acetate pH 4.0, at 50°C Bdellovibrio bacteriovorus
3.1.3.48 0.2
-
 using 1-naphthyl phosphate as substrate, in 2 mM phytate in 50 mM sodium acetate pH 4.0, at 50°C Bdellovibrio bacteriovorus
3.1.3.48 0.36
-
 using D-fructose 1,6-diphosphate as substrate, in 2 mM phytate in 50 mM sodium acetate pH 4.0, at 50°C Bdellovibrio bacteriovorus
3.1.3.48 1.65
-
 using ATP as substrate, in 2 mM phytate in 50 mM sodium acetate pH 4.0, at 50°C Bdellovibrio bacteriovorus
3.1.3.48 310.8
-
 using inositol hexakisphosphate as substrate, in 2 mM phytate in 50 mM sodium acetate pH 4.0, at 50°C Bdellovibrio bacteriovorus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.48 1-naphthyl phosphate + H2O
-
 Bdellovibrio bacteriovorus ?
-
 ?
3.1.3.48 4-nitrophenyl phosphate + H2O
-
 Bdellovibrio bacteriovorus 4-nitrophenol + phosphate
-
 ?
3.1.3.48 4-nitrophenyl phosphate + H2O
-
 Bdellovibrio bacteriovorus HD100 4-nitrophenol + phosphate
-
 ?
3.1.3.48 ADP + H2O
-
 Bdellovibrio bacteriovorus ?
-
 ?
3.1.3.48 ADP + H2O
-
 Bdellovibrio bacteriovorus HD100 ?
-
 ?
3.1.3.48 ATP + H2O
-
 Bdellovibrio bacteriovorus ?
-
 ?
3.1.3.48 ATP + H2O
-
 Bdellovibrio bacteriovorus HD100 ?
-
 ?
3.1.3.48 D-fructose 1,6-diphosphate + H2O
-
 Bdellovibrio bacteriovorus ?
-
 ?
3.1.3.48 D-fructose 1,6-diphosphate + H2O
-
 Bdellovibrio bacteriovorus HD100 ?
-
 ?
3.1.3.48 inositol hexakisphosphate + H2O highest activity Bdellovibrio bacteriovorus ?
-
 ?
3.1.3.48 additional information the enzyme does not display catalytic activity against some common protein tyrosine phosphatase substrates (O-nitrophenyl beta-D-galactopyranoside, O-phospho-L-threonine, D-fructose 6-phosphate, D-ribose 5-phosphate, D-glucose 6-phosphate, D-glucose 1-phosphate) but is highly specific for hydrolysis of phosphomonoester bonds of inositol hexakisphosphate Bdellovibrio bacteriovorus ?
-
 ?
3.1.3.48 additional information the enzyme does not display catalytic activity against some common protein tyrosine phosphatase substrates (O-nitrophenyl beta-D-galactopyranoside, O-phospho-L-threonine, D-fructose 6-phosphate, D-ribose 5-phosphate, D-glucose 6-phosphate, D-glucose 1-phosphate) but is highly specific for hydrolysis of phosphomonoester bonds of inositol hexakisphosphate Bdellovibrio bacteriovorus HD100 ?
-
 ?
3.1.3.48 O-phospho-L-tyrosine + H2O
-
 Bdellovibrio bacteriovorus ?
-
 ?
3.1.3.48 phenolphthalein diphosphate + H2O
-
 Bdellovibrio bacteriovorus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.48 Bd1204
-
 Bdellovibrio bacteriovorus
3.1.3.48 protein tyrosine phosphatase
-
 Bdellovibrio bacteriovorus
3.1.3.48 PTP
-
 Bdellovibrio bacteriovorus
3.1.3.48 PTP-like phytase
-
 Bdellovibrio bacteriovorus
3.1.3.48 PTPLP
-
 Bdellovibrio bacteriovorus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.48 50
-
-
 Bdellovibrio bacteriovorus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.3.48 40 55 more than 60% activity Bdellovibrio bacteriovorus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.48 124
-
 Inositol hexakisphosphate in 2 mM phytate in 50 mM sodium acetate pH 4.0, at 50°C Bdellovibrio bacteriovorus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.48 4
-
-
 Bdellovibrio bacteriovorus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.48 3.5 5 more than 40% activity Bdellovibrio bacteriovorus