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Literature summary extracted from

  • Guan, Z.; Liu, J.; Bai, W.; Lv, Z.; Jiang, X.; Yang, S.; Chen, A.; Lv, G.
    Label-free and sensitive fluorescent detection of sequence-specific single-strand DNA based on S1 nuclease cleavage effects (2014), PLoS ONE, 9, e108401.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.1.30.1 analysis development of a fluorescent method for simple detection of sequence-specific single-strand DNA in complex, contaminant-ridden samples directly without a DNA extraction and PCR step could simplify the detection of pathogens in the field and in the clinic. Upon clearing away redundant complementary ssDNA and possibly mismatched double strand DNA by using S1 nuclease, the fluorescent signal-to-noise ratio can be increased dramatically. It enables the method to be adaptable to three different types of DNA fluorescent dyes and the ability to detect target ssDNA in complex, multicomponent samples, like tissue homogenate from pork Aspergillus oryzae

Organism

EC Number Organism UniProt Comment Textmining
3.1.30.1 Aspergillus oryzae
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.30.1 commercial preparation
-
 Aspergillus oryzae
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Synonyms

EC Number Synonyms Comment Organism
3.1.30.1 S1 nuclease
-
 Aspergillus oryzae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.30.1 22
-
 assay at room temperature Aspergillus oryzae