Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Lajoie, D.M.; Zobel-Thropp, P.A.; Kumirov, V.K.; Bandarian, V.; Binford, G.J.; Cordes, M.H.
    Phospholipase D toxins of brown spider venom convert lysophosphatidylcholine and sphingomyelin to cyclic phosphates (2013), PLoS ONE, 8, e72372.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.41 recombinant expression of N-terminally His-tagged recombinant enzyme in Escherichia coli strain BL21(lDE3) Loxosceles reclusa
3.1.4.41 recombinant expression of N-terminally His-tagged recombinant enzyme in Escherichia coli strain BL21(lDE3) Loxosceles laeta
3.1.4.41 recombinant expression of N-terminally His-tagged recombinant enzyme in Escherichia coli strain BL21(lDE3) Loxosceles arizonica

Protein Variants

EC Number Protein Variants Comment Organism
3.1.4.41 H47N inactive mutant Loxosceles reclusa
3.1.4.41 H47N inactive mutant Loxosceles laeta
3.1.4.41 H47N inactive mutant Loxosceles arizonica

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.41 extracellular
-
 Loxosceles reclusa
-
-
3.1.4.41 extracellular
-
 Loxosceles laeta
-
-
3.1.4.41 extracellular
-
 Loxosceles arizonica
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.41 2-lysophosphatidylcholine + H2O Loxosceles reclusa
-
 2-lysophosphatidate + choline
-
 ?
3.1.4.41 2-lysophosphatidylcholine + H2O Loxosceles laeta
-
 2-lysophosphatidate + choline
-
 ?
3.1.4.41 2-lysophosphatidylcholine + H2O Loxosceles arizonica
-
 2-lysophosphatidate + choline
-
 ?
3.1.4.41 additional information Loxosceles reclusa the spider venom enzyme cleaves the substrates sphingomyelin and lysophosphatidylcholine in mammalian tissues, releasing the choline head group ?
-
 ?
3.1.4.41 additional information Loxosceles laeta the spider venom enzyme cleaves the substrates sphingomyelin and lysophosphatidylcholine in mammalian tissues, releasing the choline head group ?
-
 ?
3.1.4.41 additional information Loxosceles arizonica the spider venom enzyme cleaves the substrates sphingomyelin and lysophosphatidylcholine in mammalian tissues, releasing the choline head group ?
-
 ?
3.1.4.41 sphingomyelin + H2O Loxosceles reclusa
-
 ceramide phosphate + choline
-
 ?
3.1.4.41 sphingomyelin + H2O Loxosceles laeta
-
 ceramide phosphate + choline
-
 ?
3.1.4.41 sphingomyelin + H2O Loxosceles arizonica
-
 ceramide phosphate + choline
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.41 Loxosceles arizonica Q4ZFU2
-
-
3.1.4.41 Loxosceles laeta
-
-
-
3.1.4.41 Loxosceles reclusa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.41 recombinant N-terminally His-tagged recombinant enzyme from Escherichia coli strain BL21(lDE3) by nickel affinity chromatography Loxosceles reclusa
3.1.4.41 recombinant N-terminally His-tagged recombinant enzyme from Escherichia coli strain BL21(lDE3) by nickel affinity chromatography Loxosceles laeta
3.1.4.41 recombinant N-terminally His-tagged recombinant enzyme from Escherichia coli strain BL21(lDE3) by nickel affinity chromatography Loxosceles arizonica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.41 venom
-
 Loxosceles reclusa
-
3.1.4.41 venom
-
 Loxosceles laeta
-
3.1.4.41 venom
-
 Loxosceles arizonica
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.41 1-octanoyl-2-hydroxy-sn-glycero-3-phosphocholine + H2O Loxosceles whole venoms cleanly convert 8:0 LPC substrate to 8:0 CPA product Loxosceles reclusa 1-octanoyl-sn-glycero-2,3-cyclic-phosphate + choline
-
 ?
3.1.4.41 1-octanoyl-2-hydroxy-sn-glycero-3-phosphocholine + H2O Loxosceles whole venoms cleanly convert 8:0 LPC substrate to 8:0 CPA product Loxosceles laeta 1-octanoyl-sn-glycero-2,3-cyclic-phosphate + choline
-
 ?
3.1.4.41 1-octanoyl-2-hydroxy-sn-glycero-3-phosphocholine + H2O Loxosceles whole venoms cleanly convert 8:0 LPC substrate to 8:0 CPA product Loxosceles arizonica 1-octanoyl-sn-glycero-2,3-cyclic-phosphate + choline
-
 ?
3.1.4.41 1-palmitoyl-2-hydroxy-sn-glycero-3-phosphocholine + H2O Loxosceles whole venoms and recombinant enzyme Loxosceles reclusa 1-palmitoyl-sn-glycero-2,3-cyclic-phosphate + choline
-
 ?
3.1.4.41 1-palmitoyl-2-hydroxy-sn-glycero-3-phosphocholine + H2O Loxosceles whole venoms and recombinant enzyme Loxosceles laeta 1-palmitoyl-sn-glycero-2,3-cyclic-phosphate + choline
-
 ?
3.1.4.41 1-palmitoyl-2-hydroxy-sn-glycero-3-phosphocholine + H2O Loxosceles whole venoms and recombinant enzyme Loxosceles arizonica 1-palmitoyl-sn-glycero-2,3-cyclic-phosphate + choline
-
 ?
3.1.4.41 2-lysophosphatidylcholine + H2O
-
 Loxosceles reclusa 2-lysophosphatidate + choline
-
 ?
3.1.4.41 2-lysophosphatidylcholine + H2O
-
 Loxosceles laeta 2-lysophosphatidate + choline
-
 ?
3.1.4.41 2-lysophosphatidylcholine + H2O
-
 Loxosceles arizonica 2-lysophosphatidate + choline
-
 ?
3.1.4.41 additional information the spider venom enzyme cleaves the substrates sphingomyelin and lysophosphatidylcholine in mammalian tissues, releasing the choline head group Loxosceles reclusa ?
-
 ?
3.1.4.41 additional information the spider venom enzyme cleaves the substrates sphingomyelin and lysophosphatidylcholine in mammalian tissues, releasing the choline head group Loxosceles laeta ?
-
 ?
3.1.4.41 additional information the spider venom enzyme cleaves the substrates sphingomyelin and lysophosphatidylcholine in mammalian tissues, releasing the choline head group Loxosceles arizonica ?
-
 ?
3.1.4.41 additional information recombinant enzymes, as well as whole venoms from diverse Loxosceles species, exclusively catalyze transphosphatidylation rather than hydrolysis, forming cyclic phosphate products from both major substrates, e.g. 1-palmitoyl-sn-glycero-2,3-cyclic-phosphate. Cyclic phosphates have vastly different biological properties from their monoester counterparts, and they may be relevant to the pathology of brown spider envenomation Loxosceles reclusa ?
-
 ?
3.1.4.41 additional information recombinant enzymes, as well as whole venoms from diverse Loxosceles species, exclusively catalyze transphosphatidylation rather than hydrolysis, forming cyclic phosphate products from both major substrates, e.g. 1-palmitoyl-sn-glycero-2,3-cyclic-phosphate. Cyclic phosphates have vastly different biological properties from their monoester counterparts, and they may be relevant to the pathology of brown spider envenomation Loxosceles laeta ?
-
 ?
3.1.4.41 additional information recombinant enzymes, as well as whole venoms from diverse Loxosceles species, exclusively catalyze transphosphatidylation rather than hydrolysis, forming cyclic phosphate products from both major substrates, e.g. 1-palmitoyl-sn-glycero-2,3-cyclic-phosphate. Cyclic phosphates have vastly different biological properties from their monoester counterparts, and they may be relevant to the pathology of brown spider envenomation Loxosceles arizonica ?
-
 ?
3.1.4.41 sphingomyelin + H2O
-
 Loxosceles reclusa ceramide phosphate + choline
-
 ?
3.1.4.41 sphingomyelin + H2O
-
 Loxosceles laeta ceramide phosphate + choline
-
 ?
3.1.4.41 sphingomyelin + H2O
-
 Loxosceles arizonica ceramide phosphate + choline
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.4.41 A1LB1
-
 Loxosceles arizonica
3.1.4.41 alphaIB2bi
-
 Loxosceles arizonica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.4.41 37
-
 assay at Loxosceles reclusa
3.1.4.41 37
-
 assay at Loxosceles laeta
3.1.4.41 37
-
 assay at Loxosceles arizonica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.41 8
-
 assay at Loxosceles reclusa
3.1.4.41 8
-
 assay at Loxosceles laeta
3.1.4.41 8
-
 assay at Loxosceles arizonica