EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.3.8 | - |
Hafnia alvei |
3.1.3.8 | - |
Yersinia kristensenii |
3.1.3.26 | - |
Hafnia alvei |
3.1.3.26 | - |
Yersinia kristensenii |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.1.3.8 | apo-enzyme, to 1.9 A resolution, and in complex with substrate analogue myo-inositiol hexakissulfate, to 1.59 A resolution. The substrate analogue is coordinated by residues Arg18, His19, Arg22, Thr25, Lys26, His128, Thr219, His306, Asp307 and a number of water molecules. The Nepsilon2 atom of the catalytic nucleophile, His19, lies 3.2 A from S6 of the analogue and is in-line with the S-O bond from the inositol ring. The OD1 atom of Asp307, the proton donor, forms an H-bond with the oxygen at position 6 of the inositol ring | Hafnia alvei |
3.1.3.8 | in complex with phosphate, to 1.9 A resolution | Yersinia kristensenii |
3.1.3.26 | apo-enzyme, to 1.9 A resolution, and in complex with substrate analogue myo-inositiol hexakissulfate, to 1.59 A resolution. Te substrate analogue is coordinated by residues Arg18, His19, Arg22, Thr25, Lys26, His128, Thr219, His306, Asp307 and a number of water molecules. The Nepsilon2 atom of the catalytic nucleophile, His19, lies 3.2 A from S6 of the analogue and is in-line with the S-O bond from the inositol ring. The OD1 atom of Asp307, the proton donor, forms an H-bond with the oxygen at position 6 of the inositol ring | Hafnia alvei |
3.1.3.26 | in complex with phosphate, to 1.9 A resolution | Yersinia kristensenii |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.1.3.8 | T308A | 5% of the specific activity of wild-type | Hafnia alvei |
3.1.3.26 | T308A | 5% of the specific activity of wild-type | Hafnia alvei |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.3.8 | Hafnia alvei | H9TUK5 | - |
- |
3.1.3.8 | Yersinia kristensenii | H9TUK6 | - |
- |
3.1.3.26 | Hafnia alvei | H9TUK5 | - |
- |
3.1.3.26 | Yersinia kristensenii | H9TUK6 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.3.8 | recombinant protein | Hafnia alvei |
3.1.3.8 | recombinant protein | Yersinia kristensenii |
3.1.3.26 | recombinant protein | Hafnia alvei |
3.1.3.26 | recombinant protein | Yersinia kristensenii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.8 | additional information | enzyme is a 6-phytase, EC 3.1.3.26. Major degradation product is DL-inositol 1,2,3,4,5-pentaphosphate | Hafnia alvei | ? | - |
? | |
3.1.3.8 | additional information | enzyme is a 6-phytase, EC 3.1.3.26. Major degradation product is DL-inositol 1,2,3,4,5-pentaphosphate | Yersinia kristensenii | ? | - |
? | |
3.1.3.8 | myo-inositol hexakisphosphate + H2O | - |
Hafnia alvei | DL-inositol 1,2,4,5,6-pentaphosphate + phosphate | 10-14% of product | ? | |
3.1.3.8 | myo-inositol hexakisphosphate + H2O | - |
Yersinia kristensenii | DL-inositol 1,2,4,5,6-pentaphosphate + phosphate | 10-14% of product | ? | |
3.1.3.26 | myo-inositol hexakisphosphate + H2O | - |
Hafnia alvei | DL-inositol 1,2,3,4,5-pentaphosphate + phosphate | major degradation product | ? | |
3.1.3.26 | myo-inositol hexakisphosphate + H2O | - |
Yersinia kristensenii | DL-inositol 1,2,3,4,5-pentaphosphate + phosphate | major degradation product, plus DL-inositol 1,2,4,5,6-pentaphosphate, DL-inositol 1,2,3,4-tetraphosphate and DL-inositol 1,2,4,6-tetraphosphate | ? | |
3.1.3.26 | myo-inositol hexakisphosphate + H2O | - |
Hafnia alvei | DL-inositol 1,2,4,5,6-pentaphosphate + phosphate | activity of EC 3.1.3.8, 10-14% of product | ? | |
3.1.3.26 | myo-inositol hexakisphosphate + H2O | - |
Yersinia kristensenii | DL-inositol 1,2,4,5,6-pentaphosphate + phosphate | activity of EC 3.1.3.8, 10-14% of product | ? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.3.8 | PhyA | - |
Hafnia alvei |
3.1.3.8 | PhyA | - |
Yersinia kristensenii |
3.1.3.26 | PhyA | - |
Hafnia alvei |
3.1.3.26 | PhyA | - |
Yersinia kristensenii |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.3.8 | 55 | - |
- |
Yersinia kristensenii |
3.1.3.8 | 65 | - |
- |
Hafnia alvei |
3.1.3.26 | 55 | - |
- |
Yersinia kristensenii |
3.1.3.26 | 65 | - |
- |
Hafnia alvei |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.3.8 | 57 | - |
melting temperature | Yersinia kristensenii |
3.1.3.8 | 70 | - |
melting temperature | Hafnia alvei |
3.1.3.26 | 57 | - |
melting temperature | Yersinia kristensenii |
3.1.3.26 | 70 | - |
melting temperature | Hafnia alvei |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.3.8 | 4 | 4.5 | - |
Hafnia alvei |
3.1.3.8 | 4 | 4.5 | - |
Yersinia kristensenii |
3.1.3.26 | 4 | 4.5 | - |
Hafnia alvei |
3.1.3.26 | 4 | 4.5 | - |
Yersinia kristensenii |