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Literature summary extracted from

  • Ariza, A.; Moroz, O.V.; Blagova, E.V.; Turkenburg, J.P.; Waterman, J.; Roberts, S.M.; Vind, J.; Sj?holm, C.; Lassen, S.F.; De Maria, L.; Glitsoe, V.; Skov, L.K.; Wilson, K.S.
    Degradation of phytate by the 6-phytase from Hafnia alvei: a combined structural and solution study (2013), PLoS ONE, 8, e65062.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.8
-
Hafnia alvei
3.1.3.8
-
Yersinia kristensenii
3.1.3.26
-
Hafnia alvei
3.1.3.26
-
Yersinia kristensenii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.3.8 apo-enzyme, to 1.9 A resolution, and in complex with substrate analogue myo-inositiol hexakissulfate, to 1.59 A resolution. The substrate analogue is coordinated by residues Arg18, His19, Arg22, Thr25, Lys26, His128, Thr219, His306, Asp307 and a number of water molecules. The Nepsilon2 atom of the catalytic nucleophile, His19, lies 3.2 A from S6 of the analogue and is in-line with the S-O bond from the inositol ring. The OD1 atom of Asp307, the proton donor, forms an H-bond with the oxygen at position 6 of the inositol ring Hafnia alvei
3.1.3.8 in complex with phosphate, to 1.9 A resolution Yersinia kristensenii
3.1.3.26 apo-enzyme, to 1.9 A resolution, and in complex with substrate analogue myo-inositiol hexakissulfate, to 1.59 A resolution. Te substrate analogue is coordinated by residues Arg18, His19, Arg22, Thr25, Lys26, His128, Thr219, His306, Asp307 and a number of water molecules. The Nepsilon2 atom of the catalytic nucleophile, His19, lies 3.2 A from S6 of the analogue and is in-line with the S-O bond from the inositol ring. The OD1 atom of Asp307, the proton donor, forms an H-bond with the oxygen at position 6 of the inositol ring Hafnia alvei
3.1.3.26 in complex with phosphate, to 1.9 A resolution Yersinia kristensenii

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.8 T308A 5% of the specific activity of wild-type Hafnia alvei
3.1.3.26 T308A 5% of the specific activity of wild-type Hafnia alvei

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.8 Hafnia alvei H9TUK5
-
-
3.1.3.8 Yersinia kristensenii H9TUK6
-
-
3.1.3.26 Hafnia alvei H9TUK5
-
-
3.1.3.26 Yersinia kristensenii H9TUK6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.8 recombinant protein Hafnia alvei
3.1.3.8 recombinant protein Yersinia kristensenii
3.1.3.26 recombinant protein Hafnia alvei
3.1.3.26 recombinant protein Yersinia kristensenii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.8 additional information enzyme is a 6-phytase, EC 3.1.3.26. Major degradation product is DL-inositol 1,2,3,4,5-pentaphosphate Hafnia alvei ?
-
?
3.1.3.8 additional information enzyme is a 6-phytase, EC 3.1.3.26. Major degradation product is DL-inositol 1,2,3,4,5-pentaphosphate Yersinia kristensenii ?
-
?
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
Hafnia alvei DL-inositol 1,2,4,5,6-pentaphosphate + phosphate 10-14% of product ?
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
Yersinia kristensenii DL-inositol 1,2,4,5,6-pentaphosphate + phosphate 10-14% of product ?
3.1.3.26 myo-inositol hexakisphosphate + H2O
-
Hafnia alvei DL-inositol 1,2,3,4,5-pentaphosphate + phosphate major degradation product ?
3.1.3.26 myo-inositol hexakisphosphate + H2O
-
Yersinia kristensenii DL-inositol 1,2,3,4,5-pentaphosphate + phosphate major degradation product, plus DL-inositol 1,2,4,5,6-pentaphosphate, DL-inositol 1,2,3,4-tetraphosphate and DL-inositol 1,2,4,6-tetraphosphate ?
3.1.3.26 myo-inositol hexakisphosphate + H2O
-
Hafnia alvei DL-inositol 1,2,4,5,6-pentaphosphate + phosphate activity of EC 3.1.3.8, 10-14% of product ?
3.1.3.26 myo-inositol hexakisphosphate + H2O
-
Yersinia kristensenii DL-inositol 1,2,4,5,6-pentaphosphate + phosphate activity of EC 3.1.3.8, 10-14% of product ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.8 PhyA
-
Hafnia alvei
3.1.3.8 PhyA
-
Yersinia kristensenii
3.1.3.26 PhyA
-
Hafnia alvei
3.1.3.26 PhyA
-
Yersinia kristensenii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.8 55
-
-
Yersinia kristensenii
3.1.3.8 65
-
-
Hafnia alvei
3.1.3.26 55
-
-
Yersinia kristensenii
3.1.3.26 65
-
-
Hafnia alvei

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.8 57
-
melting temperature Yersinia kristensenii
3.1.3.8 70
-
melting temperature Hafnia alvei
3.1.3.26 57
-
melting temperature Yersinia kristensenii
3.1.3.26 70
-
melting temperature Hafnia alvei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.8 4 4.5
-
Hafnia alvei
3.1.3.8 4 4.5
-
Yersinia kristensenii
3.1.3.26 4 4.5
-
Hafnia alvei
3.1.3.26 4 4.5
-
Yersinia kristensenii