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Literature summary extracted from

  • Muehle, C.; Huttner, H.B.; Walter, S.; Reichel, M.; Canneva, F.; Lewczuk, P.; Gulbins, E.; Kornhuber, J.
    Characterization of acid sphingomyelinase activity in human cerebrospinal fluid (2013), PLoS ONE, 8, e62912.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.1.4.12 diagnostics the extracellular enzyme from cerebrospinal fluid might hava a value as a potential biomarker for disease type, severity, progress or therapeutic success Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.12 Zn2+ required for activity, best at 0.5-1 mM, inhibitory above 10 mM Homo sapiens
3.1.4.12 Zn2+ required for activity, best at 0.5-1 mM, inhibitory above 10 mM Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.12 additional information
-
 additional information Michaelis-Menten kinetics Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.12 extracellular the enzyme is secreted Mus musculus
-
-
3.1.4.12 extracellular the enzyme is secreted Homo sapiens
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.12 Zn2+ required for activity, best at 0.5-1 mM, inhibitory above 10 mM Mus musculus
3.1.4.12 Zn2+ required for activity, best at 0.5-1 mM, inhibitory above 10 mM Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.12 a sphingomyelin + H2O Mus musculus
-
 a ceramide + phosphocholine a ceramide is an N-acylsphingosine ?
3.1.4.12 a sphingomyelin + H2O Homo sapiens
-
 a ceramide + phosphocholine a ceramide is an N-acylsphingosine ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.12 Homo sapiens
-
 gene SMPD1
-
3.1.4.12 Mus musculus
-
 gene SMPD1
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.12 cerebrospinal fluid cerebrospinal fluid contains a detectable level of secretory enzyme, which is unrelated to serum enzyme activity, no influence of sex or age on enzyme activity in cerebrospinal fluid Mus musculus
-
3.1.4.12 cerebrospinal fluid cerebrospinal fluid contains a detectable level of secretory enzyme, which is unrelated to serum enzyme activity, no influence of sex or age on enzyme activity in cerebrospinal fluid Homo sapiens
-

Storage Stability

EC Number Storage Stability Organism
3.1.4.12 22°C, storage of very small volume cerebrospinal fluid aliquots, below 0.02 ml, at room temperature or above lead to the rapid loss of enzyme activity Homo sapiens
3.1.4.12 the enzyme keeps over 80% activity after 8 rounds of freezing and thawing Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.12 a sphingomyelin + H2O
-
 Mus musculus a ceramide + phosphocholine a ceramide is an N-acylsphingosine ?
3.1.4.12 a sphingomyelin + H2O
-
 Homo sapiens a ceramide + phosphocholine a ceramide is an N-acylsphingosine ?
3.1.4.12 a sphingomyelin + H2O using the fluorescent substrate BODIPY-FL-C12-SM, i.e. N-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-dodecanoyl)sphingosyl phosphocholine, D-7711 Homo sapiens a ceramide + phosphocholine a ceramide is an N-acylsphingosine ?
3.1.4.12 a sphingomyelin + H2O using the fluorescent substrate BODIPY-FL-C12-SM, N-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-dodecanoyl)sphingosyl phosphocholine, or D-7711 Mus musculus a ceramide + phosphocholine a ceramide is an N-acylsphingosine ?
3.1.4.12 additional information establishment of a sensitive quantitative assay method using the fluorescent substrate BODIPY-FL-C12-SM, i.e. N-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-dodecanoyl)sphingosyl phosphocholine, D-7711 Homo sapiens ?
-
 ?
3.1.4.12 additional information establishment of a sensitive quantitative assay method using the fluorescent substrate BODIPY-FL-C12-SM, N-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-dodecanoyl)sphingosyl phosphocholine, or D-7711 Mus musculus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.4.12 ASM
-
 Mus musculus
3.1.4.12 ASM
-
 Homo sapiens
3.1.4.12 sphingomyelinase
-
 Mus musculus
3.1.4.12 sphingomyelinase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.4.12 37
-
-
 Mus musculus
3.1.4.12 37
-
-
 Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.4.12 22
-
 storage of very small volume cerebrospinal fluid aliquots, below 0.02 ml, at room temperature or above lead to the rapid loss of enzyme activity Homo sapiens
3.1.4.12 40
-
 enzyme in cerebrospinal fluid, 2 h, stable under acidic conditions Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.12 5
-
 assay at Mus musculus
3.1.4.12 5
-
 assay at Homo sapiens

General Information

EC Number General Information Comment Organism
3.1.4.12 metabolism key enzyme in sphingolipid metabolism Mus musculus
3.1.4.12 metabolism key enzyme in sphingolipid metabolism Homo sapiens
3.1.4.12 physiological function as a key enzyme in sphingolipid metabolism, acid sphingomyelinase is involved in the regulation of cell fate and signaling via hydrolysis of sphingomyelin to form ceramide. Increased activity of the lysosomal form is associated with various pathological conditions Mus musculus
3.1.4.12 physiological function as a key enzyme in sphingolipid metabolism, acid sphingomyelinase is involved in the regulation of cell fate and signaling via hydrolysis of sphingomyelin to form ceramide. Increased activity of the lysosomal form is associated with various pathological conditions, the extracellular enzyme from cerebrospinal fluid migh also play a role in human disease Homo sapiens