EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.31.1 | transient replication of HPV-18 mutant ori plasmids with gene-delivered chimeric mutant AZP-SNase, overexpression of recombinant mutant chimeric enzyme, hybrid nuclease AZP-SNase, in Escherichia coli | Staphylococcus aureus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.1.31.1 | additional information | the enzyme is fused in a chimeric protein to artificial zinc-finger protein, which inhibits virus DNA replication in planta and in 293H cells by blocking binding of a viral replication protein to its replication origin. The resulting hybrid nuclease AZP-SNase cleaves its target DNA plasmid efficiently and sequence-specifically in vitro, and expressed in cells, it inhibits human papillomavirus HPV-18 DNA replication cleaving an HPV-18 ori plasmid around its binding site, overview | Staphylococcus aureus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.31.1 | Staphylococcus aureus | - |
- |
- |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.31.1 | SNase | - |
Staphylococcus aureus |
3.1.31.1 | staphylococcal nuclease | - |
Staphylococcus aureus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.31.1 | 37 | - |
assay at | Staphylococcus aureus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.31.1 | 7.5 | - |
assay at | Staphylococcus aureus |