EC Number | Cloned (Comment) | Organism |
---|---|---|
4.4.1.15 | epression of His6-tagged enzyme in Escherichia coli | Salmonella enterica subsp. enterica serovar Typhimurium |
4.5.1.2 | epression of His6-tagged enzyme in Escherichia coli | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
4.4.1.15 | purified enzyme in complex with D-Cys, beta-chloro-D-alanine, 1-amino-1-carboxy cyclopropane, D-Ser, L-Ser, D-cycloserine, and L-cycloserine, X-ray diffraction structure determination and analysis at 1.7-2.6 A resolution, modeling | Salmonella enterica subsp. enterica serovar Typhimurium |
4.5.1.2 | purified enzyme in complex with D-Cys, beta-chloro-D-alanine, 1-amino-1-carboxy cyclopropane, D-Ser, L-Ser, D-cycloserine, and L-cycloserine, X-ray diffraction structure determination and analysis at 1.7-2.6 A resolution, modeling | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.4.1.15 | H80Q | site-directed mutagenesis, the mutant shows altered substrate specificity and activity compared to the wild-type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
4.4.1.15 | Q77H | site-directed mutagenesis, the mutant is inactive with respect to D-Cys, but retains significant 30% activity with beta-chloro-D-alanine | Salmonella enterica subsp. enterica serovar Typhimurium |
4.4.1.15 | S78A | site-directed mutagenesis, the mutant is inactive with respect to D-Cys, but retains significant 20% activity with beta-chloro-D-alanine | Salmonella enterica subsp. enterica serovar Typhimurium |
4.4.1.15 | T288E | site-directed mutagenesis, the mutant shows altered substrate specificity and activity compared to the wild-type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
4.4.1.15 | T315L/T288E | site-directed mutagenesis, the mutant shows altered substrate specificity and activity compared to the wild-type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
4.4.1.15 | Y261F | site-directed mutagenesis, the mutant shows altered substrate specificity and activity compared to the wild-type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
4.4.1.15 | Y287F | site-directed mutagenesis, inactive mutant with all substrates tested | Salmonella enterica subsp. enterica serovar Typhimurium |
4.5.1.2 | H80Q | site-directed mutagenesis, the mutant shows altered substrate specificity and activity compared to the wild-type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
4.5.1.2 | Q77H | site-directed mutagenesis, the mutant is inactive with respect to D-Cys, but retains significant 30% activity with beta-chloro-D-alanine | Salmonella enterica subsp. enterica serovar Typhimurium |
4.5.1.2 | S78A | site-directed mutagenesis, the mutant is inactive with respect to D-Cys, but retains significant 20% activity with beta-chloro-D-alanine | Salmonella enterica subsp. enterica serovar Typhimurium |
4.5.1.2 | T288E | site-directed mutagenesis, the mutant shows altered substrate specificity and activity compared to the wild-type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
4.5.1.2 | T315L/T288E | site-directed mutagenesis, the mutant shows altered substrate specificity and activity compared to the wild-type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
4.5.1.2 | Y261F | site-directed mutagenesis, the mutant shows altered substrate specificity and activity compared to the wild-type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
4.5.1.2 | Y287F | site-directed mutagenesis, inactive mutant with all substrates tested | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.4.1.15 | 0.34 | - |
D-cysteine | recombinant His6-tagged enzyme, pH and temperature not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium | |
4.5.1.2 | 0.84 | - |
beta-Chloro-D-alanine | recombinant His6-tagged enzyme, pH and temperature not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.4.1.15 | D-cysteine + H2O | Salmonella enterica subsp. enterica serovar Typhimurium | - |
sulfide + NH3 + pyruvate | - |
? | |
4.5.1.2 | beta-chloro-D-alanine + H2O | Salmonella enterica subsp. enterica serovar Typhimurium | - |
pyruvate + chloride + NH3 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.4.1.15 | Salmonella enterica subsp. enterica serovar Typhimurium | Q8ZNT7 | - |
- |
4.5.1.2 | Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.4.1.15 | recombinant His6-tagged enzyme from Escherichia coli by nickel affinity chromatography | Salmonella enterica subsp. enterica serovar Typhimurium |
4.5.1.2 | recombinant His6-tagged enzyme from Escherichia coli by nickel affinity chromatography | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.4.1.15 | D-cysteine + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | sulfide + NH3 + pyruvate | - |
? | |
4.4.1.15 | D-cysteine + H2O | Ser78 and Gln77 are key determinants of enzyme specificity and the phenolate of Tyr287 is responsible for Calpha proton abstraction from D-Cys | Salmonella enterica subsp. enterica serovar Typhimurium | sulfide + NH3 + pyruvate | - |
? | |
4.4.1.15 | additional information | the enzyme is also active with beta-chloro-D-alanine which is converted to pyruvate, chloride, and NH3, EC 4.5.1.2. D-Ser is a poor substrate while the enzyme is inactive with respect to L-Ser and 1-amino-1-carboxy cyclopropane, substrate specificity and ligand binding structures, detailed overview. Ser78 and Gln77 are key determinants of enzyme specificity and the phenolate of Tyr287 is responsible for Calpha proton abstraction from D-Cys | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
4.5.1.2 | beta-chloro-D-alanine + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | pyruvate + chloride + NH3 | - |
? | |
4.5.1.2 | additional information | the enzyme mainly catalyzes the degradation of D-cysteine to sulfide, NH3, and pyruvate, EC 4.4.1.15. D-Ser is a poor substrate while the enzyme is inactive with respect to L-Ser and 1-amino-1-carboxy cyclopropane, substrate specificity and ligand binding structures, detailed overview. Ser78 and Gln77 are key determinants of enzyme specificity | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.4.1.15 | More | the polypeptide fold of the enzyme consists of a small domain (residues 48161) and a large domain (residues 1-47 and 162-328) | Salmonella enterica subsp. enterica serovar Typhimurium |
4.5.1.2 | More | the polypeptide fold of the enzyme consists of a small domain (residues 48-161) and a large domain (residues 1-47 and 162-328) | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.4.1.15 | DCyD | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
4.5.1.2 | DCyD | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
4.4.1.15 | pyridoxal 5'-phosphate | a fold type II pyridoxal 5'-phosphate-dependent enzyme | Salmonella enterica subsp. enterica serovar Typhimurium | |
4.5.1.2 | pyridoxal 5'-phosphate | a fold type II pyridoxal 5'-phosphate-dependent enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | General Information | Comment | Organism |
---|---|---|---|
4.4.1.15 | evolution | the enzyme structurally belongs to the fold type II pyridoxal 5'-phosphate-dependent enzyme family | Salmonella enterica subsp. enterica serovar Typhimurium |
4.4.1.15 | additional information | active site structure analysis and comparisons, residue Tr287 is important for catalysis, while His80 and Tyr261 may not be directly involved in the degradation of D-Cys, overview | Salmonella enterica subsp. enterica serovar Typhimurium |
4.5.1.2 | evolution | the enzyme structurally belongs to the fold type II pyridoxal 5'-phosphate-dependent enzyme family | Salmonella enterica subsp. enterica serovar Typhimurium |
4.5.1.2 | additional information | active site structure analysis and comparisons, residue Tr287 is important for catalysis, while His80 and Tyr261 may not be directly involved in the degradation of beta-chloro-D-alanine, overview | Salmonella enterica subsp. enterica serovar Typhimurium |