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Literature summary extracted from

  • Guan, X.; Lin, P.; Knoll, E.; Chakrabarti, R.
    Mechanism of inhibition of the human sirtuin enzyme SIRT3 by nicotinamide: computational and experimental studies (2014), PLoS One, 15, e107729.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.286 Isonicotinamide weak competitive inhibitor of hSIRT3 with respect to NAD+ in vitro; weak inhibitor Homo sapiens
2.3.1.286 nicotinamide mixed noncompetitive inhibitor. Inhibition of SIRT3 involves apparent competition between the inhibitor and the enzyme cofactor NAD+; predominantly noncompetitive inhibitor Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.286 Homo sapiens Q96EB6
-
-
2.3.1.286 Homo sapiens Q9NTG7
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.286 NAD+ + TRSG(N6-acetyl)KVMRRLLR acetylated substrate peptide based on the sequence of acetyl-coenzyme A synthetase 2 Homo sapiens nicotinamide + TRSGKVMRRLLR + 2'-O-acetyl-ADP-ribose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.286 SIRT1
-
 Homo sapiens
2.3.1.286 SIRT3
-
 Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.3.1.286 0.0367
-
 pH 8, 37°C Homo sapiens nicotinamide
2.3.1.286 0.0681
-
 pH 8, 37°C Homo sapiens nicotinamide
2.3.1.286 12200
-
 pH 8, 37°C, weak inhibitor Homo sapiens Isonicotinamide
2.3.1.286 13800
-
 pH 8, 37°C, weak competitive inhibitor of hSIRT3 with respect to NAD+ in vitro Homo sapiens Isonicotinamide