Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Caparros-Martin, J.A.; McCarthy-Suarez, I.; Culianez-Macia, F.A.
    The kinetic analysis of the substrate specificity of motif 5 in a HAD hydrolase-type phosphosugar phosphatase of Arabidopsis thaliana (2014), Planta, 240, 479-487.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.23 expressed in Escherichia coli TB1 cells Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.23 A69M the mutation slightly affects the substrate specificity Arabidopsis thaliana
3.1.3.23 H72K the mutant is inactive in phosphomonoester dephosphorylation Arabidopsis thaliana
3.1.3.23 I68M the mutation slightly affects the substrate specificity Arabidopsis thaliana
3.1.3.23 I68M/A69M/K71R/H72K the mutant is inactive in phosphomonoester dephosphorylation Arabidopsis thaliana
3.1.3.23 K71R the mutation slightly affects the substrate specificity Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.23 3.6
-
D-ribose 5-phosphate wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 3.8
-
D-ribose 5-phosphate mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 4.1
-
D-ribose 5-phosphate mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 4.6
-
2-deoxy-D-glucose 6-phosphate wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 4.9
-
D-mannose 6-phosphate wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 5
-
D-mannose 6-phosphate mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 5
-
2-deoxy-D-glucose 6-phosphate mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 5
-
2-deoxy-D-glucose 6-phosphate mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 5.5
-
D-mannose 6-phosphate mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 5.9
-
D-ribose 5-phosphate mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 5.9
-
2-deoxy-D-glucose 6-phosphate mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 6.7
-
D-mannose 6-phosphate mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 6.7
-
D-glucose 6-phosphate mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 7.1
-
DL-glycerol 3-phosphate mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 7.1
-
D-glucose 6-phosphate wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 7.3
-
DL-glycerol 3-phosphate wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 7.7
-
D-glucose 6-phosphate mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 7.7
-
D-fructose 6-phosphate wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 8
-
D-glucose 6-phosphate mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 8
-
D-fructose 6-phosphate mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 8
-
D-fructose 6-phosphate mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 8
-
DL-glycerol 3-phosphate mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 8.3
-
DL-glycerol 3-phosphate mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 9.1
-
D-fructose 6-phosphate mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.23 Mg2+ required for activity Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.23 42700
-
x * 42700, maltose-binding protein-bound enzyme, SDS-PAGE Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.21 DL-glycerol 3-phosphate + H2O Arabidopsis thaliana
-
glycerol + phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.21 Arabidopsis thaliana
-
-
-
3.1.3.23 Arabidopsis thaliana Q9ZVJ5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.23 amylose resin column chromatography Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.21 DL-glycerol 3-phosphate + H2O
-
Arabidopsis thaliana glycerol + phosphate
-
?
3.1.3.23 2-Deoxy-D-glucose 6-phosphate + H2O preferred substrate Arabidopsis thaliana 2-Deoxy-D-glucose + phosphate
-
?
3.1.3.23 2-deoxy-D-ribose 5-phosphate + H2O
-
Arabidopsis thaliana 2-deoxy-D-ribose + phosphate
-
?
3.1.3.23 D-fructose 1-phosphate + H2O
-
Arabidopsis thaliana D-fructose + phosphate
-
?
3.1.3.23 D-fructose 6-phosphate + H2O
-
Arabidopsis thaliana D-fructose + phosphate
-
?
3.1.3.23 D-glucose 6-phosphate + H2O
-
Arabidopsis thaliana D-glucose + phosphate
-
?
3.1.3.23 D-mannose 6-phosphate + H2O preferred substrate Arabidopsis thaliana D-mannose + phosphate
-
?
3.1.3.23 D-ribose 5-phosphate + H2O preferred substrate Arabidopsis thaliana D-ribose + phosphate
-
?
3.1.3.23 DL-glycerol 3-phosphate + H2O
-
Arabidopsis thaliana glycerol + phosphate
-
?

Subunits

EC Number Subunits Comment Organism
3.1.3.23 ? x * 42700, maltose-binding protein-bound enzyme, SDS-PAGE Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
3.1.3.21 DL-glycerol-3-phosphatase
-
Arabidopsis thaliana
3.1.3.21 GPP
-
Arabidopsis thaliana
3.1.3.23 phosphosugar phosphatase
-
Arabidopsis thaliana
3.1.3.23 Sgpp
-
Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.23 3.6
-
D-fructose 6-phosphate mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 6.6
-
DL-glycerol 3-phosphate mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 7.1
-
D-glucose 6-phosphate mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 19
-
D-fructose 6-phosphate mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 19
-
D-fructose 6-phosphate mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 19
-
D-fructose 6-phosphate wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 22
-
DL-glycerol 3-phosphate mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 23
-
DL-glycerol 3-phosphate wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 24
-
DL-glycerol 3-phosphate mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 24
-
D-glucose 6-phosphate mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 24
-
D-glucose 6-phosphate wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 26
-
D-mannose 6-phosphate mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 26
-
D-glucose 6-phosphate mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 31
-
2-deoxy-D-glucose 6-phosphate mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 32
-
D-mannose 6-phosphate mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 32
-
D-mannose 6-phosphate wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 33
-
D-ribose 5-phosphate mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 33
-
D-mannose 6-phosphate mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 33
-
2-deoxy-D-glucose 6-phosphate mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 33
-
2-deoxy-D-glucose 6-phosphate wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 34
-
2-deoxy-D-glucose 6-phosphate mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 39
-
D-ribose 5-phosphate mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 39
-
D-ribose 5-phosphate mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 39
-
D-ribose 5-phosphate wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.23 7
-
-
Arabidopsis thaliana

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.3.23 0.4
-
D-fructose 6-phosphate mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 0.8
-
DL-glycerol 3-phosphate mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 0.9
-
D-glucose 6-phosphate mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 2.4
-
D-fructose 6-phosphate mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 2.4
-
D-fructose 6-phosphate mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 2.5
-
D-fructose 6-phosphate wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 2.8
-
DL-glycerol 3-phosphate mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 3.1
-
DL-glycerol 3-phosphate wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 3.2
-
D-glucose 6-phosphate mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 3.4
-
DL-glycerol 3-phosphate mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 3.4
-
D-glucose 6-phosphate wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 3.8
-
D-mannose 6-phosphate mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 3.8
-
D-glucose 6-phosphate mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 5.2
-
2-deoxy-D-glucose 6-phosphate mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 5.6
-
D-ribose 5-phosphate mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 5.8
-
D-mannose 6-phosphate mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 6.6
-
D-mannose 6-phosphate mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 6.6
-
2-deoxy-D-glucose 6-phosphate mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 6.7
-
D-mannose 6-phosphate wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 6.9
-
2-deoxy-D-glucose 6-phosphate mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 7.2
-
2-deoxy-D-glucose 6-phosphate wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 9.4
-
D-ribose 5-phosphate mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 10.2
-
D-ribose 5-phosphate mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1.3.23 10.7
-
D-ribose 5-phosphate wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana