EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.3.23 | expressed in Escherichia coli TB1 cells | Arabidopsis thaliana |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.1.3.23 | A69M | the mutation slightly affects the substrate specificity | Arabidopsis thaliana |
3.1.3.23 | H72K | the mutant is inactive in phosphomonoester dephosphorylation | Arabidopsis thaliana |
3.1.3.23 | I68M | the mutation slightly affects the substrate specificity | Arabidopsis thaliana |
3.1.3.23 | I68M/A69M/K71R/H72K | the mutant is inactive in phosphomonoester dephosphorylation | Arabidopsis thaliana |
3.1.3.23 | K71R | the mutation slightly affects the substrate specificity | Arabidopsis thaliana |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.23 | 3.6 | - |
D-ribose 5-phosphate | wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 3.8 | - |
D-ribose 5-phosphate | mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 4.1 | - |
D-ribose 5-phosphate | mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 4.6 | - |
2-deoxy-D-glucose 6-phosphate | wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 4.9 | - |
D-mannose 6-phosphate | wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 5 | - |
D-mannose 6-phosphate | mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 5 | - |
2-deoxy-D-glucose 6-phosphate | mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 5 | - |
2-deoxy-D-glucose 6-phosphate | mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 5.5 | - |
D-mannose 6-phosphate | mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 5.9 | - |
D-ribose 5-phosphate | mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 5.9 | - |
2-deoxy-D-glucose 6-phosphate | mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 6.7 | - |
D-mannose 6-phosphate | mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 6.7 | - |
D-glucose 6-phosphate | mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 7.1 | - |
DL-glycerol 3-phosphate | mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 7.1 | - |
D-glucose 6-phosphate | wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 7.3 | - |
DL-glycerol 3-phosphate | wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 7.7 | - |
D-glucose 6-phosphate | mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 7.7 | - |
D-fructose 6-phosphate | wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 8 | - |
D-glucose 6-phosphate | mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 8 | - |
D-fructose 6-phosphate | mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 8 | - |
D-fructose 6-phosphate | mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 8 | - |
DL-glycerol 3-phosphate | mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 8.3 | - |
DL-glycerol 3-phosphate | mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 9.1 | - |
D-fructose 6-phosphate | mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.23 | Mg2+ | required for activity | Arabidopsis thaliana |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.3.23 | 42700 | - |
x * 42700, maltose-binding protein-bound enzyme, SDS-PAGE | Arabidopsis thaliana |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.21 | DL-glycerol 3-phosphate + H2O | Arabidopsis thaliana | - |
glycerol + phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.3.21 | Arabidopsis thaliana | - |
- |
- |
3.1.3.23 | Arabidopsis thaliana | Q9ZVJ5 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.3.23 | amylose resin column chromatography | Arabidopsis thaliana |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.21 | DL-glycerol 3-phosphate + H2O | - |
Arabidopsis thaliana | glycerol + phosphate | - |
? | |
3.1.3.23 | 2-Deoxy-D-glucose 6-phosphate + H2O | preferred substrate | Arabidopsis thaliana | 2-Deoxy-D-glucose + phosphate | - |
? | |
3.1.3.23 | 2-deoxy-D-ribose 5-phosphate + H2O | - |
Arabidopsis thaliana | 2-deoxy-D-ribose + phosphate | - |
? | |
3.1.3.23 | D-fructose 1-phosphate + H2O | - |
Arabidopsis thaliana | D-fructose + phosphate | - |
? | |
3.1.3.23 | D-fructose 6-phosphate + H2O | - |
Arabidopsis thaliana | D-fructose + phosphate | - |
? | |
3.1.3.23 | D-glucose 6-phosphate + H2O | - |
Arabidopsis thaliana | D-glucose + phosphate | - |
? | |
3.1.3.23 | D-mannose 6-phosphate + H2O | preferred substrate | Arabidopsis thaliana | D-mannose + phosphate | - |
? | |
3.1.3.23 | D-ribose 5-phosphate + H2O | preferred substrate | Arabidopsis thaliana | D-ribose + phosphate | - |
? | |
3.1.3.23 | DL-glycerol 3-phosphate + H2O | - |
Arabidopsis thaliana | glycerol + phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.3.23 | ? | x * 42700, maltose-binding protein-bound enzyme, SDS-PAGE | Arabidopsis thaliana |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.3.21 | DL-glycerol-3-phosphatase | - |
Arabidopsis thaliana |
3.1.3.21 | GPP | - |
Arabidopsis thaliana |
3.1.3.23 | phosphosugar phosphatase | - |
Arabidopsis thaliana |
3.1.3.23 | Sgpp | - |
Arabidopsis thaliana |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.23 | 3.6 | - |
D-fructose 6-phosphate | mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 6.6 | - |
DL-glycerol 3-phosphate | mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 7.1 | - |
D-glucose 6-phosphate | mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 19 | - |
D-fructose 6-phosphate | mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 19 | - |
D-fructose 6-phosphate | mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 19 | - |
D-fructose 6-phosphate | wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 22 | - |
DL-glycerol 3-phosphate | mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 23 | - |
DL-glycerol 3-phosphate | wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 24 | - |
DL-glycerol 3-phosphate | mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 24 | - |
D-glucose 6-phosphate | mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 24 | - |
D-glucose 6-phosphate | wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 26 | - |
D-mannose 6-phosphate | mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 26 | - |
D-glucose 6-phosphate | mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 31 | - |
2-deoxy-D-glucose 6-phosphate | mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 32 | - |
D-mannose 6-phosphate | mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 32 | - |
D-mannose 6-phosphate | wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 33 | - |
D-ribose 5-phosphate | mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 33 | - |
D-mannose 6-phosphate | mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 33 | - |
2-deoxy-D-glucose 6-phosphate | mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 33 | - |
2-deoxy-D-glucose 6-phosphate | wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 34 | - |
2-deoxy-D-glucose 6-phosphate | mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 39 | - |
D-ribose 5-phosphate | mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 39 | - |
D-ribose 5-phosphate | mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 39 | - |
D-ribose 5-phosphate | wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.3.23 | 7 | - |
- |
Arabidopsis thaliana |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.23 | 0.4 | - |
D-fructose 6-phosphate | mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 0.8 | - |
DL-glycerol 3-phosphate | mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 0.9 | - |
D-glucose 6-phosphate | mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 2.4 | - |
D-fructose 6-phosphate | mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 2.4 | - |
D-fructose 6-phosphate | mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 2.5 | - |
D-fructose 6-phosphate | wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 2.8 | - |
DL-glycerol 3-phosphate | mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 3.1 | - |
DL-glycerol 3-phosphate | wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 3.2 | - |
D-glucose 6-phosphate | mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 3.4 | - |
DL-glycerol 3-phosphate | mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 3.4 | - |
D-glucose 6-phosphate | wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 3.8 | - |
D-mannose 6-phosphate | mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 3.8 | - |
D-glucose 6-phosphate | mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 5.2 | - |
2-deoxy-D-glucose 6-phosphate | mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 5.6 | - |
D-ribose 5-phosphate | mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 5.8 | - |
D-mannose 6-phosphate | mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 6.6 | - |
D-mannose 6-phosphate | mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 6.6 | - |
2-deoxy-D-glucose 6-phosphate | mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 6.7 | - |
D-mannose 6-phosphate | wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 6.9 | - |
2-deoxy-D-glucose 6-phosphate | mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 7.2 | - |
2-deoxy-D-glucose 6-phosphate | wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 9.4 | - |
D-ribose 5-phosphate | mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 10.2 | - |
D-ribose 5-phosphate | mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana | |
3.1.3.23 | 10.7 | - |
D-ribose 5-phosphate | wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C | Arabidopsis thaliana |