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Literature summary extracted from

  • Li, B.; Zhao, Y.; Liang, L.; Ren, H.; Xing, Y.; Chen, L.; Sun, M.; Wang, Y.; Han, Y.; Jia, H.; Huang, C.; Wu, Z.; Jia, W.
    Purification and characterization of ZmRIP1, a novel reductant-inhibited protein tyrosine phosphatase from maize (2012), Plant Physiol., 159, 671-681.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.48 expressed in Saccharomyces cerevisiae and Arabidopsis thaliana Zea mays

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.48 dithiothreitol complete inhibition at 1.8 mM Zea mays
3.1.3.48 additional information shows no sensitivity to H2O2 Zea mays
3.1.3.48 PAO complete inhibition at 0.0.5 mM Zea mays

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.48 chloroplast
-
 Zea mays 9507
-
3.1.3.48 nucleus he enzyme migrates from chloroplasts to nuclei in response to an oxidative stimulus Zea mays 5634
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.48 additional information not influenced by Ca2+, Mg2+, and Mn2+ Zea mays

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.48 42000
-
 x * 42000, SDS-PAGE Zea mays

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.48 Zea mays B4FJN0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.48 DEAE column column chromatography, SP Sepharose column chromatography, Sephacryl S-200 gel filtration, and Sephacryl S-300 gel filtration Zea mays

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.48 coleoptile
-
 Zea mays
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.48 2110
-
 crude extract, at pH and °C Zea mays
3.1.3.48 580000
-
 after 1611fold purification, at pH and °C Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.48 4-nitrophenyl phosphate + H2O
-
 Zea mays 4-nitrophenol + phosphate
-
 ?
3.1.3.48 [EGF receptor]-tyrosine1018 phosphate + H2O highest activity Zea mays [EGF receptor]-tyrosine1018 + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.48 ? x * 42000, SDS-PAGE Zea mays

Synonyms

EC Number Synonyms Comment Organism
3.1.3.48 protein tyrosine phosphatase
-
 Zea mays
3.1.3.48 PTPase
-
 Zea mays
3.1.3.48 reductant-inhibited PTPase1
-
 Zea mays
3.1.3.48 RIP1
-
 Zea mays

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.48 5
-
-
 Zea mays

General Information

EC Number General Information Comment Organism
3.1.3.48 physiological function the enzyme functions in redox signaling Zea mays