EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.3.23 | expressed in Escherichia coli BL21(DE3)pLysS cells | Plasmodium falciparum |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.1.3.23 | hanging drop vapor diffusion method, using 0.1M HEPES (pH 7.5) and 20-25% (w/v) PEG 8000 | Plasmodium falciparum |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.1.3.23 | N70S | the mutant shows wild type activity | Plasmodium falciparum |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.1.3.23 | cytoplasm | - |
Plasmodium falciparum | 5737 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.23 | Mg2+ | contains Mg2+ in the active site | Plasmodium falciparum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.3.23 | 33000 | - |
x * 33000, SDS-PAGE | Plasmodium falciparum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.23 | D-fructose 1-phosphate + H2O | Plasmodium falciparum | best substrate | D-fructose + phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.3.23 | Plasmodium falciparum | Q8IJ74 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.3.23 | nickel agarose bead chromatography and Superdex 200 gel filtration | Plasmodium falciparum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.23 | 4-nitrophenyl phosphate + H2O | - |
Plasmodium falciparum | 4-nitrophenol + phosphate | - |
? | |
3.1.3.23 | D-erythrose 4-phosphate + H2O | - |
Plasmodium falciparum | D-erythrose + phosphate | - |
? | |
3.1.3.23 | D-fructose 1-phosphate + H2O | best substrate | Plasmodium falciparum | D-fructose + phosphate | - |
? | |
3.1.3.23 | D-fructose 6-phosphate + H2O | - |
Plasmodium falciparum | D-fructose + phosphate | - |
? | |
3.1.3.23 | D-glucose 1-phosphate + H2O | - |
Plasmodium falciparum | D-glucose + phosphate | - |
? | |
3.1.3.23 | D-glucose 2-phosphate + H2O | second best substrate | Plasmodium falciparum | D-glucose + phosphate | - |
? | |
3.1.3.23 | D-glucose 6-phosphate + H2O | - |
Plasmodium falciparum | D-glucose + phosphate | - |
? | |
3.1.3.23 | D-mannose 6-phosphate + H2O | - |
Plasmodium falciparum | D-mannose + phosphate | - |
? | |
3.1.3.23 | D-ribose 5-phosphate + H2O | - |
Plasmodium falciparum | D-ribose + phosphate | - |
? | |
3.1.3.23 | D-ribulose 5-phosphate + H2O | - |
Plasmodium falciparum | D-ribulose + phosphate | - |
? | |
3.1.3.23 | deoxyribose 5-phosphate + H2O | - |
Plasmodium falciparum | deoxyribose + phosphate | - |
? | |
3.1.3.23 | deoxyxylulose 5-phosphate + H2O | - |
Plasmodium falciparum | deoxyxylulose + phosphate | - |
? | |
3.1.3.23 | dihydroxyacetone phosphate + H2O | - |
Plasmodium falciparum | dihydroxyacetone + phosphate | - |
? | |
3.1.3.23 | methylerythritol phosphate + H2O | - |
Plasmodium falciparum | methylerythritol + phosphate | - |
? | |
3.1.3.23 | additional information | low activity towards D-fructose 1,6-bisphosphate, D-galactose 1-phosphate, glycerol 2-phosphate, glucosamine 1-phosphate, D-mannose 1-phosphate, phosphoenolpyruvate, sedoheptulose 7-phosphate, sorbitol 6-phosphate, trehalose 6-phosphate, 2-phosphoglyceric acid, and 3-phosphoglyceric acid | Plasmodium falciparum | ? | - |
? | |
3.1.3.23 | additional information | the enzyme is most active against monophosphorylated three to six-carbon monosaccharides | Plasmodium falciparum | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.3.23 | ? | x * 33000, SDS-PAGE | Plasmodium falciparum |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.3.23 | HAD1 | - |
Plasmodium falciparum |
3.1.3.23 | haloacid dehalogenase-like sugar phosphatase | - |
Plasmodium falciparum |
3.1.3.23 | PF3D7 1033400 | locus name | Plasmodium falciparum |
3.1.3.23 | sugar phosphatase | - |
Plasmodium falciparum |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.1.3.23 | malfunction | loss of HAD1 is required for fosmidomycin resistance. Parasites lacking HAD1 have increased methylerythritol phosphate pathway metabolites, particularly the deoxyxylulose 5-phosphate reductoisomerase substrate deoxyxylulose 5-phosphate | Plasmodium falciparum |
3.1.3.23 | metabolism | HAD1 controls substrate availability to the methylerythritol phosphate pathway | Plasmodium falciparum |