BRENDA - Enzyme Database

FadD3 is an acyl-CoA synthetase that initiates catabolism of cholesterol rings C and D in actinobacteria

Casabon, I.; Crowe, A.M.; Liu, J.; Eltis, L.D.; Mol. Microbiol. 87, 269-283 (2013)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
6.2.1.41
expressed in Escherichia coli Rosetta(DE3)pLysS cells
Mycobacterium tuberculosis
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
6.2.1.41
additional information
neither 1beta(2'-propanoate)-3aalpha-H-4a(3''-propanoate)-7alphabeta-methylhexahydro-5-indanone nor 3aalpha-H-4aalpha(3'-propanoic acid)-5alpha-hydroxy-7alphabeta-methylhexahydro-1-indanone-D-lactone significantly inhibit enzyme activity
Mycobacterium tuberculosis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.2.1.41
0.0098
-
3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoate
at pH 7.3 and 22C
Mycobacterium tuberculosis
6.2.1.41
0.11
-
CoA
at pH 7.3 and 22C
Mycobacterium tuberculosis
6.2.1.41
3
-
3aalpha-H-4a(3'-propanoate)-5alpha-hydroxy-7alphabeta-methylhexahydro-1-indanone
at pH 7.3 and 22C
Mycobacterium tuberculosis
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
6.2.1.41
ATP + 3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoate + CoA
Mycobacterium tuberculosis
-
AMP + diphosphate + 3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoyl-CoA
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
6.2.1.41
Mycobacterium tuberculosis
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
6.2.1.41
Ni-Sepharose 6 column chromatography
Mycobacterium tuberculosis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
6.2.1.41
ATP + 3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoate + CoA
-
730411
Mycobacterium tuberculosis
AMP + diphosphate + 3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoyl-CoA
-
-
-
?
6.2.1.41
ATP + 3aalpha-H-4a(3'-propanoate)-5alpha-hydroxy-7alphabeta-methylhexahydro-1-indanone
-
730411
Mycobacterium tuberculosis
?
-
-
-
?
6.2.1.41
additional information
no activity with 3aalpha-H-4a(3'-propanoate)-5alpha-hydroxy-7alphabeta-methylhexahydro-1-indanone-D-lactone, 3-oxo-23,24-bisnorchol-4-en-22-oate, cholate, and 3beta-hydroxy-5-cholesten-26-oate
730411
Mycobacterium tuberculosis
?
-
-
-
-
Synonyms
EC Number
Synonyms
Commentary
Organism
6.2.1.41
fadD3
-
Mycobacterium tuberculosis
6.2.1.41
HIP-CoA ligase
-
Mycobacterium tuberculosis
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
6.2.1.41
7.1
-
3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoate
at pH 7.3 and 22C
Mycobacterium tuberculosis
6.2.1.41
7.5
-
CoA
at pH 7.3 and 22C
Mycobacterium tuberculosis
6.2.1.41
13
-
3aalpha-H-4a(3'-propanoate)-5alpha-hydroxy-7alphabeta-methylhexahydro-1-indanone
at pH 7.3 and 22C
Mycobacterium tuberculosis
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
6.2.1.41
expressed in Escherichia coli Rosetta(DE3)pLysS cells
Mycobacterium tuberculosis
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
6.2.1.41
additional information
neither 1beta(2'-propanoate)-3aalpha-H-4a(3''-propanoate)-7alphabeta-methylhexahydro-5-indanone nor 3aalpha-H-4aalpha(3'-propanoic acid)-5alpha-hydroxy-7alphabeta-methylhexahydro-1-indanone-D-lactone significantly inhibit enzyme activity
Mycobacterium tuberculosis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.2.1.41
0.0098
-
3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoate
at pH 7.3 and 22C
Mycobacterium tuberculosis
6.2.1.41
0.11
-
CoA
at pH 7.3 and 22C
Mycobacterium tuberculosis
6.2.1.41
3
-
3aalpha-H-4a(3'-propanoate)-5alpha-hydroxy-7alphabeta-methylhexahydro-1-indanone
at pH 7.3 and 22C
Mycobacterium tuberculosis
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
6.2.1.41
ATP + 3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoate + CoA
Mycobacterium tuberculosis
-
AMP + diphosphate + 3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoyl-CoA
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
6.2.1.41
Ni-Sepharose 6 column chromatography
Mycobacterium tuberculosis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
6.2.1.41
ATP + 3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoate + CoA
-
730411
Mycobacterium tuberculosis
AMP + diphosphate + 3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoyl-CoA
-
-
-
?
6.2.1.41
ATP + 3aalpha-H-4a(3'-propanoate)-5alpha-hydroxy-7alphabeta-methylhexahydro-1-indanone
-
730411
Mycobacterium tuberculosis
?
-
-
-
?
6.2.1.41
additional information
no activity with 3aalpha-H-4a(3'-propanoate)-5alpha-hydroxy-7alphabeta-methylhexahydro-1-indanone-D-lactone, 3-oxo-23,24-bisnorchol-4-en-22-oate, cholate, and 3beta-hydroxy-5-cholesten-26-oate
730411
Mycobacterium tuberculosis
?
-
-
-
-
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
6.2.1.41
7.1
-
3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoate
at pH 7.3 and 22C
Mycobacterium tuberculosis
6.2.1.41
7.5
-
CoA
at pH 7.3 and 22C
Mycobacterium tuberculosis
6.2.1.41
13
-
3aalpha-H-4a(3'-propanoate)-5alpha-hydroxy-7alphabeta-methylhexahydro-1-indanone
at pH 7.3 and 22C
Mycobacterium tuberculosis
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
6.2.1.41
4.4
-
3aalpha-H-4a(3'-propanoate)-5alpha-hydroxy-7alphabeta-methylhexahydro-1-indanone
at pH 7.3 and 22C
Mycobacterium tuberculosis
6.2.1.41
66
-
CoA
at pH 7.3 and 22C
Mycobacterium tuberculosis
6.2.1.41
730
-
3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoate
at pH 7.3 and 22C
Mycobacterium tuberculosis
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
6.2.1.41
4.4
-
3aalpha-H-4a(3'-propanoate)-5alpha-hydroxy-7alphabeta-methylhexahydro-1-indanone
at pH 7.3 and 22C
Mycobacterium tuberculosis
6.2.1.41
66
-
CoA
at pH 7.3 and 22C
Mycobacterium tuberculosis
6.2.1.41
730
-
3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoate
at pH 7.3 and 22C
Mycobacterium tuberculosis