EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.8 | overexpression in Pichia pastoris | Streptomyces sp. |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.2.1.8 | additional information | recombinant protein expressed in Pichia pastoris without signal peptide exhibits better physicochemical properties than those of the native enzyme including higher optimal temperature of 60°C, and specific activity, but lower optimal pH 4.0 | Streptomyces sp. |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.2.1.8 | extracellular | - |
Streptomyces sp. | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.8 | 24000 | - |
x * 24000, SDS-PAGE | Streptomyces sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.8 | Streptomyces sp. | F7J663 | - |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.2.1.8 | glycoprotein | sequence contains six potential glycosylation sites | Streptomyces sp. |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.8 | - |
Streptomyces sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.8 | 13.3 | - |
native enzyme, pH 6.0, 37°C | Streptomyces sp. |
3.2.1.8 | 77.4 | - |
recombinant enzyme, pH 6.0, 37°C | Streptomyces sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.8 | birchwood xylan + H2O | - |
Streptomyces sp. | ? | - |
? | |
3.2.1.8 | oat spelt xylan + H2O | 52% of the activity with birchwood xylan | Streptomyces sp. | xylobiose + xylotriose + ? | major products | ? | |
3.2.1.8 | wheat arabinoxylan + H2O | 35% of the activity with birchwood xylan | Streptomyces sp. | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.8 | ? | x * 24000, SDS-PAGE | Streptomyces sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.8 | XynSW1 | - |
Streptomyces sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.8 | 40 | - |
- |
Streptomyces sp. |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.8 | 50 | - |
stable up to, for 1 h | Streptomyces sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.8 | 5 | - |
- |
Streptomyces sp. |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.8 | 2 | 11 | 4°C, 16 h, more than 80% of initial activity | Streptomyces sp. |