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Literature summary extracted from

  • Deesukon, W.; Nishimura, Y.; Sakamoto, T.; Sukhumsirichart, W.
    Purification, characterization of GH11 endo-beta-1,4-xylanase from thermotolerant Streptomyces sp. SWU10 and overexpression in Pichia pastoris KM71H (2013), Mol. Biotechnol., 54, 37-46.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.8 overexpression in Pichia pastoris Streptomyces sp.

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.8 additional information recombinant protein expressed in Pichia pastoris without signal peptide exhibits better physicochemical properties than those of the native enzyme including higher optimal temperature of 60°C, and specific activity, but lower optimal pH 4.0 Streptomyces sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.8 extracellular
-
Streptomyces sp.
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.8 24000
-
x * 24000, SDS-PAGE Streptomyces sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Streptomyces sp. F7J663
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.8 glycoprotein sequence contains six potential glycosylation sites Streptomyces sp.

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.8
-
Streptomyces sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.8 13.3
-
native enzyme, pH 6.0, 37°C Streptomyces sp.
3.2.1.8 77.4
-
recombinant enzyme, pH 6.0, 37°C Streptomyces sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 birchwood xylan + H2O
-
Streptomyces sp. ?
-
?
3.2.1.8 oat spelt xylan + H2O 52% of the activity with birchwood xylan Streptomyces sp. xylobiose + xylotriose + ? major products ?
3.2.1.8 wheat arabinoxylan + H2O 35% of the activity with birchwood xylan Streptomyces sp. ?
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.8 ? x * 24000, SDS-PAGE Streptomyces sp.

Synonyms

EC Number Synonyms Comment Organism
3.2.1.8 XynSW1
-
Streptomyces sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 40
-
-
Streptomyces sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.8 50
-
stable up to, for 1 h Streptomyces sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 5
-
-
Streptomyces sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.8 2 11 4°C, 16 h, more than 80% of initial activity Streptomyces sp.