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Literature summary extracted from

  • Kakimoto, M.; Ishikawa, T.; Miyagi, A.; Saito, K.; Miyazaki, M.; Asaeda, T.; Yamaguchi, M.; Uchimiya, H.; Kawai-Yamada, M.
    Culture temperature affects gene expression and metabolic pathways in the 2-methylisoborneol-producing cyanobacterium Pseudanabaena galeata (2014), J. Plant Physiol., 171, 292-300.
    View publication on PubMed

Application

EC Number Application Comment Organism
4.2.3.118 nutrition 2-methylisoborneol produced by cyanobacteria,, causes an unpleasant taste and odor in tap water. Bacterial cultures show higher accumulation of 2-methylisoborneol at 30°C than at 4°C or 20°C after 24 h of culture Pseudanabaena galeata

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.255 gene pgmtf, real-time-RT PCR enzyme expression analysis Pseudanabaena galeata

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.255 S-adenosyl-L-methionine + geranyl diphosphate Pseudanabaena galeata
-
 S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.255 Pseudanabaena galeata U6C870 gene pgmtf
-
4.2.3.118 Pseudanabaena galeata U6C722
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.255 S-adenosyl-L-methionine + geranyl diphosphate
-
 Pseudanabaena galeata S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.255 GPP methyltransferase
-
 Pseudanabaena galeata
2.1.1.255 GPPMT
-
 Pseudanabaena galeata

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.255 S-adenosyl-L-methionine
-
 Pseudanabaena galeata

Expression

EC Number Organism Comment Expression
2.1.1.255 Pseudanabaena galeata the expression level of the gene encoding GPPMT decreases at 4°C and increased at 30°C, compared with the level at 20°C down
2.1.1.255 Pseudanabaena galeata the expression level of the gene encoding GPPMT decreases at 4°C and increased at 30°C, compared with the level at 20°C up
4.2.3.118 Pseudanabaena galeata the expression level decreases at 4°C, compared with at 20°C down
4.2.3.118 Pseudanabaena galeata the expression level increases at 30°C, compared with at 20°C up

General Information

EC Number General Information Comment Organism
2.1.1.255 metabolism some filamentous cyanobacteria produce 2-methylborneol via a two-step biosynthetic pathway: methylation of geranyl diphosphate (GPP) by methyl transferase (GPPMT), followed by the cyclization of methyl-GPP by monoterpene cyclase (MIBS). Pseudanabaena galeata, the filamentous cyanobacterium known to be a major causal organism of 2-methylborneol production in Japanese lakes. Metabolite analysis shows dramatic changes in primary metabolite concentrations in cyanobacteria grown at different temperatures. The data indicate that changes in carbon flow in the TCA cycle affect 2-MIB biosynthesis at higher temperatures Pseudanabaena galeata