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Literature summary extracted from

  • Allan, J.; Fraser, R.M.; Owen-Hughes, T.; Keszenman-Pereyra, D.
    Micrococcal nuclease does not substantially bias nucleosome mapping (2012), J. Mol. Biol., 417, 152-164.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.1.31.1 analysis the enzyme is very widely used to map nucleosomes Staphylococcus aureus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.31.1 crystal structure analysis, PDB ID 2SNS Staphylococcus aureus

Organism

EC Number Organism UniProt Comment Textmining
3.1.31.1 Staphylococcus aureus P00644
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.31.1 additional information substrates are chicken and recombinant frog chromatins, as well as mixture of two plasmid DNAs, one harbouring a 10841-bp segment of sheep DNA containing the beta-lactoglobulin gene and the other harbouring a 13626-bp segment of Saccharomyces cerevisiae DNA incorporating a late-firing replication yeast replication origin, reconstituted with limiting amounts of core histones by salt gradient dialysis. Chromatins, prepared by reconstitution with either chicken or frog histones, are digested to mononucleosomes using micrococcal nuclease, identification of the locations and quantification of the strength of both the chicken or frog histone octamer binding sites on each DNA, the enzyme shows sequence specificity in its preferred cleavage sites with a preference to cut at sites centred on A/T-containing dinucleotides, and comparison to the activity of caspase-activated DNase, overview Staphylococcus aureus ?
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 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.31.1 MNase
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 Staphylococcus aureus