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Literature summary extracted from

  • Park, J.N.; Han, C.P.; Lee, D.J.; Cheon, S.A.; Kang, H.A.
    Functional characterization of extracellular chitinase encoded by the YlCTS1 gene in a dimorphic yeast Yarrowia lipolytica (2014), J. Microbiol., 52, 284-291.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.14 analysis isozyme YlCts1p can be used as a good model for studying O-glycosylation in the dimorphic yeast Yersinia lipolytica Yarrowia lipolytica

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.14 gene YlCTS1, DNA and amino acid sequence determination, analysis and comparison Yarrowia lipolytica

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.14 extracellular the enzyme is a secreted glycoprotein modified with intensive O-mannosylation Yarrowia lipolytica
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.14 chitin + H2O Yarrowia lipolytica
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Yarrowia lipolytica
-
 gene YlCTS1
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.14 glycoprotein the isozyme YlCts1p is modified only by O-mannosylation without N-glycosylation, deglycosylation by jack bean alpha-mannosidase digestion but not by PNGase F treatment Yarrowia lipolytica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 4-methylumbelliferyl beta-D-N,N',N''-triacetylchitotrioside + H2O
-
 Yarrowia lipolytica 4-methylumbelliferol + beta-D-N,N',N''-triacetylchitotriose
-
 ?
3.2.1.14 chitin + H2O
-
 Yarrowia lipolytica ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.14 endochitinase
-
 Yarrowia lipolytica
3.2.1.14 YlCts1p
-
 Yarrowia lipolytica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.14 28
-
 assay at Yarrowia lipolytica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 5
-
 assay at Yarrowia lipolytica

General Information

EC Number General Information Comment Organism
3.2.1.14 evolution the enzyme is a glycoside hydrolase family 18 chitinase Yarrowia lipolytica
3.2.1.14 malfunction isozyme YlCts1p deletion results in remarkably reduced extracellular endochitinase activity in the culture supernatant of Yersinia lipolytica and enhanced cell aggregation Yarrowia lipolytica
3.2.1.14 additional information isozyme YlCts1p contains an N-terminal secretion signal peptide, a long C-terminal Ser/Thr-rich domain, and a chitin-binding domain Yarrowia lipolytica
3.2.1.14 physiological function chitinase cleaves the beta-1,4-linkage between N-acetyl-D-glucosamine residues in the linear homopolymer chitin, which is an essential component of yeast and fungal cell walls Yarrowia lipolytica