Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Roach, C.R.; Hall, D.E.; Zerbe, P.; Bohlmann, J.
    Plasticity and evolution of (+)-3-carene synthase and (-)-sabinene synthase functions of a Sitka spruce monoterpene synthase gene family associated with weevil resistance (2014), J. Biol. Chem., 289, 23859-23869.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.107
-
 Picea sitchensis
4.2.3.109
-
 Picea sitchensis
4.2.3.113
-
 Picea sitchensis

Protein Variants

EC Number Protein Variants Comment Organism
4.2.3.107 G595A/L596F/F599L shift in product profile, mutant produces 1.8% (+)-car-3-ene, 23.3% (-)-sabinene, 55.2% terpinolene Picea sitchensis
4.2.3.107 G595A/L596F/F599L shift in product profile, mutant produces 4.7% (+)-car-3-ene, 47.4% (-)-sabinene, 35.2% terpinolene Picea sitchensis
4.2.3.107 G595A/L596F/F599L shift in product profile, mutant produces 5.4% (+)-car-3-ene, 29.0% (-)-sabinene, 26.4% terpinolene Picea sitchensis
4.2.3.107 L596F shift in product profile, mutant produces 12.3% (+)-car-3-ene, 37.4% (-)-sabinene, 35.4% terpinolene Picea sitchensis
4.2.3.107 L596F shift in product profile, mutant produces 5% (+)-car-3-ene, 20.9% (-)-sabinene, 53.5% terpinolene Picea sitchensis
4.2.3.107 L596F shift in product profile, mutant produces 9.2% (+)-car-3-ene, 20.2% (-)-sabinene, 32.2% terpinolene Picea sitchensis
4.2.3.107 additional information construction of a series of domain swaps and site-directed substitutions between (-)-sabinene synthase and (+)-3-carene synthase isoforms to explore which regions and specific amino acids of these enzymes affect the differences of their product profiles. The amino acid in position 596 is critical for product profiles dominated by (+)-3-carene in (+)-3-carene synthase isoforms. A leucine in this position promotes formation of (+)-3-carene, whereas phenylalanine promotes (-)-sabinene Picea sitchensis
4.2.3.109 A595G shift in product profile, mutant produces 2.4% (+)-car-3-ene, 39% (-)-sabinene, 33.3% terpinolene Picea sitchensis
4.2.3.109 A595G/F596L/L599F shift in product profile, mutant produces 42.3% (+)-car-3-ene, 7.3% (-)-sabinene, 20.1% terpinolene Picea sitchensis
4.2.3.109 F596E shift in product profile, mutant produces 0.5% (+)-car-3-ene, 10.2% (-)-sabinene, 5.7% terpinolene, 70.9% limonene Picea sitchensis
4.2.3.109 F596G shift in product profile, mutant produces 10.0% (+)-car-3-ene, 11.2% (-)-sabinene, 17.3% terpinolene, 27.0% myrcene, 17.0% limonene Picea sitchensis
4.2.3.109 F596H shift in product profile, mutant produces 2.1% (+)-car-3-ene, 46.5% (-)-sabinene, 27.1% terpinolene Picea sitchensis
4.2.3.109 F596L shift in product profile, mutant produces 28.6% (+)-car-3-ene, 42.8% (-)-sabinene, 36.4% terpinolene Picea sitchensis
4.2.3.109 F596R inactive Picea sitchensis
4.2.3.109 L599F shift in product profile, mutant produces 1.5% (+)-car-3-ene, 20.2% (-)-sabinene, 32.2% terpinolene Picea sitchensis
4.2.3.109 additional information construction of a series of domain swaps and site-directed substitutions between (-)-sabinene synthase and (+)-3-carene synthase isoforms to explore which regions and specific amino acids of these enzymes affect the differences of their product profiles. The amino acid in position 596 is critical for product profiles dominated by or (-)-sabinene. A leucine in this position promotes formation of (+)-3-carene, whereas phenylalanine promotes (-)-sabinene Picea sitchensis
4.2.3.109 S589A shift in product profile, mutant produces 1.2% (+)-car-3-ene, 42.9% (-)-sabinene, 35.5% terpinolene Picea sitchensis
4.2.3.113 A595G shift in product profile, mutant produces 2.4% (+)-car-3-ene, 39% (-)-sabinene, 33.3% terpinolene Picea sitchensis
4.2.3.113 A595G/F596L/L599F shift in product profile, mutant produces 42.3% (+)-car-3-ene, 7.3% (-)-sabinene, 20.1% terpinolene Picea sitchensis
4.2.3.113 F596E shift in product profile, mutant produces 0.5% (+)-car-3-ene, 10.2% (-)-sabinene, 5.7% terpinolene, 70.9% limonene Picea sitchensis
4.2.3.113 F596G shift in product profile, mutant produces 10.0% (+)-car-3-ene, 11.2% (-)-sabinene, 17.3% terpinolene, 27.0% myrcene, 17.0% limonene Picea sitchensis
4.2.3.113 F596H shift in product profile, mutant produces 2.1% (+)-car-3-ene, 46.5% (-)-sabinene, 27.1% terpinolene Picea sitchensis
4.2.3.113 F596L shift in product profile, mutant produces 28.6% (+)-car-3-ene, 42.8% (-)-sabinene, 36.4% terpinolene Picea sitchensis
4.2.3.113 F596R inactive Picea sitchensis
4.2.3.113 G595A/L596F/F599L shift in product profile, mutant produces 1.8% (+)-car-3-ene, 23.3% (-)-sabinene, 55.2% terpinolene Picea sitchensis
4.2.3.113 G595A/L596F/F599L shift in product profile, mutant produces 4.7% (+)-car-3-ene, 47.4% (-)-sabinene, 35.2% terpinolene Picea sitchensis
4.2.3.113 G595A/L596F/F599L shift in product profile, mutant produces 5.4% (+)-car-3-ene, 29.0% (-)-sabinene, 26.4% terpinolene Picea sitchensis
4.2.3.113 L596F shift in product profile, mutant produces 12.3% (+)-car-3-ene, 37.4% (-)-sabinene, 35.4% terpinolene Picea sitchensis
4.2.3.113 L596F shift in product profile, mutant produces 5% (+)-car-3-ene, 20.9% (-)-sabinene, 53.5% terpinolene Picea sitchensis
4.2.3.113 L596F shift in product profile, mutant produces 9.2% (+)-car-3-ene, 20.2% (-)-sabinene, 32.2% terpinolene Picea sitchensis
4.2.3.113 L599F shift in product profile, mutant produces 1.5% (+)-car-3-ene, 20.2% (-)-sabinene, 32.2% terpinolene Picea sitchensis
4.2.3.113 additional information construction of a series of domain swaps and site-directed substitutions between (-)-sabinene synthase and (+)-3-carene synthase isoforms to explore which regions and specific amino acids of these enzymes affect the differences of their product profiles. The amino acid in position 596 is critical for product profiles dominated by (+)-3-carene in (+)-3-carene synthase isoforms. A leucine in this position promotes formation of (+)-3-carene, whereas phenylalanine promotes (-)-sabinene Picea sitchensis
4.2.3.113 additional information construction of a series of domain swaps and site-directed substitutions between (-)-sabinene synthase and (+)-3-carene synthase isoforms to explore which regions and specific amino acids of these enzymes affect the differences of their product profiles. The amino acid in position 596 is critical for product profiles dominated by or (-)-sabinene. A leucine in this position promotes formation of (+)-3-carene, whereas phenylalanine promotes (-)-sabinene Picea sitchensis
4.2.3.113 S589A shift in product profile, mutant produces 1.2% (+)-car-3-ene, 42.9% (-)-sabinene, 35.5% terpinolene Picea sitchensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.107 0.0153
-
 geranyl diphosphate mutant L596F, 30°C, pH not specified in the publication Picea sitchensis
4.2.3.107 0.0158
-
 geranyl diphosphate wild-type, 30°C, pH not specified in the publication Picea sitchensis
4.2.3.109 0.0202
-
 geranyl diphosphate mutant A595G/F596L/L599F, 30°C, pH not specified in the publication Picea sitchensis
4.2.3.109 0.0316
-
 geranyl diphosphate mutant F596L, 30°C, pH not specified in the publication Picea sitchensis
4.2.3.109 0.0357
-
 geranyl diphosphate wild-type, 30°C, pH not specified in the publication Picea sitchensis
4.2.3.113 0.0153
-
 geranyl diphosphate mutant L596F, 30°C, pH not specified in the publication Picea sitchensis
4.2.3.113 0.0158
-
 geranyl diphosphate wild-type, 30°C, pH not specified in the publication Picea sitchensis
4.2.3.113 0.0202
-
 geranyl diphosphate mutant A595G/F596L/L599F, 30°C, pH not specified in the publication Picea sitchensis
4.2.3.113 0.0316
-
 geranyl diphosphate mutant F596L, 30°C, pH not specified in the publication Picea sitchensis
4.2.3.113 0.0357
-
 geranyl diphosphate wild-type, 30°C, pH not specified in the publication Picea sitchensis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.3.107 chloroplast
-
 Picea sitchensis 9507
-
4.2.3.109 chloroplast
-
 Picea sitchensis 9507
-
4.2.3.113 chloroplast
-
 Picea sitchensis 9507
-

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.107 Picea sitchensis F1CKI6 isoform TPS-3car1
-
4.2.3.107 Picea sitchensis F1CKI8 isoform TPS-3car2
-
4.2.3.107 Picea sitchensis F1CKI9 isoform TPS-3car3
-
4.2.3.109 Picea sitchensis
-
-
-
4.2.3.113 Picea sitchensis F1CKI6 isoform TPS-3car1
-
4.2.3.113 Picea sitchensis F1CKI8 isoform TPS-3car2
-
4.2.3.113 Picea sitchensis F1CKI9 isoform TPS-3car3
-
4.2.3.113 Picea sitchensis F1CKJ1 isoform TPS-sab
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.107 geranyl diphosphate
-
 Picea sitchensis (+)-car-3-ene + diphosphate wild-type produces 46.2% (+)-car-3-ene, 8.8% (-)-sabinene, 29.7% terpinolene ?
4.2.3.107 geranyl diphosphate
-
 Picea sitchensis (+)-car-3-ene + diphosphate wild-type produces 49% (+)-car-3-ene, 8.7% (-)-sabinene, 24.7% terpinolene ?
4.2.3.107 geranyl diphosphate
-
 Picea sitchensis (+)-car-3-ene + diphosphate wild-type produces 67.5% (+)-car-3-ene, 6.9% (-)-sabinene, 15.4% terpinolene ?
4.2.3.109 geranyl diphosphate
-
 Picea sitchensis (-)-sabinene + diphosphate wild-type produces 1.3% (+)-car-3-ene, 44.7% (-)-sabinene, 35.9% terpinolene ?
4.2.3.113 2 geranyl diphosphate
-
 Picea sitchensis (+)-car-3-ene + terpinolene + 2 diphosphate wild-type produces 46.2% (+)-car-3-ene, 8.8% (-)-sabinene, 29.7% terpinolene ?
4.2.3.113 2 geranyl diphosphate
-
 Picea sitchensis (+)-car-3-ene + terpinolene + 2 diphosphate wild-type produces 67.5% (+)-car-3-ene, 6.9% (-)-sabinene, 15.4% terpinolene ?
4.2.3.113 2 geranyl diphosphate
-
 Picea sitchensis (-)-sabinene + terpinolene + 2 diphosphate wild-type produces 1.3% (+)-car-3-ene, 44.7% (-)-sabinene, 35.9% terpinolene ?
4.2.3.113 geranyl diphosphate
-
 Picea sitchensis terpinolene + diphosphate wild-type produces 49% (+)-car-3-ene, 8.7% (-)-sabinene, 24.7% terpinolene ?

Synonyms

EC Number Synonyms Comment Organism
4.2.3.107 (+)-3-carene synthase
-
 Picea sitchensis
4.2.3.107 TPS-3car1
-
 Picea sitchensis
4.2.3.107 TPS-3car2
-
 Picea sitchensis
4.2.3.107 TPS-3car3
-
 Picea sitchensis
4.2.3.109 TPS-sab
-
 Picea sitchensis
4.2.3.113 (+)-3-carene synthase
-
 Picea sitchensis
4.2.3.113 TPS-3car1
-
 Picea sitchensis
4.2.3.113 TPS-3car2
-
 Picea sitchensis
4.2.3.113 TPS-3car3
-
 Picea sitchensis
4.2.3.113 TPS-sab
-
 Picea sitchensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.3.107 0.098
-
 geranyl diphosphate wild-type, 30°C, pH not specified in the publication Picea sitchensis
4.2.3.107 0.643
-
 geranyl diphosphate mutant L596F, 30°C, pH not specified in the publication Picea sitchensis
4.2.3.109 0.0352
-
 geranyl diphosphate mutant A595G/F596L/L599F, 30°C, pH not specified in the publication Picea sitchensis
4.2.3.109 0.0825
-
 geranyl diphosphate mutant F596L, 30°C, pH not specified in the publication Picea sitchensis
4.2.3.109 0.545
-
 geranyl diphosphate wild-type, 30°C, pH not specified in the publication Picea sitchensis
4.2.3.113 0.0352
-
 geranyl diphosphate mutant A595G/F596L/L599F, 30°C, pH not specified in the publication Picea sitchensis
4.2.3.113 0.0825
-
 geranyl diphosphate mutant F596L, 30°C, pH not specified in the publication Picea sitchensis
4.2.3.113 0.098
-
 geranyl diphosphate wild-type, 30°C, pH not specified in the publication Picea sitchensis
4.2.3.113 0.545
-
 geranyl diphosphate wild-type, 30°C, pH not specified in the publication Picea sitchensis
4.2.3.113 0.643
-
 geranyl diphosphate mutant L596F, 30°C, pH not specified in the publication Picea sitchensis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.3.107 0.0062
-
 geranyl diphosphate wild-type, 30°C, pH not specified in the publication Picea sitchensis
4.2.3.107 0.042
-
 geranyl diphosphate mutant L596F, 30°C, pH not specified in the publication Picea sitchensis
4.2.3.109 0.0017
-
 geranyl diphosphate mutant A595G/F596L/L599F, 30°C, pH not specified in the publication Picea sitchensis
4.2.3.109 0.0027
-
 geranyl diphosphate mutant F596L, 30°C, pH not specified in the publication Picea sitchensis
4.2.3.109 0.0153
-
 geranyl diphosphate wild-type, 30°C, pH not specified in the publication Picea sitchensis
4.2.3.113 0.0017
-
 geranyl diphosphate mutant A595G/F596L/L599F, 30°C, pH not specified in the publication Picea sitchensis
4.2.3.113 0.0027
-
 geranyl diphosphate mutant F596L, 30°C, pH not specified in the publication Picea sitchensis
4.2.3.113 0.0062
-
 geranyl diphosphate wild-type, 30°C, pH not specified in the publication Picea sitchensis
4.2.3.113 0.0153
-
 geranyl diphosphate wild-type, 30°C, pH not specified in the publication Picea sitchensis
4.2.3.113 0.042
-
 geranyl diphosphate mutant L596F, 30°C, pH not specified in the publication Picea sitchensis