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Literature summary extracted from

  • Rajavel, M.; Mitra, A.; Gopal, B.
    Role of Bacillus subtilis BacB in the synthesis of bacilysin (2009), J. Biol. Chem., 284, 31882-31892.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.3.19
-
 Bacillus subtilis
5.3.3.19 expression in Escherichia coli Bacillus subtilis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.3.3.19
-
 Bacillus subtilis

Protein Variants

EC Number Protein Variants Comment Organism
5.3.3.19 K107A significant decrease in catalytic activity when compared with the native enzyme Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.3.19 0.423
-
 3-[(4S)-4-hydroxycyclohexa-1,5-dien-1-yl]-2-oxopropanoate pH 8.1, 37°C Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.3.3.19 additional information the protein is a bi-cupin, with two putative active sites each containing a bound metal ion Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
5.3.3.19 Bacillus subtilis
-
-
-
5.3.3.19 Bacillus subtilis P39639
-
-
5.3.3.19 Bacillus subtilis 168 P39639
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.3.19
-
 Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.3.19 3-[(4R)-4-hydroxycyclohexa-1,5-dien-1-yl]-2-oxopropanoate
-
 Bacillus subtilis (3Z)-3-[(4R)-4-hydroxycyclohex-2-en-1-ylidene]-2-oxopropanoate
-
 ?
5.3.3.19 3-[(4R)-4-hydroxycyclohexa-1,5-dien-1-yl]-2-oxopropanoate
-
 Bacillus subtilis 168 (3Z)-3-[(4R)-4-hydroxycyclohex-2-en-1-ylidene]-2-oxopropanoate
-
 ?
5.3.3.19 3-[(4S)-4-hydroxycyclohexa-1,5-dien-1-yl]-2-oxopropanoate
-
 Bacillus subtilis (3E)-3-[(4S)-4-hydroxycyclohex-2-en-1-ylidene]-2-oxopropanoate
-
 ?
5.3.3.19 3-[(4S)-4-hydroxycyclohexa-1,5-dien-1-yl]-2-oxopropanoate
-
 Bacillus subtilis 168 (3E)-3-[(4S)-4-hydroxycyclohex-2-en-1-ylidene]-2-oxopropanoate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.3.3.19 BacB
-
 Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.3.19 22
-
 assay at Bacillus subtilis
5.3.3.19 37
-
 assay at Bacillus subtilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.3.19 24.52
-
 3-[(4S)-4-hydroxycyclohexa-1,5-dien-1-yl]-2-oxopropanoate pH 8.1, 37°C Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.3.19 8.1
-
 assay at Bacillus subtilis

General Information

EC Number General Information Comment Organism
5.3.3.19 physiological function the enzyme is involved in the biosynthesis of the dipeptide antibiotic bacilysin Bacillus subtilis