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Literature summary extracted from

  • Rybniker, J.; Krumbach, K.; van Gumpel, E.; Plum, G.; Eggeling, L.; Hartmann, P.
    The cytotoxic early protein 77 of mycobacteriophage L5 interacts with MSMEG_3532, an L-serine dehydratase of Mycobacterium smegmatis (2011), J. Basic Microbiol., 51, 515-522.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.1.17 expressed in Escherichia coli as a His-tagged fusion protein Mycolicibacterium smegmatis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.1.17 0.00258
-
 L-serine pH 7, temperature not specified in the publication, Vmax: 11.7/min/mg Mycolicibacterium smegmatis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.1.17 35680
-
 His-tagged fusion protein, calculated from cDNA Mycolicibacterium smegmatis
4.3.1.17 57000
-
 gel filtration, dimer Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.3.1.17 additional information Mycolicibacterium smegmatis cytotoxic early protein 77 of mycobacteriophage L5 interacts with MSMEG_3532 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.17 Mycolicibacterium smegmatis A0QY48
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.1.17 using Ni-NTA chromatography Mycolicibacterium smegmatis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.17 L-serine
-
 Mycolicibacterium smegmatis pyruvate + NH3
-
 ?
4.3.1.17 additional information cytotoxic early protein 77 of mycobacteriophage L5 interacts with MSMEG_3532 Mycolicibacterium smegmatis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.3.1.17 homodimer
-
 Mycolicibacterium smegmatis

Synonyms

EC Number Synonyms Comment Organism
4.3.1.17 MSMEG_3532
-
 Mycolicibacterium smegmatis
4.3.1.17 SdhA
-
 Mycolicibacterium smegmatis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.1.17 8.2
-
 assay at Mycolicibacterium smegmatis

Cofactor

EC Number Cofactor Comment Organism Structure
4.3.1.17 pyridoxal 5'-phosphate
-
 Mycolicibacterium smegmatis

General Information

EC Number General Information Comment Organism
4.3.1.17 physiological function cytotoxic early protein 77 of mycobacteriophage L5 interacts with MSMEG_3532 Mycolicibacterium smegmatis