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Literature summary extracted from

  • Pride, A.C.; Guan, Z.; Trent, M.S.
    Characterization of the Vibrio cholerae VolA surface-exposed lipoprotein lysophospholipase (2014), J. Bacteriol., 196, 1619-1626.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.5 EGTA EGTA by itself or in combination with EDTA causes a slight increase in activity Vibrio cholerae serotype O1

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.5 expressed in Escherichia coli BL21Star (DE3) cells Vibrio cholerae serotype O1

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.5 S551A inactive Vibrio cholerae serotype O1
3.1.1.5 S551T inactive Vibrio cholerae serotype O1

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.5 additional information the addition of EDTA alone had no effect on the lipase activity Vibrio cholerae serotype O1

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.5 cell surface
-
 Vibrio cholerae serotype O1 9986
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.5 additional information VolA is a metal-independent enzyme Vibrio cholerae serotype O1

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.5 81600
-
 x * 81600, SDS-PAGE Vibrio cholerae serotype O1

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.5 2-lysophosphatidylcholine + H2O Vibrio cholerae serotype O1
-
 glycerophosphocholine + a carboxylate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.5 Vibrio cholerae serotype O1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.5 Ni-Sepharose 6 Fast Flow column chromatography and Superdex 200 gel filtration Vibrio cholerae serotype O1

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.5 0.404
-
 purified enzyme, at pH 8.0 and 37°C Vibrio cholerae serotype O1

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.5 1-palmitoyl-sn-glycero-3-phosphoethanolamine + H2O
-
 Vibrio cholerae serotype O1 palmitic acid + glycero-3-phosphoethanolamine
-
 ?
3.1.1.5 2-lysophosphatidylcholine + H2O
-
 Vibrio cholerae serotype O1 glycerophosphocholine + a carboxylate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.5 ? x * 81600, SDS-PAGE Vibrio cholerae serotype O1

Synonyms

EC Number Synonyms Comment Organism
3.1.1.5 VolA
-
 Vibrio cholerae serotype O1

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.5 37
-
-
 Vibrio cholerae serotype O1

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.5 8
-
-
 Vibrio cholerae serotype O1

General Information

EC Number General Information Comment Organism
3.1.1.5 physiological function the lipase activity of surface-exposed VolA contributes to the success of Vibrio cholerae, improving the overall adaptation and survival of the organism in different environments Vibrio cholerae serotype O1