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Literature summary extracted from

  • Vincenzi, S.; Bierma, J.; Wickramasekara, S.I.; Curioni, A.; Gazzola, D.; Bakalinsky, A.T.
    Characterization of a grape class IV chitinase (2014), J. Agric. Food Chem., 62, 5660-5668.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.14 gene VvChi4D, DNA and amino acid sequence determination and analysis Vitis vinifera

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.14 31000
-
x * 31000, SDS-PAGE Vitis vinifera

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.14 chitin + H2O Vitis vinifera chitinases are glycosyl hydrolases that catalyze the degradation of chitin, a beta-1,4-linked polymer of N-acetylglucosamine N,N'-diacetylchitobiose + ?
-
?
3.2.1.14 chitin + H2O Vitis vinifera Manzoni Bianco chitinases are glycosyl hydrolases that catalyze the degradation of chitin, a beta-1,4-linked polymer of N-acetylglucosamine N,N'-diacetylchitobiose + ?
-
?
3.2.1.14 N-acetylglucosamine oligomers + H2O Vitis vinifera
-
N-acetylglucosamine
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Vitis vinifera Q7XAU6 gene VvChi4D
-
3.2.1.14 Vitis vinifera Manzoni Bianco Q7XAU6 gene VvChi4D
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.14 native enzyme from filtered grape juice in a procedure including cation exchange chromatography and dialysis Vitis vinifera

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.14 fruit grape juice, type IV grape chitinase Vitis vinifera
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 chitin + H2O crab shell chitin Vitis vinifera ?
-
?
3.2.1.14 chitin + H2O crab shell chitin Vitis vinifera Manzoni Bianco ?
-
?
3.2.1.14 chitin + H2O chitinases are glycosyl hydrolases that catalyze the degradation of chitin, a beta-1,4-linked polymer of N-acetylglucosamine Vitis vinifera N,N'-diacetylchitobiose + ?
-
?
3.2.1.14 chitin + H2O chitinases are glycosyl hydrolases that catalyze the degradation of chitin, a beta-1,4-linked polymer of N-acetylglucosamine Vitis vinifera Manzoni Bianco N,N'-diacetylchitobiose + ?
-
?
3.2.1.14 chitohexaose + H2O reaction via trimer formation Vitis vinifera 2 N-acetylglucosamine + 2 N,N'-diacetylchitobiose
-
?
3.2.1.14 chitopentaose + H2O reaction via trimer formation Vitis vinifera N-acetylglucosamine + 2 N,N'-diacetylchitobiose
-
?
3.2.1.14 colloidal chitin + H2O
-
Vitis vinifera N,N'-diacetylchitobiose + ? the molecule is not further processed, absence of 1,4-beta-N-acetylglucosaminidase activity ?
3.2.1.14 colloidal chitin + H2O
-
Vitis vinifera Manzoni Bianco N,N'-diacetylchitobiose + ? the molecule is not further processed, absence of 1,4-beta-N-acetylglucosaminidase activity ?
3.2.1.14 additional information type IV enzyme exhibits both endo- and exochitinase activities, absence of 1,4-beta-N-acetylglucosaminidase activity Vitis vinifera ?
-
?
3.2.1.14 additional information type IV enzyme exhibits both endo- and exochitinase activities, absence of 1,4-beta-N-acetylglucosaminidase activity Vitis vinifera Manzoni Bianco ?
-
?
3.2.1.14 N,N',N'',N'''-tetraacetylchitotetraose + H2O reaction via trimer formation Vitis vinifera 2 N-acetylglucosamine + N,N'-diacetylchitobiose
-
?
3.2.1.14 N,N',N''-triacetylchitotriose + H2O
-
Vitis vinifera N-acetyl-D-glucosamine + N,N'-diacetylchitobiose
-
?
3.2.1.14 N,N',N''-triacetylchitotriose + H2O
-
Vitis vinifera Manzoni Bianco N-acetyl-D-glucosamine + N,N'-diacetylchitobiose
-
?
3.2.1.14 N-acetylglucosamine oligomers + H2O
-
Vitis vinifera N-acetylglucosamine
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.14 ? x * 31000, SDS-PAGE Vitis vinifera
3.2.1.14 More tryptic peptide mapping and mass spectrometric analysis Vitis vinifera

Synonyms

EC Number Synonyms Comment Organism
3.2.1.14 class IV chitinase
-
Vitis vinifera

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.14 30
-
-
Vitis vinifera

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.14 10 60 activity range, at above 50°C, activity decreases drastically, profile overview Vitis vinifera

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.14 60
-
Tm at pH 4.0 Vitis vinifera
3.2.1.14 70
-
purified enzyme, pH 6.0, 1 h, 30% activity remaining Vitis vinifera

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 6
-
-
Vitis vinifera

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.14 5 6.5 narrow pH spectrum, sharp decrease in activity below and above Vitis vinifera

General Information

EC Number General Information Comment Organism
3.2.1.14 evolution the enzyme belongs to carbohydrate-binding module family 18 and glycoside hydrolase family GH19. Possibly the two proteins class IV chitinase and class IV endochitinase, UniProt IDs Q7XAU6 and O24530, are allelic variants encoded by the same gene Vitis vinifera
3.2.1.14 additional information at a concentration and pH expected in ripe grapes, no inhibition of wine yeast growth by the chitinase is observed Vitis vinifera