EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.73 | gene RmLic16A, DNA and amino acid sequence determination and analysis, sequence comparisons | Rhizomucor miehei |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.73 | additional information | - |
additional information | 2.0 mg/ml with barley beta-glucan and 1.4 mg/ml with lichenan at pH 5.5, 60°C | Rhizomucor miehei |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.2.1.73 | extracellular | - |
Rhizomucor miehei | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.73 | 31564 | - |
1 * 35400, SDS-PAGE, 1 * 31564, sequence calculation | Rhizomucor miehei |
3.2.1.73 | 33700 | - |
gel filtration | Rhizomucor miehei |
3.2.1.73 | 35400 | - |
1 * 35400, SDS-PAGE, 1 * 31564, sequence calculation | Rhizomucor miehei |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.73 | Rhizomucor miehei | H9BZ70 | gene RmLic16A | - |
3.2.1.73 | Rhizomucor miehei CAU432 | H9BZ70 | gene RmLic16A | - |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.2.1.73 | glycoprotein | the enzyme contains two possible N-glycosylation sites at 72 (NKT) and 275 (NPT), respectively | Rhizomucor miehei |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.73 | native enzyme 6.8fold by ammonium sulfate fractionation and two different steps of anion exchange chromatography, to homogeneity | Rhizomucor miehei |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.2.1.73 | additional information | high-level extracellular enzyme production of 6230 U/mL on oat flour at 3% w/v as a carbon source at 50 °C | Rhizomucor miehei | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.73 | 28820 | 33480 | purified native enzyme, substrate barley beta-glucan, pH 5.5, 60°C | Rhizomucor miehei |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.73 | barley beta-glucan + H2O | best substrate | Rhizomucor miehei | cellotriose + cellotetraose + cellobiose + D-glucose | oligosaccharides, mainly trisaccharide and tetrasaccharide | ? | |
3.2.1.73 | barley beta-glucan + H2O | best substrate | Rhizomucor miehei CAU432 | cellotriose + cellotetraose + cellobiose + D-glucose | oligosaccharides, mainly trisaccharide and tetrasaccharide | ? | |
3.2.1.73 | lichenan + H2O | 95.2% activity compared to barley beta-glucan | Rhizomucor miehei | cellotriose + ? | oligosaccharides, mainly trisaccharide | ? | |
3.2.1.73 | lichenan + H2O | 95.2% activity compared to barley beta-glucan | Rhizomucor miehei CAU432 | cellotriose + ? | oligosaccharides, mainly trisaccharide | ? | |
3.2.1.73 | additional information | thin layer chromatography product analysis, overview. Poor activity with laminarin, no activity with birchwood xylan, carboxymethyl cellulose, pullulan, soluble starch, locust bean gum, cellulose, Avicel, beta-1,3-glucan, 4-nitrophenyl-beta-D-glucopyranoside, 4-nitrophenyl-beta-D-xylopyranoside, and 4-nitrophenyl-beta-D-galactopyranoside, substrate specificity, overview | Rhizomucor miehei | ? | - |
? | |
3.2.1.73 | additional information | thin layer chromatography product analysis, overview. Poor activity with laminarin, no activity with birchwood xylan, carboxymethyl cellulose, pullulan, soluble starch, locust bean gum, cellulose, Avicel, beta-1,3-glucan, 4-nitrophenyl-beta-D-glucopyranoside, 4-nitrophenyl-beta-D-xylopyranoside, and 4-nitrophenyl-beta-D-galactopyranoside, substrate specificity, overview | Rhizomucor miehei CAU432 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.73 | monomer | 1 * 35400, SDS-PAGE, 1 * 31564, sequence calculation | Rhizomucor miehei |
3.2.1.73 | More | peptide mapping of native enzyme | Rhizomucor miehei |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.73 | beta-1,3-1,4-glucanase | - |
Rhizomucor miehei |
3.2.1.73 | endo-beta-1,3-1,4-glucanase | - |
Rhizomucor miehei |
3.2.1.73 | Lichenase | - |
Rhizomucor miehei |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.73 | 60 | - |
- |
Rhizomucor miehei |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.73 | 40 | 65 | activity range, profile overview | Rhizomucor miehei |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.73 | 60 | - |
purified native enzyme, no loss in activity at pH 5.5 for 30 min, half-life is 225 min | Rhizomucor miehei |
3.2.1.73 | 70 | - |
purified native enzyme, pH 5.5, half-life is 101 min | Rhizomucor miehei |
3.2.1.73 | 75 | - |
purified native enzyme, pH 5.5, half-life is 64 min | Rhizomucor miehei |
3.2.1.73 | 80 | - |
purified native enzyme, pH 5.5, half-life is 53 min, 80% activity remaining after 30 min | Rhizomucor miehei |
3.2.1.73 | 85 | - |
purified native enzyme, pH 5.5, half-life is 27 min, inactivation after 120 min | Rhizomucor miehei |
3.2.1.73 | 90 | - |
purified native enzyme, pH 5.5, 60% activity remaining after 30 min | Rhizomucor miehei |
3.2.1.73 | 100 | - |
purified native enzyme, pH 5.5, 40% activity remaining after 30 min | Rhizomucor miehei |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.73 | 5.5 | - |
- |
Rhizomucor miehei |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.73 | 3.5 | 7.5 | activity range, profile overview | Rhizomucor miehei |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.73 | 5 | 7.5 | purified native enzyme, retains 90% of its activity at 60°C for 30 min | Rhizomucor miehei |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.2.1.73 | Rhizomucor miehei | sequence calculation | - |
3.9 |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.2.1.73 | evolution | the enzyme is a member of the glycosylhydrolase faamily GH16 | Rhizomucor miehei |