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Literature summary extracted from

  • Martinez, V.; Dinjaski, N.; de Eugenio, L.I.; de la Pena, F.; Prieto, M.A.
    Cell system engineering to produce extracellular polyhydroxyalkanoate depolymerase with targeted applications (2014), Int. J. Biol. Macromol., 71, 28-33.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.76 recombinant expression of the extracellular enzyme in different hosts, e.g. Escherichia coli strains MC4100 (pUC18) and MC4100 (pLJ1) , and Pseudomonas putida strains KT2442, KT42FadB (pIZ1016), or KT42FadB(pIZPZ), method optimization and evaluation, the highest enzyme activity is obtained using Pseudomonas putida strain KT2442 fadB mutant Pseudomonas fluorescens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.76 extracellular
-
 Pseudomonas fluorescens
-
-

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.76 Pseudomonas fluorescens
-
-
-
3.1.1.76 Pseudomonas fluorescens GK13
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.76 additional information the recombinant enzyme degrades previously biosynthesized polyhydroxyalkanotes to obtain 3-hydroxyalkanoic acids, it is also active on functionalized medium-chain-length-polyhydroxyalkanotes, i.e. PHACOS, harboring thioester groups in the side chai Pseudomonas fluorescens ?
-
 ?
3.1.1.76 additional information the recombinant enzyme degrades previously biosynthesized polyhydroxyalkanotes to obtain 3-hydroxyalkanoic acids, it is also active on functionalized medium-chain-length-polyhydroxyalkanotes, i.e. PHACOS, harboring thioester groups in the side chai Pseudomonas fluorescens GK13 ?
-
 ?
3.1.1.76 polyhydroxyoctanoate-co-hexanoate + H2O
-
 Pseudomonas fluorescens ?
-
 ?
3.1.1.76 polyhydroxyoctanoate-co-hexanoate + H2O
-
 Pseudomonas fluorescens GK13 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.76 PhaZGK13
-
 Pseudomonas fluorescens
3.1.1.76 polyhydroxyalkanoate depolymerase
-
 Pseudomonas fluorescens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.76 37
-
 assay at Pseudomonas fluorescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.76 8
-
 assay at Pseudomonas fluorescens

General Information

EC Number General Information Comment Organism
3.1.1.76 additional information hydrolyzed functionalized mcl-polyhydroxyalkanotes PHACOS show greater potential to inhibit Staphylococcus aureus growth when compared to that of degradation products of non functionalized polyhydroxyoctanoate-co-hexanoate P(HO-co-HH) Pseudomonas fluorescens
3.1.1.76 physiological function influence of an active beta-oxidationpathway on the extracellular degradation of polyhydroxyalkanoates, overview Pseudomonas fluorescens