EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.1.29 | expressed in Escherichia coli BL21(lambdaDE3) cells | Mycolicibacterium smegmatis |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.1.1.29 | hanging drop vapor diffusion method, using 30% (w/v) PEG-1500 and 10% (v/v) isopropanol in 100 mM HEPES buffer, pH 6.5 | Mycolicibacterium smegmatis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.1.29 | 21000 | - |
1 * 21000, SDS-PAGE | Mycolicibacterium smegmatis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.29 | N-substituted aminoacyl-tRNA + H2O | Mycolicibacterium smegmatis | - |
N-substituted amino acid + tRNA | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.29 | Mycolicibacterium smegmatis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.1.29 | Ni-NTA column chromatography and Superdex G-75 gel filtration | Mycolicibacterium smegmatis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.29 | N-substituted aminoacyl-tRNA + H2O | - |
Mycolicibacterium smegmatis | N-substituted amino acid + tRNA | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.1.29 | monomer | 1 * 21000, SDS-PAGE | Mycolicibacterium smegmatis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.1.29 | peptidyl-tRNA hydrolase | - |
Mycolicibacterium smegmatis |
3.1.1.29 | PTH | - |
Mycolicibacterium smegmatis |