Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Nieter, A.; Haase-Aschoff, P.; Linke, D.; Nimtz, M.; Berger, R.G.
    A halotolerant type A feruloyl esterase from Pleurotus eryngii (2014), Fungal Biol., 118, 348-357.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.73 acetone strong inhibition Pleurotus eryngii
3.1.1.73 acetonitrile strong inhibition Pleurotus eryngii
3.1.1.73 Co2+ inhibitory at 5 mM Pleurotus eryngii
3.1.1.73 diethyl ether strong inhibition Pleurotus eryngii
3.1.1.73 DMF strong inhibition Pleurotus eryngii
3.1.1.73 DMSO strong inhibition Pleurotus eryngii
3.1.1.73 ethanol strong inhibition Pleurotus eryngii
3.1.1.73 Hg2+ inhibitory at 5 mM Pleurotus eryngii
3.1.1.73 isobutyl alcohol strong inhibition Pleurotus eryngii
3.1.1.73 Li+ inhibitory at 5 mM Pleurotus eryngii
3.1.1.73 methanol complete inhibition Pleurotus eryngii
3.1.1.73 Mg2+ inhibitory at 5 mM Pleurotus eryngii
3.1.1.73 Mn2+ inhibitory at 5 mM Pleurotus eryngii
3.1.1.73 additional information no inhibition by EDTA Pleurotus eryngii
3.1.1.73 n-heptane
-
 Pleurotus eryngii
3.1.1.73 n-hexane
-
 Pleurotus eryngii
3.1.1.73 PMSF 50-100% inhibition at 1 mM dependent on the buffer system Pleurotus eryngii
3.1.1.73 SDS 95% inhibition at 5 mM Pleurotus eryngii
3.1.1.73 tert-butyl methyl ether strong inhibition Pleurotus eryngii
3.1.1.73 Toluene strong inhibition Pleurotus eryngii
3.1.1.73 Zn2+ inhibitory at 5 mM Pleurotus eryngii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.73 0.15
-
 methyl ferulate pH 5.0, 37°C Pleurotus eryngii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.73 extracellular
-
 Pleurotus eryngii
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.73 Ca2+ addition of 50 mM increases the initial activity by 10-20% Pleurotus eryngii
3.1.1.73 K+ addition of 50 mM increases the initial activity by 10-20% Pleurotus eryngii
3.1.1.73 Li+ addition of 50 mM increases the initial activity by 10-20% Pleurotus eryngii
3.1.1.73 Mg2+ addition of 50 mM increases the initial activity by 10-20% Pleurotus eryngii
3.1.1.73 additional information no or poor effects by Fe2+, Fe3+, Cu2+, Ni2+, Ca2+, K+ at 5 mM Pleurotus eryngii
3.1.1.73 NaCl in the presence of 3 M activity of the enzyme increases by 28% Pleurotus eryngii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.73 59200
-
 1 * 67000, SDS-PAGE, 1 * 59200, about, sequence calculation Pleurotus eryngii
3.1.1.73 67000
-
 gel filtration Pleurotus eryngii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.73 additional information Pleurotus eryngii the enzyme alone releases only little ferulic acid from destarched wheat bran, whereas after addition of Trichoderma viride xylanase the concentration of ferulic acid increases more than 20fold ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.73 Pleurotus eryngii V6BP73 type A feruloyl esterase
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.73 glycoprotein four potential N-glycosylation sites at positions 132, 360, 396, and 521 are identified and the presence of at least one glycopeptide with mainly high mannose type glycans by ESI-MS/MS confirmed Pleurotus eryngii

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.73 native extracellular enzyme by ultrafiltration, cation exchange and hydrophobic interaction chromatography, and gel filtration to homogeneity Pleurotus eryngii

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.73 ethyl 4-hydroxybenzoate + H2O low activity Pleurotus eryngii ethanol + 4-hydroxybenzoate
-
 ?
3.1.1.73 ethyl ferulate + H2O
-
 Pleurotus eryngii ferulate + ethanol
-
 ?
3.1.1.73 gallic acid + H2O low activity Pleurotus eryngii ?
-
 ?
3.1.1.73 methyl 4-coumarate + H2O
-
 Pleurotus eryngii 4-coumarate + methanol
-
 ?
3.1.1.73 methyl 4-hydroxybenzoate + H2O low activity Pleurotus eryngii methanol + 4-hydroxybenzoate
-
 ?
3.1.1.73 methyl ferulate + H2O
-
 Pleurotus eryngii ferulate + methanol
-
 ?
3.1.1.73 methyl sinapinate + H2O
-
 Pleurotus eryngii sinapate + methanol
-
 ?
3.1.1.73 additional information the enzyme alone releases only little ferulic acid from destarched wheat bran, whereas after addition of Trichoderma viride xylanase the concentration of ferulic acid increases more than 20fold Pleurotus eryngii ?
-
 ?
3.1.1.73 propyl 4-hydroxybenzoate + H2O low activity Pleurotus eryngii propanol + 4-hydroxybenzoate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.73 monomer 1 * 67000, SDS-PAGE, 1 * 59200, about, sequence calculation Pleurotus eryngii
3.1.1.73 More peptide mapping, overview Pleurotus eryngii

Synonyms

EC Number Synonyms Comment Organism
3.1.1.73 FAE
-
 Pleurotus eryngii
3.1.1.73 PeFaeA
-
 Pleurotus eryngii
3.1.1.73 type A FAE
-
 Pleurotus eryngii
3.1.1.73 type A feruloyl esterase
-
 Pleurotus eryngii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.73 50
-
-
 Pleurotus eryngii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.73 0.85
-
 methyl ferulate pH 5.0, 37°C Pleurotus eryngii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.73 5
-
-
 Pleurotus eryngii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.73 3.5 7 activity range with 50% of maximal activity within this range, rapid decrease in activity above pH 8.0 Pleurotus eryngii

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.73 Pleurotus eryngii isoelectric focussing
-
 5.2

General Information

EC Number General Information Comment Organism
3.1.1.73 additional information the catalytic triad of Ser-Glu-His is unique among feruloyl esterases, the enzyme has a G-X-S-X-G motif Pleurotus eryngii