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Literature summary extracted from

  • Jiang, X.; Jia, Q.; Chen, L.; Chen, Q.; Yang, Q.
    Recombinant expression and inhibition mechanism analysis of pectin methylesterase from Aspergillus flavus (2014), FEMS Microbiol. Lett., 355, 12-19.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.11 expressed in Pichia pastoris Aspergillus flavus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.11 (-)-epigallocatechin 82% residual activity at 20 mg/ml Aspergillus flavus
3.1.1.11 catechinhydrate 83% residual activity at 20 mg/ml Aspergillus flavus
3.1.1.11 epigallocatechin gallate 47% residual activity at 20 mg/ml Aspergillus flavus
3.1.1.11 gallic acid 80% residual activity at 20 mg/ml Aspergillus flavus
3.1.1.11 additional information 10 mg/ml sucrose and 10 mg/ml coumaric acid have no effect on enzyme activity Aspergillus flavus
3.1.1.11 SDS 78% residual activity at 10 mg/ml SDS Aspergillus flavus
3.1.1.11 Tannic acid 10 mg/ml tannic acid completely inhibits the enzyme Aspergillus flavus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.11 40000
-
 x * 40000, SDS-PAGE Aspergillus flavus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.11 pectin + H2O Aspergillus flavus
-
 methanol + pectate
-
 ?
3.1.1.11 pectin + H2O Aspergillus flavus NRRL3357
-
 methanol + pectate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.11 Aspergillus flavus
-
-
-
3.1.1.11 Aspergillus flavus NRRL3357
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.11 ammonium sulfate precipitation, HisTrap column chromatography, and SOURCE 30Q column chromatography Aspergillus flavus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.11 5.7
-
 crude enzyme, at pH 5.0 and 30°C Aspergillus flavus
3.1.1.11 194
-
 after 34fold purification, at pH 5.0 and 30°C Aspergillus flavus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.11 pectin + H2O
-
 Aspergillus flavus methanol + pectate
-
 ?
3.1.1.11 pectin + H2O
-
 Aspergillus flavus NRRL3357 methanol + pectate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.11 ? x * 40000, SDS-PAGE Aspergillus flavus

Synonyms

EC Number Synonyms Comment Organism
3.1.1.11 pectin methylesterase
-
 Aspergillus flavus
3.1.1.11 PME
-
 Aspergillus flavus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.11 55
-
-
 Aspergillus flavus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.11 30 65 the enzyme activity is slightly increased at temperatures above 30°C, but peaks at 55°C. At temperatures above 55°C, the activity drops rapidly, and no activity is detected at 65°C Aspergillus flavus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.11 4.8
-
-
 Aspergillus flavus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.11 4.2 6
-
 Aspergillus flavus