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Literature summary extracted from

  • Dutta, A.; Bhattacharyya, S.; Dutta, D.; Das, A.K.
    Structural elucidation of the binding site and mode of inhibition of Li+ and Mg2+ in inositol monophosphatase (2014), FEBS J., 281, 5309-5324.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.3.25 hanging drop vapor diffusion method, using 0.2 M MgCl2, 0.1 M Na/HEPES pH 8.0, 18% (w/v) poly(ethyleneglycol) 3350 Staphylococcus aureus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.25 Li+ competitive inhibitor of the cofactor Mg2+ Staphylococcus aureus
3.1.3.25 Mg2+ uncompetitive inhibitor at high concentrations Staphylococcus aureus
3.1.3.25 myo-inositol non-competitive inhibition Staphylococcus aureus
3.1.3.25 PO43- competitive inhibition Staphylococcus aureus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.25 Mg2+ dependent on, contains three Mg2+ ions in the active site Staphylococcus aureus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.25 myo-inositol phosphate + H2O Staphylococcus aureus
-
 myo-inositol + phosphate
-
 ?
3.1.3.25 myo-inositol phosphate + H2O Staphylococcus aureus MSSA476
-
 myo-inositol + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.25 Staphylococcus aureus A0A1I9GET0
-
-
3.1.3.25 Staphylococcus aureus MSSA476 A0A1I9GET0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.25 Ni-NTA affinity column chromatography Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.25 myo-inositol phosphate + H2O
-
 Staphylococcus aureus myo-inositol + phosphate
-
 ?
3.1.3.25 myo-inositol phosphate + H2O
-
 Staphylococcus aureus MSSA476 myo-inositol + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.25 IMPase I
-
 Staphylococcus aureus
3.1.3.25 inositol monophosphatase
-
 Staphylococcus aureus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.3.25 81
-
 Li+ pH and temperature not specified in the publication Staphylococcus aureus
3.1.3.25 88
-
 Mg2+ pH and temperature not specified in the publication Staphylococcus aureus