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Literature summary extracted from

  • Permanasari, E.D.; Angkawidjaja, C.; Koga, Y.; Kanaya, S.
    Role of N-terminal extension of Bacillus stearothermophilus RNase H2 and C-terminal extension of Thermotoga maritima RNase H2 (2013), FEBS J., 280, 5065-5079.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.26.4 expressed in Escherichia coli MIC2067(DE3) cells Geobacillus stearothermophilus
3.1.26.4 expressed in Escherichia coli MIC2067(DE3) cells Thermotoga maritima

General Stability

EC Number General Stability Organism
3.1.26.4 the C-terminal extension of the enzyme functions as a substrate-binding domain and stabilizes the RNase H domain Thermotoga maritima
3.1.26.4 the N-terminal extension of the enzyme functions as a substrate-binding domain and stabilizes the RNase H domain Geobacillus stearothermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.26.4 Mg2+ the optimum concentration is 1 mM Thermotoga maritima
3.1.26.4 Mg2+ the optimum concentration is 10 mM Geobacillus stearothermophilus
3.1.26.4 Mn2+ the optimum concentration is 1 mM Thermotoga maritima
3.1.26.4 Mn2+ the optimum concentration is 10 mM Geobacillus stearothermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.26.4 26630
-
 1 * 26630, SDS-PAGE Thermotoga maritima
3.1.26.4 27000
-
 gel filtration Thermotoga maritima
3.1.26.4 28892
-
 1 * 28892, SDS-PAGE Geobacillus stearothermophilus
3.1.26.4 32000
-
 gel filtration Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.26.4 12 base pair RNA-DNA hybrid + H2O Geobacillus stearothermophilus
-
 ribonucleotide 5'-phosphomonoester + ?
-
 ?
3.1.26.4 12 base pair RNA-DNA hybrid + H2O Thermotoga maritima
-
 ribonucleotide 5'-phosphomonoester + ?
-
 ?
3.1.26.4 12 base pair RNA-DNA hybrid + H2O Thermotoga maritima DSM 3109
-
 ribonucleotide 5'-phosphomonoester + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.26.4 Geobacillus stearothermophilus
-
-
-
3.1.26.4 Thermotoga maritima Q9X017
-
-
3.1.26.4 Thermotoga maritima DSM 3109 Q9X017
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.26.4 HiTrap Q column chromatography, HiTrap heparin column chromatography, and Superdex 200 gel filtration Geobacillus stearothermophilus
3.1.26.4 HiTrap Q column chromatography, HiTrap heparin column chromatography, and Superdex 200 gel filtration Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.26.4 12 base pair RNA-DNA hybrid + H2O
-
 Geobacillus stearothermophilus ribonucleotide 5'-phosphomonoester + ?
-
 ?
3.1.26.4 12 base pair RNA-DNA hybrid + H2O
-
 Thermotoga maritima ribonucleotide 5'-phosphomonoester + ?
-
 ?
3.1.26.4 12 base pair RNA-DNA hybrid + H2O
-
 Thermotoga maritima DSM 3109 ribonucleotide 5'-phosphomonoester + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.26.4 monomer 1 * 26630, SDS-PAGE Thermotoga maritima
3.1.26.4 monomer 1 * 28892, SDS-PAGE Geobacillus stearothermophilus

Synonyms

EC Number Synonyms Comment Organism
3.1.26.4 RNase H2
-
 Geobacillus stearothermophilus
3.1.26.4 RNase H2
-
 Thermotoga maritima
3.1.26.4 RNH2
-
 Geobacillus stearothermophilus
3.1.26.4 RNH2
-
 Thermotoga maritima

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.26.4 53.1
-
 melting temperature Geobacillus stearothermophilus
3.1.26.4 66.1
-
 melting temperature Thermotoga maritima