EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
5.4.99.39 | Triton X-100 | at 0.05% highest activity of the enzyme | Euphorbia tirucalli |
EC Number | Cloned (Comment) | Organism |
---|---|---|
5.4.99.39 | expressed as a polyhistidine-tagged protein in Saccharomyces cerevisiae GIL77, which lacks the lanosterol synthase gene. Expression yield: 5-7 mg | Euphorbia tirucalli |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
5.4.99.39 | C486A | mutant shows 46% activity compared to wild-type (100%), Km similar to wild-type, kcat decreased | Euphorbia tirucalli |
5.4.99.39 | C564A | mutant shows 1.6% activity compared to wild-type (100%), Km increased compared to wild-type, kcat highly decreased | Euphorbia tirucalli |
5.4.99.39 | D485E | mutant shows no activity | Euphorbia tirucalli |
5.4.99.39 | D485N | mutant shows no activity | Euphorbia tirucalli |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
5.4.99.39 | (4-bromophenyl)[2-fluoro-4-([6-[methyl(prop-2-en-1-yl)amino]hexyl]oxy)phenyl]methanone | - |
Euphorbia tirucalli | |
5.4.99.39 | Iminosqualene | - |
Euphorbia tirucalli | |
5.4.99.39 | N,N-diethyl-2-[[(3beta)-17-methylideneandrost-5-en-3-yl]oxy]ethanamine | - |
Euphorbia tirucalli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.4.99.39 | 0.0338 | - |
(S)-2,3-oxidosqualene | pH 7, 30°C, wild-type | Euphorbia tirucalli | |
5.4.99.39 | 0.0348 | - |
(S)-2,3-oxidosqualene | pH 7, 30°C, mutant C486A | Euphorbia tirucalli | |
5.4.99.39 | 0.0511 | - |
(S)-2,3-oxidosqualene | pH 7, 30°C, mutant C564A | Euphorbia tirucalli |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
5.4.99.39 | 77000 | - |
SDS-PAGE | Euphorbia tirucalli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.4.99.39 | Euphorbia tirucalli | Q401R6 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.4.99.39 | using Ni-NTA chromatography | Euphorbia tirucalli |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
5.4.99.39 | 0.352 | - |
pH 7, 30°C | Euphorbia tirucalli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.4.99.39 | (S)-2,3-oxidosqualene | - |
Euphorbia tirucalli | beta-amyrin | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
5.4.99.39 | monomer | - |
Euphorbia tirucalli |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.4.99.39 | amyrin synthase | - |
Euphorbia tirucalli |
5.4.99.39 | EtAS | - |
Euphorbia tirucalli |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.4.99.39 | 30 | - |
- |
Euphorbia tirucalli |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.4.99.39 | 0.0000128 | - |
(S)-2,3-oxidosqualene | pH 7, 30°C, mutant C564A | Euphorbia tirucalli | |
5.4.99.39 | 0.34 | - |
(S)-2,3-oxidosqualene | pH 7, 30°C, mutant C486A | Euphorbia tirucalli | |
5.4.99.39 | 0.77 | - |
(S)-2,3-oxidosqualene | pH 7, 30°C, wild-type | Euphorbia tirucalli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
5.4.99.39 | 7 | - |
- |
Euphorbia tirucalli |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.4.99.39 | 0.00000698 | - |
(4-bromophenyl)[2-fluoro-4-([6-[methyl(prop-2-en-1-yl)amino]hexyl]oxy)phenyl]methanone | pH 7, 30°C | Euphorbia tirucalli | |
5.4.99.39 | 0.0000134 | - |
Iminosqualene | pH 7, 30°C | Euphorbia tirucalli | |
5.4.99.39 | 0.000102 | - |
N,N-diethyl-2-[[(3beta)-17-methylideneandrost-5-en-3-yl]oxy]ethanamine | pH 7, 30°C | Euphorbia tirucalli |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
5.4.99.39 | 0.0000107 | - |
pH 7, 30°C | Euphorbia tirucalli | (4-bromophenyl)[2-fluoro-4-([6-[methyl(prop-2-en-1-yl)amino]hexyl]oxy)phenyl]methanone | |
5.4.99.39 | 0.0000309 | - |
pH 7, 30°C | Euphorbia tirucalli | Iminosqualene | |
5.4.99.39 | 0.000142 | - |
pH 7, 30°C | Euphorbia tirucalli | N,N-diethyl-2-[[(3beta)-17-methylideneandrost-5-en-3-yl]oxy]ethanamine |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.4.99.39 | 0.0000025 | - |
(S)-2,3-oxidosqualene | pH 7, 30°C, mutant C564A | Euphorbia tirucalli | |
5.4.99.39 | 9.8 | - |
(S)-2,3-oxidosqualene | pH 7, 30°C, mutant C486A | Euphorbia tirucalli | |
5.4.99.39 | 22.83 | - |
(S)-2,3-oxidosqualene | pH 7, 30°C, wild-type | Euphorbia tirucalli |