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Literature summary extracted from

  • Andersen, R.W.; Leggio, L.L.; Hove-Jensen, B.; Kadziola, A.
    Structure of dimeric, recombinant Sulfolobus solfataricus phosphoribosyl diphosphate synthase: a bent dimer defining the adenine specificity of the substrate ATP (2015), Extremophiles, 19, 407-415.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.6.1 expression in Escherichia coli Saccharolobus solfataricus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.6.1 vapour diffusion sitting or hanging drop method. The protein is co-crystallised in the presence of AMP and D-ribose 5-phosphate, but in the electron density map of the active site only AMP and a sulphate ion are observed. Sulphate ion, reminiscent of the ammonium sulfate precipitation step of the purification, seems to bind tightly and, therefore, presumably occupies and blocks the D-ribose 5-phosphate binding site. The structure is determined at 2.8 A resolution Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.6.1 34000
-
 2 * 34000, SDS-PAGE Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.6.1 ATP + D-ribose 5-phosphate Saccharolobus solfataricus
-
 AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 ?
2.7.6.1 ATP + D-ribose 5-phosphate Saccharolobus solfataricus P2
-
 AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.6.1 Saccharolobus solfataricus Q97Z86
-
-
2.7.6.1 Saccharolobus solfataricus P2 Q97Z86
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.6.1
-
 Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.6.1 ATP + D-ribose 5-phosphate
-
 Saccharolobus solfataricus AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 ?
2.7.6.1 ATP + D-ribose 5-phosphate
-
 Saccharolobus solfataricus P2 AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.6.1 dimer 2 * 34000, SDS-PAGE Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
2.7.6.1 5-phosphoribosyl-1-alpha-diphosphate synthase
-
 Saccharolobus solfataricus
2.7.6.1 PRPP synthase
-
 Saccharolobus solfataricus
2.7.6.1 SSO1045 locus name Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.6.1 60
-
 assay at Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.6.1 7.6
-
 assay at Saccharolobus solfataricus