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Literature summary extracted from

  • Sholder, G.; Loechler, E.L.
    A method to accurately quantitate intensities of (32)P-DNA bands when multiple bands appear in a single lane of a gel is used to study dNTP insertion opposite a benzo[a]pyrene-dG adduct by Sulfolobus DNA polymerases Dpo4 and Dbh (2014), DNA Repair, 25, 97-103.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.7 additional information
-
 additional information kinetic study of dNTP primer-extension opposite a benzo[a]pyrene-N2-dG-adduct with four DNA polymerases, including Sulfolobus solfataricus Dpo4 and Sulfolobus acidocaldarius Dbh. Vmax/Km is similar for correct dCTP insertion with Dpo4 and Dbh. Compared to Dpo4, Dbh misinsertion is slower for dATP (about 20fold), dGTP (about 110fold) and dTTP (about 6fold), due to decreases in Vmax. These findings provide support that Dbh is in the same Y-Family DNA polymerase class as eukaryotic DNA polymerase kappa and bacterial DNA polymerase IV, which accurately bypass N2-dG adducts Sulfolobus acidocaldarius
2.7.7.7 additional information
-
 additional information kinetic study of dNTP primer-extension opposite a benzo[a]pyrene-N2-dG-adduct with four DNA polymerases, including Sulfolobus solfataricus Dpo4 and Sulfolobus acidocaldarius Dbh. Vmax/Km is similar for correct dCTP insertion with Dpo4 and Dbh. Compared to Dpo4, Dbh misinsertion is slower for dATP (about 20fold), dGTP (about 110fold) and dTTP (about 6fold), due to decreases in Vmax. These findings provide support that Dbh is in the same Y-Family DNA polymerase class as eukaryotic DNA polymerase kappa and bacterial DNA polymerase IV, which accurately bypass N2-dG adducts Saccharolobus solfataricus
2.7.7.7 0.65
-
 dCTP pH and temperature not specified in the publication Sulfolobus acidocaldarius
2.7.7.7 0.74
-
 dATP pH and temperature not specified in the publication Sulfolobus acidocaldarius
2.7.7.7 0.77
-
 dATP pH and temperature not specified in the publication Saccharolobus solfataricus
2.7.7.7 0.94
-
 dTTP pH and temperature not specified in the publication Saccharolobus solfataricus
2.7.7.7 1
-
 dTTP pH and temperature not specified in the publication Sulfolobus acidocaldarius
2.7.7.7 1.2
-
 dGTP pH and temperature not specified in the publication Saccharolobus solfataricus
2.7.7.7 1.4
-
 dGTP pH and temperature not specified in the publication Sulfolobus acidocaldarius
2.7.7.7 2.5
-
 dCTP pH and temperature not specified in the publication Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.7 Saccharolobus solfataricus
-
-
-
2.7.7.7 Sulfolobus acidocaldarius
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.7 dATP + DNAn
-
 Sulfolobus acidocaldarius diphosphate + DNAn+1
-
 ?
2.7.7.7 dATP + DNAn dNTP insertion opposite a benzo[a]pyrene-N2-dG-adduct Saccharolobus solfataricus diphosphate + DNAn+1
-
 ?
2.7.7.7 dCTP + DNAn
-
 Sulfolobus acidocaldarius diphosphate + DNAn+1
-
 ?
2.7.7.7 dCTP + DNAn dNTP insertion opposite a benzo[a]pyrene-N2-dG-adduct Saccharolobus solfataricus diphosphate + DNAn+1
-
 ?
2.7.7.7 dGTP + DNAn
-
 Sulfolobus acidocaldarius diphosphate + DNAn+1
-
 ?
2.7.7.7 dGTP + DNAn dNTP insertion opposite a benzo[a]pyrene-N2-dG-adduct Saccharolobus solfataricus diphosphate + DNAn+1
-
 ?
2.7.7.7 dTTP + DNAn
-
 Sulfolobus acidocaldarius diphosphate + DNAn+1
-
 ?
2.7.7.7 dTTP + DNAn dNTP insertion opposite a benzo[a]pyrene-N2-dG-adduct Saccharolobus solfataricus diphosphate + DNAn+1
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.7 DBH
-
 Sulfolobus acidocaldarius
2.7.7.7 Dpo4
-
 Saccharolobus solfataricus