BRENDA - Enzyme Database

Effect of cation-pi interactions and steric bulk on the catalytic action of oxidosqualene cyclase: a case study of Phe728 of beta-amyrin synthase from Euphorbia tirucalli L

Ito, R.; Hashimoto, I.; Masukawa, Y.; Hoshino, T.; Chemistry 19, 17150-17158 (2013)

Data extracted from this reference:

Engineering
EC Number
Protein Variants
Commentary
Organism
5.4.99.39
F728A
highly reduced activity compared to wild-type
Euphorbia tirucalli
5.4.99.39
F728H
highly reduced activity compared to wild-type
Euphorbia tirucalli
5.4.99.39
F728I
highly reduced activity compared to wild-type
Euphorbia tirucalli
5.4.99.39
F728M
highly reduced activity compared to wild-type
Euphorbia tirucalli
5.4.99.39
F728W
highly reduced activity compared to wild-type
Euphorbia tirucalli
5.4.99.39
F728Y
activity comparable to wild-type
Euphorbia tirucalli
Organism
EC Number
Organism
UniProt
Commentary
Textmining
5.4.99.39
Euphorbia tirucalli
Q401R6
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
5.4.99.39
(S)-2,3-oxidosqualene
-
729601
Euphorbia tirucalli
beta-amyrin
-
-
-
?
Synonyms
EC Number
Synonyms
Commentary
Organism
5.4.99.39
amyrin synthase
-
Euphorbia tirucalli
5.4.99.39
EtAS
-
Euphorbia tirucalli
Engineering (protein specific)
EC Number
Protein Variants
Commentary
Organism
5.4.99.39
F728A
highly reduced activity compared to wild-type
Euphorbia tirucalli
5.4.99.39
F728H
highly reduced activity compared to wild-type
Euphorbia tirucalli
5.4.99.39
F728I
highly reduced activity compared to wild-type
Euphorbia tirucalli
5.4.99.39
F728M
highly reduced activity compared to wild-type
Euphorbia tirucalli
5.4.99.39
F728W
highly reduced activity compared to wild-type
Euphorbia tirucalli
5.4.99.39
F728Y
activity comparable to wild-type
Euphorbia tirucalli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
5.4.99.39
(S)-2,3-oxidosqualene
-
729601
Euphorbia tirucalli
beta-amyrin
-
-
-
?