Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Agarwal, V.; Peck, S.C.; Chen, J.H.; Borisova, S.A.; Chekan, J.R.; van der Donk, W.A.; Nair, S.K.
    Structure and function of phosphonoacetaldehyde dehydrogenase: the missing link in phosphonoacetate formation (2014), Chem. Biol., 21, 125-135.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.3 ascorbate required Sinorhizobium meliloti

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.3 expressed in Escherichia coli Rosetta2 cells Sinorhizobium meliloti

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.2.1.3 apoenzyme and in complex with PnA und NAD+, hanging drop vapor diffusion method, using 22%-25% PEG3350, 0.1 M sodium cacodylate (pH 6.5), 0.2 M sodium phosphate dibasic, and 5%-10% glycerol Sinorhizobium meliloti

Protein Variants

EC Number Protein Variants Comment Organism
1.2.1.3 E385A the mutation results in 10fold reduction in kcat, with an approximately 6fold higher KM,NAD+ Sinorhizobium meliloti
1.2.1.3 N158A the mutation results in a 200fold decrease in kcat Sinorhizobium meliloti
1.2.1.3 R108A the mutation leads to a 40fold decrease in kcat and a 3fold increase in KM,PnAA Sinorhizobium meliloti
1.2.1.3 R290A the mutation results in a 20fold decrease in kcat with a slightly elevated KM,PnAA relative to wild type Sinorhizobium meliloti
1.2.1.3 R447A the mutation reduces the kcat 30fold and increases the KM,PnAA 50fold Sinorhizobium meliloti

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.3 0.0032
-
 phosphonoacetaldehyde wild type enzyme, with NAD+ in 50 mM HEPES, pH 7.5 supplemented with 10 mM MgSO4 for 5 min at 30°C Sinorhizobium meliloti
1.2.1.3 0.005
-
 phosphonoacetaldehyde mutant enzyme R290A, with NAD+ in 50 mM HEPES, pH 7.5 supplemented with 10 mM MgSO4 for 5 min at 30°C Sinorhizobium meliloti
1.2.1.3 0.009
-
 phosphonoacetaldehyde mutant enzyme R108A, with NAD+ in 50 mM HEPES, pH 7.5 supplemented with 10 mM MgSO4 for 5 min at 30°C Sinorhizobium meliloti
1.2.1.3 0.018
-
 NAD+ mutant enzyme R108A, with NAD+ in 50 mM HEPES, pH 7.5 supplemented with 10 mM MgSO4 for 5 min at 30°C Sinorhizobium meliloti
1.2.1.3 0.019
-
 phosphonoacetaldehyde mutant enzyme E385A, with NAD+ in 50 mM HEPES, pH 7.5 supplemented with 10 mM MgSO4 for 5 min at 30°C Sinorhizobium meliloti
1.2.1.3 0.029
-
 phosphonoacetaldehyde mutant enzyme N158A, with NAD+ in 50 mM HEPES, pH 7.5 supplemented with 10 mM MgSO4 for 5 min at 30°C Sinorhizobium meliloti
1.2.1.3 0.04
-
 NAD+ mutant enzyme N158A, with NAD+ in 50 mM HEPES, pH 7.5 supplemented with 10 mM MgSO4 for 5 min at 30°C Sinorhizobium meliloti
1.2.1.3 0.054
-
 NAD+ mutant enzyme R447A, with NAD+ in 50 mM HEPES, pH 7.5 supplemented with 10 mM MgSO4 for 5 min at 30°C Sinorhizobium meliloti
1.2.1.3 0.058
-
 NAD+ wild type enzyme, with NAD+ in 50 mM HEPES, pH 7.5 supplemented with 10 mM MgSO4 for 5 min at 30°C Sinorhizobium meliloti
1.2.1.3 0.075
-
 NAD+ mutant enzyme R290A, with NAD+ in 50 mM HEPES, pH 7.5 supplemented with 10 mM MgSO4 for 5 min at 30°C Sinorhizobium meliloti
1.2.1.3 0.15
-
 phosphonoacetaldehyde mutant enzyme R447A, with NAD+ in 50 mM HEPES, pH 7.5 supplemented with 10 mM MgSO4 for 5 min at 30°C Sinorhizobium meliloti
1.2.1.3 0.37
-
 NAD+ mutant enzyme E385A, with NAD+ in 50 mM HEPES, pH 7.5 supplemented with 10 mM MgSO4 for 5 min at 30°C Sinorhizobium meliloti

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.3 Fe2+ required Sinorhizobium meliloti

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.3 phosphonoacetaldehyde + NAD+ + H2O Sinorhizobium meliloti
-
 phosphonoacetate + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.3 Sinorhizobium meliloti Q92UV7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.3 Ni-NTA resin column chromatography and Superdex 75 gel filtration Sinorhizobium meliloti

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.3 3-oxopropylphosphonate + NAD+ + H2O
-
 Sinorhizobium meliloti ?
-
 ?
1.2.1.3 glyceraldehyde-3-phosphate + NAD+ + H2O
-
 Sinorhizobium meliloti ?
-
 ?
1.2.1.3 phosphonoacetaldehyde + NAD+ + H2O
-
 Sinorhizobium meliloti phosphonoacetate + NADH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.1.3 phnY
-
 Sinorhizobium meliloti
1.2.1.3 phosphonoacetaldehyde oxidase
-
 Sinorhizobium meliloti

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.1.3 0.01
-
 phosphonoacetaldehyde mutant enzyme N158A, with NAD+ in 50 mM HEPES, pH 7.5 supplemented with 10 mM MgSO4 for 5 min at 30°C Sinorhizobium meliloti
1.2.1.3 0.051
-
 phosphonoacetaldehyde mutant enzyme R108A, with NAD+ in 50 mM HEPES, pH 7.5 supplemented with 10 mM MgSO4 for 5 min at 30°C Sinorhizobium meliloti
1.2.1.3 0.076
-
 phosphonoacetaldehyde mutant enzyme R447A, with NAD+ in 50 mM HEPES, pH 7.5 supplemented with 10 mM MgSO4 for 5 min at 30°C Sinorhizobium meliloti
1.2.1.3 0.12
-
 phosphonoacetaldehyde mutant enzyme R290A, with NAD+ in 50 mM HEPES, pH 7.5 supplemented with 10 mM MgSO4 for 5 min at 30°C Sinorhizobium meliloti
1.2.1.3 0.19
-
 phosphonoacetaldehyde mutant enzyme E385A, with NAD+ in 50 mM HEPES, pH 7.5 supplemented with 10 mM MgSO4 for 5 min at 30°C Sinorhizobium meliloti
1.2.1.3 2.2
-
 phosphonoacetaldehyde wild type enzyme, with NAD+ in 50 mM HEPES, pH 7.5 supplemented with 10 mM MgSO4 for 5 min at 30°C Sinorhizobium meliloti

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.3 NAD+
-
 Sinorhizobium meliloti