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Literature summary extracted from

  • Elgharbi, F.; Hmida-Sayari, A.; Sahnoun, M.; Kammoun, R.; Jlaeil, L.; Hassairi, H.; Bejar, S.
    Purification and biochemical characterization of a novel thermostable lichenase from Aspergillus niger US368 (2013), Carbohydr. Polym., 98, 967-975.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.73 food industry the enzyme is considered as a candidate for application particularly in the animal feed industry Aspergillus niger

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.73 Ca2+ 11% inhibition at 5 mM Aspergillus niger
3.2.1.73 Co2+ 52% inhibition at 5 mM Aspergillus niger
3.2.1.73 Cu2+ complete inhibition at 5 mM Aspergillus niger
3.2.1.73 Fe2+ complete inhibition at 5 mM Aspergillus niger
3.2.1.73 Mn2+ complete inhibition at 5 mM Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.73 additional information
-
 additional information Michaelis-Menten kinetics, 0.62 mg/ml with barley beta-glucan at pH 5.0, 60°C,and 0.38 mg/ml with lichenan at pH 5.0, 60°C Aspergillus niger

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.73 extracellular
-
 Aspergillus niger
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.73 additional information poor effects by Mg2+ and Zn2+ at 5 mM Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.73 Aspergillus niger
-
-
-
3.2.1.73 Aspergillus niger US368
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.73 native extracellular enzyme 14.24fold from culture supernatant by ultrafiltration and gel filtration to homogeneity Aspergillus niger

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.73 additional information optimization of production of beta-glucanase with barley flour as the sole carbon source Aspergillus niger
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.73 barley beta-glucan + H2O
-
 Aspergillus niger cellotriose + cellotetraose + cellobiose + D-glucose oligosaccharides (mainly trisaccharide and tetrasaccharide) followed by disaccharide (cellobiose) and glucose are formed as the major products of hydrolysis after a prolonged incubation of 4 h ?
3.2.1.73 barley beta-glucan + H2O
-
 Aspergillus niger US368 cellotriose + cellotetraose + cellobiose + D-glucose oligosaccharides (mainly trisaccharide and tetrasaccharide) followed by disaccharide (cellobiose) and glucose are formed as the major products of hydrolysis after a prolonged incubation of 4 h ?
3.2.1.73 lichenan + H2O
-
 Aspergillus niger ?
-
 ?
3.2.1.73 lichenan + H2O
-
 Aspergillus niger US368 ?
-
 ?
3.2.1.73 additional information the enzyme is a conformation-retaining and is only active toward glucan containing beta-1,3-1,4-linkages. No activity with laminarin and carboxymethyl cellulose Aspergillus niger ?
-
 ?
3.2.1.73 additional information the enzyme is a conformation-retaining and is only active toward glucan containing beta-1,3-1,4-linkages. No activity with laminarin and carboxymethyl cellulose Aspergillus niger US368 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.73 ? x *32000, SDS-PAGE Aspergillus niger

Synonyms

EC Number Synonyms Comment Organism
3.2.1.73 beta-1,3;1,4-glucanase
-
 Aspergillus niger
3.2.1.73 Lichenase
-
 Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.73 60
-
-
 Aspergillus niger

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.73 40 80 activity range, profile overview Aspergillus niger

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.73 50
-
 purified enzyme, pH 5.0, half-life is 150 min Aspergillus niger
3.2.1.73 60
-
 purified enzyme, pH 5.0, half-life is 135 min Aspergillus niger
3.2.1.73 65
-
 purified enzyme, pH 5.0, half-life is 90 min Aspergillus niger
3.2.1.73 70
-
 purified enzyme, pH 5.0, half-life is 30 min Aspergillus niger

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.73 13.32
-
 lichenan pH 5.0, 60°C Aspergillus niger
3.2.1.73 19.11
-
 Barley beta-glucan pH 5.0, 60°C Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.73 5
-
-
 Aspergillus niger

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.73 3 6.5 activity range, profile overview Aspergillus niger

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.73 3 10 purified enzyme, 37°C, 1 h, completely stable Aspergillus niger