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Literature summary extracted from
- Molecular identification of a polyM-specific alginate lyase from Pseudomonas sp. strain KS-408 for degradation of glycosidic linkages between two mannuronates or mannuronate and guluronate in alginate (2011), Can. J. Microbiol., 57, 1032-1041.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.2.2.11 | - |
Pseudomonas sp. |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 4.2.2.11 | homology modeling of structure. Residues Asn198, His199, Arg246, and Tyr253 are conserved for the catalytic active site | Pseudomonas sp. |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.11 | 42400 | - |
x * 45500, recombinant enzyme with signal peptide, x * 42400, recombinant enzyme without signal peptide, calulated | Pseudomonas sp. |
| 4.2.2.11 | 45500 | - |
x * 45500, recombinant enzyme with signal peptide, x * 42400, recombinant enzyme without signal peptide, calulated | Pseudomonas sp. |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.2.11 | Pseudomonas sp. | G9J5R3 | - |
- |
| 4.2.2.11 | Pseudomonas sp. KS-408 | G9J5R3 | - |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 4.2.2.11 | proteolytic modification | recombinant enzyme with signal peptide and without signal peptide with the His-tag consist of 393 and 372 amino acids, respectively | Pseudomonas sp. |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.2.11 | alginate | - |
Pseudomonas sp. | ? | enzyme cleaves the glycosidic linkages between two mannuronates (mannuronate-beta(1-4)-mannuronate) or mannuronate and guluronate (mannuronate-beta(1-4)-guluronate) | ? |  |
| 4.2.2.11 | alginate | - |
Pseudomonas sp. KS-408 | ? | enzyme cleaves the glycosidic linkages between two mannuronates (mannuronate-beta(1-4)-mannuronate) or mannuronate and guluronate (mannuronate-beta(1-4)-guluronate) | ? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.2.2.11 | ? | x * 45500, recombinant enzyme with signal peptide, x * 42400, recombinant enzyme without signal peptide, calulated | Pseudomonas sp. |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.2.2.11 | Aly1 | - |
Pseudomonas sp. |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.11 | 40 | - |
stable for at least 30 min | Pseudomonas sp. |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 4.2.2.11 | Pseudomonas sp. | recombinant enzyme without signal peptide, calculated | - |
8.5 |
| 4.2.2.11 | Pseudomonas sp. | recombinant enzyme with signal peptide, calculated | - |
8.6 |
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