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Literature summary extracted from

  • Zhao, Q.; Su, Y.; Wang, Z.; Chen, C.; Wu, T.; Huang, Y.
    Identification of glutathione (GSH)-independent glyoxalase III from Schizosaccharomyces pombe (2014), BMC Evol. Biol., 14, 86.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.1.130 bovine serum albumin
-
 Schizosaccharomyces pombe
4.2.1.130 bovine serum albumin
-
 Saccharomyces cerevisiae
4.2.1.130 additional information the enzyme shows GSH-independent glyoxalase activity Schizosaccharomyces pombe
4.2.1.130 additional information the enzyme shows GSH-independent glyoxalase activity Saccharomyces cerevisiae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.130 expression of C-terminally GFP-tagged SpDJ-1, expression of wild-type and mutant SpDj-1 proteins in Escherichia coli strain BL21(DE3), detailed phylogenetic analysis of GLOIII, DJ-1 and HSP31 proteins, overview Schizosaccharomyces pombe
4.2.1.130 gene hsp3101 encoding a Hsp31 protein, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, recombinant overexpression of Schizosaccharomyces pombe hsp3101 in Schizosaccharomyces pombe wild-type cells or GLO1 deletion cells Schizosaccharomyces pombe
4.2.1.130 gene hsp3101, expression of C-terminally GFP-tagged HSP31 proteins, expression of HSP3101 protein in Escherichia coli strain BL21(DE3), detailed phylogenetic analysis of GLOIII, DJ-1 and HSP31 proteins, overview Schizosaccharomyces pombe
4.2.1.130 gene hsp3102 encoding a Hsp31 protein, sequence comparisons, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant overexpression of Schizosaccharomyces pombe hsp3101 in Schizosaccharomyces pombe wild-type cells or GLO1 deletion cells Schizosaccharomyces pombe
4.2.1.130 gene hsp3106 encoding DJ-1 protein, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis Schizosaccharomyces pombe
4.2.1.130 gene ScHSP31 encoding Hsp31 protein, sequence comparisons, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant overexpression of Schizosaccharomyces pombe hsp3101 in Schizosaccharomyces pombe wild-type cells or GLO1 deletion cells Saccharomyces cerevisiae
4.2.1.130 gene ScHSP31, expression in Escherichia coli strain BL21(DE3), detailed phylogenetic analysis of GLOIII, DJ-1 and HSP31 proteins, overview. Overexpression of ScHSP31 can confer methylglyoxal and glyoxal resistance on either wild-type Schizosaccharomyces pombe cells or GLO1-deletion of Schizosaccharomyces pombe Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.130 C111A site-directed mutagenesis, catalytically inactive mutant Schizosaccharomyces pombe
4.2.1.130 E16A site-directed mutagenesis, catalytically inactive mutant Schizosaccharomyces pombe
4.2.1.130 H130A site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Schizosaccharomyces pombe
4.2.1.130 additional information overexpression of Saccharomyces cerevisiae gene ScHSP31 can confer methylglyoxal and glyoxal resistance on either wild-type Schizosaccharomyces pombe cells or GLO1-deletion of Schizosaccharomyces pombe Schizosaccharomyces pombe
4.2.1.130 additional information overexpression of Saccharomyces cerevisiae ScHSP31 confers methylglyoxal and glyoxal resistance on either wild-type Schizosaccharomyces pombe cells or GLO1 deletion cells of Schizosaccharomyces pombe Saccharomyces cerevisiae
4.2.1.130 additional information overexpression of Schizosaccharomyces pombe hsp3101 confers methylglyoxal and glyoxal resistance on either wild-type Schizosaccharomyces pombe cells or GLO1 deletion cells of Schizosaccharomyces pombe Schizosaccharomyces pombe
4.2.1.130 additional information overexpression of Schizosaccharomyces pombe hsp3102 confers methylglyoxal and glyoxal resistance on either wild-type Schizosaccharomyces pombe cells or GLO1 deletion cells of Schizosaccharomyces pombe Schizosaccharomyces pombe

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.130 1.5
-
 methylglyoxal recombinant ScHsp31, pH and temperature not specified in the publication Saccharomyces cerevisiae
4.2.1.130 10.8
-
 methylglyoxal recombinant wild-type SpDJ-1, pH and temperature not specified in the publication Schizosaccharomyces pombe
4.2.1.130 31.1
-
 methylglyoxal recombinant Hsp3101, pH and temperature not specified in the publication Schizosaccharomyces pombe
4.2.1.130 37.3
-
 methylglyoxal recombinant mutant SpDJ-1 H130A, pH and temperature not specified in the publication Schizosaccharomyces pombe
4.2.1.130 58
-
 methylglyoxal recombinant Hsp3102, pH and temperature not specified in the publication Schizosaccharomyces pombe

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.1.130 cytoplasm
-
 Schizosaccharomyces pombe 5737
-
4.2.1.130 additional information recombinant GFP-tagged Hsp3102 is only localized in the cytoplasm, not in the nucleus Schizosaccharomyces pombe
-
-
4.2.1.130 nucleus
-
 Schizosaccharomyces pombe 5634
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.130 (R)-lactate Schizosaccharomyces pombe glyoxalase III (GLO3) converts methylglyoxal directly into D-lactate in a single step, independent of glutathione GSH 2-oxopropanal + H2O
-
 ?
4.2.1.130 (R)-lactate Saccharomyces cerevisiae glyoxalase III (GLO3) converts methylglyoxal directly into D-lactate in a single step, independent of glutathione GSH 2-oxopropanal + H2O
-
 ?
4.2.1.130 (R)-lactate Schizosaccharomyces pombe 972 glyoxalase III (GLO3) converts methylglyoxal directly into D-lactate in a single step, independent of glutathione GSH 2-oxopropanal + H2O
-
 ?
4.2.1.130 acetate Schizosaccharomyces pombe
-
 2-oxoethanal + H2O
-
 ?
4.2.1.130 acetate Saccharomyces cerevisiae
-
 2-oxoethanal + H2O
-
 ?
4.2.1.130 acetate Schizosaccharomyces pombe 972
-
 2-oxoethanal + H2O
-
 ?
4.2.1.130 methylglyoxal + H2O Schizosaccharomyces pombe
-
 (R)-lactate
-
 ?
4.2.1.130 methylglyoxal + H2O Saccharomyces cerevisiae
-
 (R)-lactate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.130 Saccharomyces cerevisiae Q04432
-
-
4.2.1.130 Saccharomyces cerevisiae Q04432 gene ScHSP31
-
4.2.1.130 Schizosaccharomyces pombe
-
 genes hsp3101 and hsp3102
-
4.2.1.130 Schizosaccharomyces pombe O74914
-
-
4.2.1.130 Schizosaccharomyces pombe Q09675
-
-
4.2.1.130 Schizosaccharomyces pombe Q10356
-
-
4.2.1.130 Schizosaccharomyces pombe 972 O74914
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.130 (R)-lactate
-
 Schizosaccharomyces pombe 2-oxopropanal + H2O
-
 ?
4.2.1.130 (R)-lactate
-
 Saccharomyces cerevisiae 2-oxopropanal + H2O
-
 ?
4.2.1.130 (R)-lactate glyoxalase III (GLO3) converts methylglyoxal directly into D-lactate in a single step, independent of glutathione GSH Schizosaccharomyces pombe 2-oxopropanal + H2O
-
 ?
4.2.1.130 (R)-lactate glyoxalase III (GLO3) converts methylglyoxal directly into D-lactate in a single step, independent of glutathione GSH Saccharomyces cerevisiae 2-oxopropanal + H2O
-
 ?
4.2.1.130 (R)-lactate
-
 Schizosaccharomyces pombe 972 2-oxopropanal + H2O
-
 ?
4.2.1.130 (R)-lactate glyoxalase III (GLO3) converts methylglyoxal directly into D-lactate in a single step, independent of glutathione GSH Schizosaccharomyces pombe 972 2-oxopropanal + H2O
-
 ?
4.2.1.130 acetate
-
 Schizosaccharomyces pombe 2-oxoethanal + H2O
-
 ?
4.2.1.130 acetate
-
 Saccharomyces cerevisiae 2-oxoethanal + H2O
-
 ?
4.2.1.130 acetate
-
 Schizosaccharomyces pombe 972 2-oxoethanal + H2O
-
 ?
4.2.1.130 methylglyoxal + H2O
-
 Schizosaccharomyces pombe (R)-lactate
-
 ?
4.2.1.130 methylglyoxal + H2O
-
 Saccharomyces cerevisiae (R)-lactate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.130 DJ-1
-
 Schizosaccharomyces pombe
4.2.1.130 GLO3
-
 Schizosaccharomyces pombe
4.2.1.130 GLO3
-
 Saccharomyces cerevisiae
4.2.1.130 glutathione-independent glyoxalase III
-
 Schizosaccharomyces pombe
4.2.1.130 glutathione-independent glyoxalase III
-
 Saccharomyces cerevisiae
4.2.1.130 glyoxalase III
-
 Schizosaccharomyces pombe
4.2.1.130 glyoxalase III
-
 Saccharomyces cerevisiae
4.2.1.130 GSH-independent glyoxalase III
-
 Schizosaccharomyces pombe
4.2.1.130 GSH-independent glyoxalase III
-
 Saccharomyces cerevisiae
4.2.1.130 Hsp31
-
 Saccharomyces cerevisiae
4.2.1.130 Hsp3101
-
 Schizosaccharomyces pombe
4.2.1.130 Hsp3102
-
 Schizosaccharomyces pombe
4.2.1.130 hsp3106
-
 Schizosaccharomyces pombe
4.2.1.130 SpDJ-1
-
 Schizosaccharomyces pombe

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.130 0.518
-
 methylglyoxal recombinant Hsp3101, pH and temperature not specified in the publication Schizosaccharomyces pombe
4.2.1.130 0.967
-
 methylglyoxal recombinant Hsp3102, pH and temperature not specified in the publication Schizosaccharomyces pombe
4.2.1.130 1.09
-
 methylglyoxal recombinant mutant SpDJ-1 H130A, pH and temperature not specified in the publication Schizosaccharomyces pombe
4.2.1.130 1.25
-
 methylglyoxal recombinant ScHsp31, pH and temperature not specified in the publication Saccharomyces cerevisiae
4.2.1.130 1.43
-
 methylglyoxal recombinant wild-type SpDJ-1, pH and temperature not specified in the publication Schizosaccharomyces pombe

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.1.130 additional information the enzyme shows GSH-independent glyoxalase activity Schizosaccharomyces pombe
4.2.1.130 additional information the enzyme shows GSH-independent glyoxalase activity Saccharomyces cerevisiae

General Information

EC Number General Information Comment Organism
4.2.1.130 evolution detailed phylogenetic analysis of GLOIII, DJ-1 and HSP31 proteins in fungi, overview Schizosaccharomyces pombe
4.2.1.130 evolution detailed phylogenetic analysis of GLOIII, DJ-1 and HSP31 proteins in fungi, overview Saccharomyces cerevisiae
4.2.1.130 evolution fungal Hsp31 proteins are the major glyoxalases III GLO3 that may have some role in protecting cells from reactive carbonyl species toxicity in fungi. The GLO3 activity of Hsp31 proteins may have evolved independently from GLO3 activity of DJ-1 proteins. DJ-1 and Hsp31 proteins belong to different subfamilies of the DJ-1/Hsp31/PfpI superfamily, which encompasses a wide variety of functionally diverse proteins, detailed phylogenetic analysis of DJ-1 and Hsp31 proteins, distribution of Hsp31 proteins in fungi, overview Schizosaccharomyces pombe
4.2.1.130 evolution fungal Hsp31 proteins are the major glyoxalases III GLO3 that may have some role in protecting cells from reactive carbonyl species toxicity in fungi. The GLO3 activity of Hsp31 proteins may have evolved independently from GLO3 activity of DJ-1 proteins. DJ-1 and Hsp31 proteins belong to different subfamilies of the DJ-1/Hsp31/PfpI superfamily, which encompasses a wide variety of functionally diverse proteins, detailed phylogenetic analysis of DJ-1 and Hsp31 proteins, distribution of Hsp31 proteins in fungi, overview Saccharomyces cerevisiae
4.2.1.130 additional information two Schizosaccharomyces pombe Hsp31 proteins (Hsp3101 and Hsp3102) and one Saccharomyces cerevisiae Hsp31 protein (ScHsp31) display significantly higher in vitro GLO3 activity than Schizosaccharomyces pombe DJ-1 (SpDJ-1) Schizosaccharomyces pombe
4.2.1.130 additional information two Schizosaccharomyces pombe Hsp31 proteins (Hsp3101 and Hsp3102) and one Saccharomyces cerevisiae Hsp31 protein (ScHsp31) display significantly higher in vitro GLO3 activity than Schizosaccharomyces pombe DJ-1 (SpDJ-1) Saccharomyces cerevisiae
4.2.1.130 additional information both DJ-1 and Hsp31 proteins possess the Glu-Cys-His catalytic triad, Glu16-Cys111-His130 in SpDJ-1 Schizosaccharomyces pombe
4.2.1.130 additional information both DJ-1 and Hsp31 proteins possess the Glu-Cys-His catalytic triad, Glu16-Cys111-His130 in SpDJ-1 Saccharomyces cerevisiae
4.2.1.130 physiological function glyoxalase III is responsible for the detoxification of reactive carbonyl species, e.g. methylglyoxal and glyoxal, via the GSH-independent pathway, overview Schizosaccharomyces pombe
4.2.1.130 physiological function glyoxalase III is responsible for the detoxification of reactive carbonyl species, e.g. methylglyoxal and glyoxal, via the GSH-independent pathway, overview Saccharomyces cerevisiae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.1.130 0.03
-
 methylglyoxal recombinant mutant SpDJ-1 H130A, pH and temperature not specified in the publication Schizosaccharomyces pombe
4.2.1.130 0.132
-
 methylglyoxal recombinant wild-type SpDJ-1, pH and temperature not specified in the publication Schizosaccharomyces pombe
4.2.1.130 0.35
-
 methylglyoxal recombinant Hsp3102, pH and temperature not specified in the publication Schizosaccharomyces pombe
4.2.1.130 0.383
-
 methylglyoxal recombinant Hsp3101, pH and temperature not specified in the publication Schizosaccharomyces pombe
4.2.1.130 0.85
-
 methylglyoxal recombinant ScHsp31, pH and temperature not specified in the publication Saccharomyces cerevisiae