BRENDA - Enzyme Database

Enzyme activity evaluation of organic solvent-treated phenylalanine ammonia lyase

Quinn, A.J.; Pickup, M.J.; DCunha, G.B.; Biotechnol. Prog. 27, 1554-1560 (2012)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.3.1.24
acetone
-
Rhodotorula glutinis
4.3.1.24
Benzene
-
Rhodotorula glutinis
4.3.1.24
butanol
-
Rhodotorula glutinis
Organism
EC Number
Organism
UniProt
Commentary
Textmining
4.3.1.24
Rhodotorula glutinis
-
-
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.3.1.24
4.23
-
forward activity, substrate: L-phenylalanine methyl ester, pH 8.8, 30°C
Rhodotorula glutinis
4.3.1.24
4.67
-
forward activity, substrate: L-Phe, pH 8.8, 30°C
Rhodotorula glutinis
4.3.1.24
119
-
forward activity, substrate: L-phenylalanine methyl ester, pH 9, 30°C
Rhodotorula glutinis
4.3.1.24
145
-
forward activity, substrate: L-Phe, pH 9, 30°C
Rhodotorula glutinis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
4.3.1.24
L-Phe
-
729481
Rhodotorula glutinis
(E)-cinnamate + NH3
-
-
-
r
4.3.1.24
L-phenylalanine methyl ester
-
729481
Rhodotorula glutinis
?
-
-
-
r
4.3.1.24
additional information
among the water miscible solvents, ethanol treated and methanol treated enzymes supported maximum PAL forward and reverse activities, respectively. In the water immiscible solvents category, heptane-treated enzyme exhibits maximal activity for both PAL forward and reverse reactions
729481
Rhodotorula glutinis
?
-
-
-
-
Synonyms
EC Number
Synonyms
Commentary
Organism
4.3.1.24
PAL
-
Rhodotorula glutinis
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.3.1.24
30
-
assay at
Rhodotorula glutinis
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.3.1.24
8.8
-
forward reaction
Rhodotorula glutinis
4.3.1.24
9
-
reverse reaction
Rhodotorula glutinis
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.3.1.24
acetone
-
Rhodotorula glutinis
4.3.1.24
Benzene
-
Rhodotorula glutinis
4.3.1.24
butanol
-
Rhodotorula glutinis
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.3.1.24
4.23
-
forward activity, substrate: L-phenylalanine methyl ester, pH 8.8, 30°C
Rhodotorula glutinis
4.3.1.24
4.67
-
forward activity, substrate: L-Phe, pH 8.8, 30°C
Rhodotorula glutinis
4.3.1.24
119
-
forward activity, substrate: L-phenylalanine methyl ester, pH 9, 30°C
Rhodotorula glutinis
4.3.1.24
145
-
forward activity, substrate: L-Phe, pH 9, 30°C
Rhodotorula glutinis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
4.3.1.24
L-Phe
-
729481
Rhodotorula glutinis
(E)-cinnamate + NH3
-
-
-
r
4.3.1.24
L-phenylalanine methyl ester
-
729481
Rhodotorula glutinis
?
-
-
-
r
4.3.1.24
additional information
among the water miscible solvents, ethanol treated and methanol treated enzymes supported maximum PAL forward and reverse activities, respectively. In the water immiscible solvents category, heptane-treated enzyme exhibits maximal activity for both PAL forward and reverse reactions
729481
Rhodotorula glutinis
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.3.1.24
30
-
assay at
Rhodotorula glutinis
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.3.1.24
8.8
-
forward reaction
Rhodotorula glutinis
4.3.1.24
9
-
reverse reaction
Rhodotorula glutinis