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Literature summary extracted from

  • Yao, M.Z.; Wang, X.; Wang, W.; Fu, Y.J.; Liang, A.H.
    Improving the thermostability of Escherichia coli phytase, appA, by enhancement of glycosylation (2013), Biotechnol. Lett., 35, 1669-1676.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.8 expression in Pichia pastoris Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.8 Q258N substitution introduced to enhance the glycosylation activity upon expression in Pichia pastoris Escherichia coli
3.1.3.8 Q258N/Q349N substitutions introduced to enhance the glycosylation activity upon expression in Pichia pastoris. Mutant displays 40% enhancement in thermostability compared to wild-type Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.8 0.43
-
 myo-inositol hexakisphosphate mutant Q258N/Q349N, pH 4.5, 37°C Escherichia coli
3.1.3.8 0.48
-
 myo-inositol hexakisphosphate wild-type, pH 4.5, 37°C Escherichia coli
3.1.3.8 0.52
-
 myo-inositol hexakisphosphate mutant Q258N, pH 4.5, 37°C Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.8 53000
-
 x * 53000, SDS-PAGE, wild-type Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.8 Escherichia coli
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.3.8 glycoprotein after treatment with EndoH, protein gives one band of 50 kDa in SDS-PAGE Escherichia coli

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.8 recombinant protein Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.8 3137
-
 mutant Q258N/Q349N, pH 4.5, 37°C Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
 Escherichia coli myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.8 ? x * 53000, SDS-PAGE, wild-type Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
3.1.3.8 AppA
-
 Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.8 67.5
-
 melting temperature, wild-type Escherichia coli
3.1.3.8 72.2
-
 melting temperature, mutant Q258N/Q349N Escherichia coli
3.1.3.8 85
-
 10 min, 60% residual activity for mutant Q258N/Q349N, 5 min, 30% residual activiy for wild-type Escherichia coli
3.1.3.8 100
-
 10 min, 40% residual activity for mutant Q258N/Q349N Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.8 2660
-
 myo-inositol hexakisphosphate mutant Q258N, pH 4.5, 37°C Escherichia coli
3.1.3.8 3058
-
 myo-inositol hexakisphosphate mutant Q258N/Q349N, pH 4.5, 37°C Escherichia coli
3.1.3.8 3397
-
 myo-inositol hexakisphosphate wild-type, pH 4.5, 37°C Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.8 4.5
-
 both wild-typ and mutants Q258N and Q258N/Q349N Escherichia coli