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Literature summary extracted from

  • Huang, L.; Shizume, A.; Nogawa, M.; Taguchi, G.; Shimosaka, M.
    Heterologous expression and functional characterization of a novel chitinase from the chitinolytic bacterium Chitiniphilus shinanonensis (2012), Biosci. Biotechnol. Biochem., 76, 517-522.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.14 gene chiC, DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha Chitiniphilus shinanonensis
3.2.1.14 gene chiD, DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha Chitiniphilus shinanonensis
3.2.1.14 gene chiE, DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha Chitiniphilus shinanonensis
3.2.1.14 gene chiF, DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha Chitiniphilus shinanonensis
3.2.1.14 gene chiG, DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha Chitiniphilus shinanonensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.14 54400
-
 x * 54400, recombinant enzyme, SDS-PAGE Chitiniphilus shinanonensis
3.2.1.14 74400
-
 x * 74400, recombinant enzyme, SDS-PAGE Chitiniphilus shinanonensis
3.2.1.14 80800
-
 x * 80800, recombinant enzyme, SDS-PAGE Chitiniphilus shinanonensis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Chitiniphilus shinanonensis F8WSQ0 gene chiC encoded in the chiCDEFG operon
-
3.2.1.14 Chitiniphilus shinanonensis F8WSQ1 gene chiD encoded in the chiCDEFG operon
-
3.2.1.14 Chitiniphilus shinanonensis F8WSQ2 gene chiE encoded in the chiCDEFG operon
-
3.2.1.14 Chitiniphilus shinanonensis F8WSQ3 gene chiF encoded in the chiCDEFG operon
-
3.2.1.14 Chitiniphilus shinanonensis F8WSQ4 gene chiG encoded in the chiCDEFG operon
-
3.2.1.14 Chitiniphilus shinanonensis SAY3 F8WSQ0 gene chiC encoded in the chiCDEFG operon
-
3.2.1.14 Chitiniphilus shinanonensis SAY3 F8WSQ1 gene chiD encoded in the chiCDEFG operon
-
3.2.1.14 Chitiniphilus shinanonensis SAY3 F8WSQ2 gene chiE encoded in the chiCDEFG operon
-
3.2.1.14 Chitiniphilus shinanonensis SAY3 F8WSQ3 gene chiF encoded in the chiCDEFG operon
-
3.2.1.14 Chitiniphilus shinanonensis SAY3 F8WSQ4 gene chiG encoded in the chiCDEFG operon
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.14 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by affinity chromatography Chitiniphilus shinanonensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 4-nitrophenyl beta-N,N',N''-triacetylchitotrioside + H2O
-
 Chitiniphilus shinanonensis ?
-
 ?
3.2.1.14 4-nitrophenyl beta-N,N',N''-triacetylchitotrioside + H2O
-
 Chitiniphilus shinanonensis SAY3 ?
-
 ?
3.2.1.14 4-nitrophenyl beta-N,N'-diacetylchitobioside + H2O
-
 Chitiniphilus shinanonensis ?
-
 ?
3.2.1.14 4-nitrophenyl beta-N,N'-diacetylchitobioside + H2O
-
 Chitiniphilus shinanonensis SAY3 ?
-
 ?
3.2.1.14 4-nitrophenyl beta-N,N'-diacetylchitobioside + H2O
-
 Chitiniphilus shinanonensis 4-nitrophenol + N,N'-diacetylchitobiose
-
 ?
3.2.1.14 4-nitrophenyl beta-N,N'-diacetylchitobioside + H2O
-
 Chitiniphilus shinanonensis SAY3 4-nitrophenol + N,N'-diacetylchitobiose
-
 ?
3.2.1.14 colloidal chitin + H2O from crab shell Chitiniphilus shinanonensis chitobiose + D-glucosamine
-
 ?
3.2.1.14 colloidal chitin + H2O from crab shell Chitiniphilus shinanonensis N-acetyl-beta-D-glucosamine + ? isozyme ChiG shows unique activity and completely splits the dimer into monomers ?
3.2.1.14 additional information no activity with colloidal chitin Chitiniphilus shinanonensis ?
-
 ?
3.2.1.14 additional information endochitinase that cleaves oligosaccharides with three or more N-acetyl-beta-D-glucosamine monomers, no activity with 4-nitrophenyl N-acetylglucosamine Chitiniphilus shinanonensis ?
-
 ?
3.2.1.14 additional information endochitinase that cleaves oligosacchrides with three or more N-acetyl-beta-D-glucosamine monomers, no activity with 4-nitrophenyl N-acetylglucosamine Chitiniphilus shinanonensis ?
-
 ?
3.2.1.14 additional information endochitinase, isozyme ChiG shows unique activity and completely splits the dimer into monomers, no activity with 4-nitrophenyl N-acetylglucosamine Chitiniphilus shinanonensis ?
-
 ?
3.2.1.14 additional information the recombinant isozyme ChiF has no chitinolytic activity Chitiniphilus shinanonensis ?
-
 ?
3.2.1.14 additional information endochitinase that cleaves oligosaccharides with three or more N-acetyl-beta-D-glucosamine monomers, no activity with 4-nitrophenyl N-acetylglucosamine Chitiniphilus shinanonensis SAY3 ?
-
 ?
3.2.1.14 additional information no activity with colloidal chitin Chitiniphilus shinanonensis SAY3 ?
-
 ?
3.2.1.14 additional information endochitinase that cleaves oligosacchrides with three or more N-acetyl-beta-D-glucosamine monomers, no activity with 4-nitrophenyl N-acetylglucosamine Chitiniphilus shinanonensis SAY3 ?
-
 ?
3.2.1.14 additional information the recombinant isozyme ChiF has no chitinolytic activity Chitiniphilus shinanonensis SAY3 ?
-
 ?
3.2.1.14 additional information endochitinase, isozyme ChiG shows unique activity and completely splits the dimer into monomers, no activity with 4-nitrophenyl N-acetylglucosamine Chitiniphilus shinanonensis SAY3 ?
-
 ?
3.2.1.14 N,N',N'',N''', N''''-pentaacetylchitopentaose + H2O
-
 Chitiniphilus shinanonensis 5 N-acetyl-D-glucosamine
-
 ?
3.2.1.14 N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
-
 Chitiniphilus shinanonensis N,N'-diacetylchitobiose + GlcNAc
-
 ?
3.2.1.14 N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
-
 Chitiniphilus shinanonensis SAY3 N,N'-diacetylchitobiose + GlcNAc
-
 ?
3.2.1.14 N,N',N'',N'''-tetraacetylchitotetraose + 3 H2O
-
 Chitiniphilus shinanonensis 4 N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.14 N,N',N''-triacetylchitotriose + 2 H2O
-
 Chitiniphilus shinanonensis 3 N-acetyl-D-glucosamine
-
 ?
3.2.1.14 N,N',N''-triacetylchitotriose + 2 H2O
-
 Chitiniphilus shinanonensis SAY3 3 N-acetyl-D-glucosamine
-
 ?
3.2.1.14 N,N'-diacetylchitobiose + H2O
-
 Chitiniphilus shinanonensis 2 acetyl-D-glucosamine
-
 ?
3.2.1.14 partially N-acetylated chitosan + H2O
-
 Chitiniphilus shinanonensis ?
-
 ?
3.2.1.14 partially N-acetylated chitosan + H2O
-
 Chitiniphilus shinanonensis SAY3 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.14 ? x * 54400, recombinant enzyme, SDS-PAGE Chitiniphilus shinanonensis
3.2.1.14 ? x * 74400, recombinant enzyme, SDS-PAGE Chitiniphilus shinanonensis
3.2.1.14 ? x * 80800, recombinant enzyme, SDS-PAGE Chitiniphilus shinanonensis
3.2.1.14 More isozyme domain structures, overview Chitiniphilus shinanonensis

Synonyms

EC Number Synonyms Comment Organism
3.2.1.14 ChiC
-
 Chitiniphilus shinanonensis
3.2.1.14 ChiD
-
 Chitiniphilus shinanonensis
3.2.1.14 ChiE
-
 Chitiniphilus shinanonensis
3.2.1.14 ChiF
-
 Chitiniphilus shinanonensis
3.2.1.14 ChiG
-
 Chitiniphilus shinanonensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.14 40
-
-
 Chitiniphilus shinanonensis
3.2.1.14 43
-
-
 Chitiniphilus shinanonensis
3.2.1.14 45
-
-
 Chitiniphilus shinanonensis
3.2.1.14 55
-
-
 Chitiniphilus shinanonensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 5
-
-
 Chitiniphilus shinanonensis

General Information

EC Number General Information Comment Organism
3.2.1.14 evolution the enzyme belongs to the glycosyl hydrolase family GH18 Chitiniphilus shinanonensis
3.2.1.14 evolution the enzymes of the operon chiCDEFG belong to the glycosyl hydrolase family GH18, while chiF encodes a polypeptide with two chitin-binding domains, but no catalytic domain Chitiniphilus shinanonensis
3.2.1.14 metabolism the isozymes encoded by chiCDEFG operon act in a cooperative manner in chitin degradation Chitiniphilus shinanonensis