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Literature summary extracted from

  • Hu, W.; Liu, N.; Tian, Y.; Zhang, L.
    Molecular cloning, expression, purification, and functional characterization of dammarenediol synthase from Panax ginseng (2013), BioMed Res. Int., 2013, 285740.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.125 DNA and amino acid sequence determination and analysis, recombinant expression of functional His-tagged enzyme in Escherichia coli strains Origami B (DE3) amd Rosetta (DE3) Panax ginseng

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.125 84600
-
 x * 84600, about, sequence calculation, x * 89000, recombinant His-tagged enzyme, SDS-PAGE Panax ginseng
4.2.1.125 89000
-
 x * 84600, about, sequence calculation, x * 89000, recombinant His-tagged enzyme, SDS-PAGE Panax ginseng

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.125 (3S)-2,3-epoxy-2,3-dihydrosqualene + H2O Panax ginseng
-
 dammarenediol II
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.125 Panax ginseng Q08IT1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.125 recombinant His-tagged enzyme from Escherichia coli strains Origami B (DE3) amd Rosetta (DE3) by nickel affinity chromatography Panax ginseng

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.125 root
-
 Panax ginseng
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.125 (3S)-2,3-epoxy-2,3-dihydrosqualene + H2O
-
 Panax ginseng dammarenediol II
-
 ?
4.2.1.125 additional information LC-MS analysis of substrate and product amounts Panax ginseng ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.125 ? x * 84600, about, sequence calculation, x * 89000, recombinant His-tagged enzyme, SDS-PAGE Panax ginseng

Synonyms

EC Number Synonyms Comment Organism
4.2.1.125 dammarenediol synthase
-
 Panax ginseng

General Information

EC Number General Information Comment Organism
4.2.1.125 metabolism the enzyme is involved in the ginsenoside biosynthetic pathway catalyzing the first committed step, the cyclization of 2,3-oxidosqualene to dammarenediol Panax ginseng
4.2.1.125 additional information close correlation of dammarenediol synthetase and saponin production in vitro Panax ginseng